低频脉冲电磁场影响超顺磁性氧化铁纳米颗粒标记BMSCs的增殖和成软骨分化

doi:10.13418/j.issn.1001-165x.2015.06.010

中国临床解剖学杂志 ›› 2015, Vol. 33 ›› Issue (6): 662-666.doi: 10.13418/j.issn.1001-165x.2015.06.010

低频脉冲电磁场影响超顺磁性氧化铁纳米颗粒标记BMSCs的增殖和成软骨分化

方楚玲¹，　龚望球¹，　黄婉静¹，　周彬¹，　栗园¹，　田京²

1.南方医科大学第二临床医学院，广州 510282；　2.南方医科大学珠江医院骨科，广州 510282

收稿日期:2015-03-04 出版日期:2015-11-25 发布日期:2015-12-18
通讯作者: 田京，教授，硕士生导师，E-mail:tianjing6723@aliyun.com.cn
作者简介:共同第一作者：方楚玲（1991-），女，广东人，南方医科大学第二临床医学院2010级本科双语班在读，E-mail：1508643192@qq.com；龚望求（1991-），女，湖南人，南方医科大学第二临床医学院2010级本科双语班在读，E-mail:1443076388@qq.com
基金资助:
广东省科技计划资助项目（2011B031800147）; 大学生创新实验项目基金（1212112013）

LFPEMFs effects on the proliferation and chondrocyte-like differentiation of BMSCs labeled with SPIO

FANG Chu-ling¹，GONG Wang-qiu¹，HUANG Wan-jing¹，ZHOU Bin¹，LI Yuan¹, TIAN Jing²

1. No.2 Clinical Medical College, Southern Medical University, Guangzhou 510282, China; 2. Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

Received:2015-03-04 Online:2015-11-25 Published:2015-12-18

摘要/Abstract

摘要：

目的　探讨外加低频脉冲电磁场（low frequency pulsed electromagnetic fields，LFPEMFs）对超顺磁性氧化铁纳米颗粒（superparamagnetic iron oxide nanoparticle，SPIO）标记的骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）增殖和成软骨分化的影响。方法　体外培养BMSCs并进行SPIO标记。将SPIO标记的细胞分为4组并给予不同干预: LFPEMFs组,成软骨诱导组,LFPEMFs+成软骨诱导组,对照组。采用CCK8检测各组细胞的增殖情况，PCR及免疫荧光染色检测BMSCs成软骨分化水平。结果 CCK8检测表明，LFPEMFs刺激促进细胞增殖；分化培养21 d时，LFPEMFs+成软骨诱导组细胞的Aggrecan 和CollagenⅡ表达量明显高于对照组（P<0.05）。结论 LFPEMFs刺激可以促进SPIO标记的BMSCs的增殖和成软骨分化。

关键词: LFPEMFs, BMSCs, 增殖, Aggrecan, CollagenⅡ

Abstract:

Objective　To explore the effects of low frequency pulsed electromagnetic fields（LFPEMFs）on the proliferation and chondrocyte-like cells differentiation of BMSCs labeled with superpar-amagnetic iron oxide nanoparticle（SPIO）. Methods Labeled BMSCs were developed and divided into four groups, followed by different treatments：LFPEMFs group, chondrocyte-induced group, LFPEMFs and chondrocyte-induced group, and control group. The proliferation and chondrocyte-like differentiation of cells were measured accordingly, adopting CCK8, PCR or immunofluorecence staining, respectively.　Results　Compared with control group, the proliferation of BMSCs increased significantly in LFPEMFs stimulated cells. The expression level of Aggrecan and Collagen Ⅱ mRNA and protein in groups with LFPEMFs stimulation was significantly higher (P<0.05).　Conclusions　This experiment proves that LFPEMFs can promote the proliferation and chondrocyte-like cells differentiation of SPIO labeled BMSCs.

Key words: LFPEMFs, BMSCs, Proliferation, Aggrecan, CollagenⅡ

方楚玲,龚望球,黄婉静,周彬,栗园,田京. 低频脉冲电磁场影响超顺磁性氧化铁纳米颗粒标记BMSCs的增殖和成软骨分化[J]. 中国临床解剖学杂志, 2015, 33(6): 662-666.

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低频脉冲电磁场影响超顺磁性氧化铁纳米颗粒标记BMSCs的增殖和成软骨分化

LFPEMFs effects on the proliferation and chondrocyte-like differentiation of BMSCs labeled with SPIO

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