抗炎治疗影响DEN诱导原发性肝癌进展的研究

doi:10.13418/j.issn.1001-165x.2017.01.008

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (1): 37-42.doi: 10.13418/j.issn.1001-165x.2017.01.008

抗炎治疗影响DEN诱导原发性肝癌进展的研究

李香芝¹，　钮红岺¹，　刘晓珑²，　刘立新²

1. 南方医科大学，广州 510515； 2. 南方医科大学第三附属医院，广州 510630

收稿日期:2016-11-10 出版日期:2017-01-25 发布日期:2017-02-22
通讯作者: 刘立新，教授，硕士生导师，Tel：020-62784433，E-mail：liulixin@tom.com
作者简介:共同第一作者：李香芝（1990-），女，湖南长沙市人，在读硕士研究生，主要从事肝脏慢性炎症对肝癌进展的研究，E-mail：y1664296431@163.com；钮红岺（1989-），男，河南安阳人，在读硕士研究生，主要从事乳腺癌转移，肝脏慢性炎症对肝癌进展的研究, E-mail：1264433707@qq.com
基金资助:
广东省医学科研基金（B2013261）

The effect of anti-inflammatory treatment on the progress of diethylnitrosamine induced primary liver cancer

LI Xiang-zhi¹，NIU Hong-ling¹，LIU Xiao-long²，LIU Li-xin²

1. Southern Medical University，Guangzhou 510515,China；2. The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China

Received:2016-11-10 Online:2017-01-25 Published:2017-02-22

摘要/Abstract

摘要：

目的观察抗炎治疗对二乙基亚硝胺（diethylnitrosamine,DEN）诱导原发性肝癌进展的影响。方法选取42只大鼠建立DEN诱导肝癌模型，随机分为3组，观察诱癌不同时期各组肝组织结节个数及肝组织结构的变化，用免疫组化检测3组CD68、CD206和CD34的阳性细胞表达，用western blot检测3组肝组织中TNF-α、IL10、TGF-β、HGF及NFκB等蛋白的相对表达。结果与对照组及葡醛内酯组相比，氢化可的松组结节个数更少，肝癌出现时间更晚，肝组织中CD68、CD206和CD34的阳性细胞表达更少，在诱癌第8~12周TNF-α、IL10、TGFβ、HGF和NFκB等蛋白在肝组织中的相对表达更少。结论抑制肝脏微环境中单核-巨噬细胞介导的慢性非特异性炎症能在诱癌早期延缓DEN诱导原发性肝癌的进展。

关键词: 抗炎治疗, 原发性肝癌, 二乙基亚硝胺

Abstract:

Objective　To investigate the influence of anti-inflammatory treatment on the progress of DEN induced primary liver cancer. 　Methods　42 rats which were fed with Diethylnitrosamine (DEN) solution to induce the rat model of liver cancer, and randomly divided into 3 groups. The nodule number and the structural change of liver tissue in each group were observed. CD68, CD206 and CD34 positive expression in liver tissue were detected by immunohistochemical staining in each group. Relative expression of TNF-α, IL10, TGF-β, HGF and NFκB in the liver tissue was detected by western blot in each group. Results　 Compared with the control group and glucurolactone group，hydrocortisone group's nodule number was fewer，the appearance time of liver cancer was postponed, the positive expression of CD68, CD206 and CD34 was fewer, the protein relative expression of TNF-α, IL10, TGF -β, HGF and NF kappa B was fewer at the 8th,10th, and 12th week.　Conclusion　Inhibiting the chronic nonspecific inflammation which is mediated by mononuclear - macrophage in liver microenvironment can delay the progress of the primary liver cancer in rats at an early stage which is induced by DEN.

Key words: Anti-inflammatory treatment, Primary liver cancer, Diethylnitrosamine (DEN)

李香芝,钮红岺,刘晓珑,刘立新. 抗炎治疗影响DEN诱导原发性肝癌进展的研究[J]. 中国临床解剖学杂志, 2017, 35(1): 37-42.

LI Xiang-zhi，NIU Hong-ling，LIU Xiao-long，LIU Li-xin. The effect of anti-inflammatory treatment on the progress of diethylnitrosamine induced primary liver cancer[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(1): 37-42.

抗炎治疗影响DEN诱导原发性肝癌进展的研究

The effect of anti-inflammatory treatment on the progress of diethylnitrosamine induced primary liver cancer

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