目的 探究正畸牙移动中脂联素（adiponectin，APN）调控磷脂酰肌醇3激酶（Phosphatidy linositol 3 kinase，PI3K）/蛋白质丝氨酸-苏氨酸激酶（Protein serine threonine kinase，AKt）信号通路对牙周组织改建的影响。 方法 选取60只大鼠构建正畸牙移动模型并随机分为空白组、PBS组（注射PBS）、APN组（注射APN），以及APN+胰岛素生长因子1（Insulin growth factor 1，IGF-1）组（注射APN，同时给予PI3K/AKt通路激活剂IGF-1），每组15只。观察加力后1，7，14 d第1磨牙移动距离及破骨细胞数量，观察14 d时牙周组织形态，比较各组牙周组织PI3K、AKt mRNA表达及PI3K、p-PI3K、AKt、p-AKt蛋白表达。 结果 加力后7 d，14 d，APN组第1磨牙移动距离明显小于空白组（P<0.05），APN组新骨形成量及新骨矿化程度高于空白组、PBS组，APN组破骨细胞数量明显少于空白组（P<0.05）。APN组牙周组织PI3K、AKt mRNA及p-PI3K、p-AKt蛋白表达均明显低于空白组（P<0.05）。APN＋IGF-1组PI3K、AKt mRNA及p-PI3K、p-AKt蛋白表达均明显高于APN组（P<0.05）。 结论 APN可降低正畸牙移动大鼠模型压力侧破骨细胞数量及牙移动速度，在延缓正畸牙移动牙周组织改建过程中具有重要作用，其机制可能是通过抑制PI3K/AKt信号通路实现。
Abstract
Objective To investigate the effect of adiponectin (APN) regulating phosphatidylinositol 3 kinase (PI3K) / protein serine threonine kinase (AKt) signal pathway on periodontal tissue remodeling during orthodontic tooth movement. Methods Sixty rats were randomly divided into a blank group (untreated), a PBS group (injected with PBS), an APN group (injected with APN) and an APN + insulin growth factor-1 (IGF-1) (PI3K / Akt pathway activator) group, 15 mice in each group. The movement distance of the first molar and the number of osteoclasts in the three groups were observed at 1d, 7 d and 14 d, and the morphological changes of periodontal tissue in the three groups were observed at 14 d. The expressions of PI3K and AKt mRNA and PI3K, p-Pi3K, AKt and p-AKt protein in periodontal tissue were compared. Results At 7d and 14d, the movement distance of the first molar in APN group was significantly lower than that in blank group (P<0.05). The amount of new bone formation and the degree of new bone mineralization in APN group were higher than those in blank group and PBS group. At 7d and 14d, the number of osteoclasts in APN group was significantly lower than that in blank group (P<0.05). The expressions of PI3K, AKt mRNA and p-Pi3K, p-AKt protein in periodontal tissue in APN group were significantly lower than those in blank control group (P<0.05). The expressions of PI3K, AKt mRNA and p-Pi3K, p-AKt protein in APN + IGF-1 group were significantly higher than those in APN group (P<0.05). Conclusions APN can reduce the number of osteoclasts on the pressure side and the speed of tooth movement in the rat model of orthodontic tooth movement, and plays an important role in delaying the remodeling of periodontal tissue during orthodontic tooth movement. Its mechanism may be realized by inhibiting PI3K / AKt signal pathway
关键词
正畸牙移动； /
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脂联素； /
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磷脂酰肌醇3激酶； /
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蛋白质丝氨酸-苏氨酸激酶； /
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牙周组织改建
Key words
Orthodontic tooth movement; /
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Adiponectin; /
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Phosphatidylinositol 3 kinase; /
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Protein serine threonine kinase; /
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Periodontal tissue remodeling
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基金
江西省科技计划项目（20151BBG70242）；江西省卫健委科技计划项目（JX202130526）
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