TAp73 和Beclin1在人骨肉瘤中表达及其临床意义
许遵营,吕海,史本超,胡争波,蔡琛,杨潇,靳安民,严鑫平
中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (1) : 68-72.
TAp73 和Beclin1在人骨肉瘤中表达及其临床意义
Expression of TAp73 and Beclin1 in human osteosarcoma and their clinical significance
目的 检测抑癌基因TAp73与自噬相关因子Beclin1在人骨肉瘤中的表达，探讨TAp73与肿瘤自噬的关系。 方法 采用免疫组织化学SP法检测TAp73、Beclin1在48例骨肉瘤患者的瘤体组织中的表达，结合患者的临床资料进行相关性分析。 结果 TAp73在人骨肉瘤组织中的阳性率54.17%，Beclin1的阳性率为62.50%，TAp73阳性表达，其Beclin1表达程度较高（Z=-2.146，P=0.032）。在人骨肉瘤中TAp73的表达程度与Beclin1的表达程度呈正相关（r=0.470，P=0.001）。TAp73、Beclin1阳性表达的患者总体生存率优于阴性表达（80.8% vs 50.0%，P=0.000; 80.0% vs 44.4%，P=0.001）。TAp73、Beclin1不同表达程度与患者的生存时间呈正相关（r=0.696，P=0.00；r=0.372，P=0.009）。 结论 在人骨肉瘤中TAp73可能与骨肉瘤中Beclin1依赖的肿瘤自噬活性上调有关。检测TAp73和Beclin1在人骨肉瘤中的表达水平有助于患者临床预后判断。
Objective To investigate the expression of TAp73 and Beclin1 in human osteosarcoma and their relationship. Methods The immunohistochemical SP method was used to detect the expressions of the TAp73 and Beclin1 proteins in 48 osteosarcoma patients’ tumor tissues, and the correlations of TAp73 and Beclin1 were analyzed with their clinical data. Results The positive rate of TAp73 was 54.17%, and the positive rate of Beclin1 was 60.42%. TAp73 positive expression, and the expression of Beclin1 were higher (Z=-2.146, P=0.032). The expression level of TAp73 was positively correlated with the expression of Beclin1 (P=0.001). The overall survival rate of patients with TAp73 and Beclin1 positive expression was better than that of negative expression (80.8% vs 50.0%, P=0.000, 80.0% vs 44.4%, P=0.001). The expression level of TAp73 and Beclin1 was positively correlated with the survival time of patients(r=0.696，P=0.00；r=0.372，P=0.009). Conclusion In human osteosarcoma, TAp73 may be related to the upregulation of Beclin1 dependent tumor autophagy activity. And to detect the expression of TAp73 and Beclin1 is helpful for the assessment of patients’ prognosis.
骨肉瘤 / 自噬 / TAp73 / Beclin1 / 预后
Osteosarcoma / Autophagy / TAp73 / Beclin1 / Prognosis
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国家自然科学基金（81470121）
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