电针对SAMP8小鼠行为学及APP mRNA和BACE-1 mRNA影响
Effects of electroacupuncture on expression of APP mRNA and BACE-1m RNA in SAMP8 mice
目的 探讨电针对SAMP8 小鼠行为学及淀粉样前体蛋白( amyloid precursor protein, APP) 和APP切割酶1(B site APP cleaving enzyme1, BACE-1)的影响，为临床治疗阿尔茨海默病病提供新的途径。 方法 将快速老化模型小鼠(SAMP8) 60只随机分成模型组、电针组和西药组，每组20只，正常老化小鼠 (SAMR1) 20只作为正常组。电针组电针刺激“百会”、 “肾俞”、“内关”、“大椎”穴；西药物组给予石杉碱甲 0.02 mg/kg剂量灌胃；正常组和模型组不给予治疗。治疗 20 d 后，用 Morris 水迷宫检测小鼠学习记忆能力，免疫组化定性检测小鼠海马APP和 BACE-1蛋白的表达，进一步实时荧光定量 PCR法测定APP mRNA和 BACE-1 mRNA 的表达。 结果 与模型组比较，西药组与电针组逃避潜伏期缩短，有效区停留时间和及跨越平台次数均显著增加(P＜0.05)，电针组APP mRNA 和 BACE-1 mRNA 相对表达量显著减少(P<0.05) 。 结论 电针可能抑制 SAMP8 小鼠脑内APP mRNA and BACE-1 mRNA表达，减少Aβ 生成，改善其学习记忆障碍。
Objective To explore the effects of electroacupuncture on behavior and APP mRNA and BACE-1 mRNA in senescence accelerated mouse (SAMP8). This provides a further evidence for therapy of Alzheimer's disease. Methods Sixty senescence accelerated mice prone 8 (SAMP8) were randomized into a model group，an electroacupuncture group and a medicine group. Twenty mice of senescence accelerated mouse / resistance 1(SAMP1) were in a normal group．In the electroacpuncture group，electroacupuncture was applied to "Baihui"，"Shenyu"，"Neiguan" and "Dazhui"．In the medicine group，the gastric infusion with Huperzine A was applied 0.02 mg/kg．The normal group and model group were not treated. After treatment for 20 days，Morris water maze test was used to determine the learning and memory ability of mice. The expression of APP and BACE-1 was determined with immunohistochemistry. The real-time fluorescent quantitative PCR was applied to determine the expression of APP mRNA and BACE-1 mRNA．Results Compared with the model group，the escaping latent period was reduced significantly in the medicine group and the electroacupuncture group，frequencies of leaping and the target quadrant swimming time was increased significantly(P＜0.05); the expressions of APP mRNA and BACE-1 mRNA were reduced significantly in the electroacupuncture group(P＜0.05). Conclusion Electroacupuncture has improvements in learning and memory disturbance probably through inhibiting APP mRNA and BACE-1 mRNA expression and reduced cerebral Aβ level in SAMP8 mice．
Alzheimerps disease / Amyloid precursor protein / B site APP cleaving enzyme1
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