Chinese Journal of Clinical Anatomy >
Protective role of sulphur-containing amino acids on sinoatrial node dysfunction in type 1 diabetes rat model
Received date: 2017-09-08
Online published: 2017-12-30
Objective To explore the effects of cysteine or taurine on sinoatrial node dysfunction in diabetic rats and the underlying mechanism. Methods SD rats were randomly divided into four groups:control group(C) diabetes mellits group(DM), diabetes mellits+cysteine group(DM+Cys), diabetes mellits+taurine group(DM+Tau). The blood content of SOD and MDA were assessed. The sinoatrial node function was detected by SNRT, CSNRT, SACT and IHR. We also measured the expression of SOD2, HO-1, Nox2, Nox4, HCN2 and HCN4. Results Compared with C group, the levels of SOD decreased, whereas MDA increased in DM group. The IHR was reduced with the prolonging of SNRT, CSNRT and SACT. The DM group exhibited down-regulated expression of SOD2, HO-1, HCN2 and HCN4 but up-regulated expression of NOX2 and NOX4 (P<0.05). Compared with DM group, DM+Cys and DM+Tau group showed increased SOD, prolonged IHR, up-regulated expression of SOD2, HO-1, HCN2 and HCN4, down-regulated expression of NOX2 and NOX4 (P<0.05). Conclusion Cysteine or taurine supplementation can reduce the oxidative stress, increased the expression of HCN2 and HCN4, improve the sinoatrial node dysfunction in diabetic rats.
Key words： Cysteine; Taurine; Sinoatrial node; Diabetes mellits
ZHOU Li , WANG Qing-zhi , WANG Zhi-yong . Protective role of sulphur-containing amino acids on sinoatrial node dysfunction in type 1 diabetes rat model[J]. Chinese Journal of Clinical Anatomy, 2017 , 35(6) : 661 -664 . DOI: 10.13418/j.issn.1001-165x.2017.06.014
[1] Tse G, Lai ET, Tse V, et al. Molecular and Electrophysiological Mechanisms Underlying Cardiac Arrhythmogenesis in Diabetes Mellitus[J]. J Diabetes Res, 2016, 2016: 2848759.
[2] Scicchitano P, Carbonara S, Ricci G, et al. HCN channels and heart rate[J]. Molecules, 2012, 17(4): 4225-4235.
[3] 王庆志, 周立, 王志勇, 等. 1型糖尿病大鼠左心室HCN2和HCN4重构[J]. 中国临床解剖学杂志, 2011, 29(3): 321-324.
[4] 钟妮尔, 郑小璞, 马爱群, 等. 链脲佐菌素诱导糖尿病大鼠窦房结HCN通道表达的变化[J]. 山西医科大学学报, 2016, 47(5): 406-410.
[5] Stadtman ER, Levine RL. Free radical-mediated oxidation of free amino acids and amino acid residues in proteins[J]. Amino Acids, 2003, 25(3-4): 207-218.
[6] Tappia PS, Xu YJ, Rodriguez-Leyva D, et al. Cardioprotective effects of cysteine alone or in combination with taurine in diabetes[J]. Physiol Res, 2013, 62(2): 171-178.
[7] 程功, 孙超峰, 陈新义, 等. 大鼠窦房结功能及其HCN4通道蛋白表达的增龄性变化[J]. 中华心律失常学杂志, 2008, 12(1): 26-29.
[8] Huang X, Zhong N, Zhang H, et al. Reduced expression of HCN channels in the sinoatrial node of streptozotocin-induced diabetic rats[J]. Can J Physiol Pharmacol, 2017, 95(5): 586-594.
[9] 赵云跃, 钟钧琳, 谢冬梅, 等. 曲美他嗪降低1型糖尿病大鼠心肌组织炎症反应和氧化应激水平[J]. 中山大学学报(医学科学版), 2016, 37(3): 390-395.
[10] 司立宁, 刘慧琴, 王晓丽, 等. 血红素加氧酶-1在糖尿病心肌病中的保护作用[J]. 青海医学院学报, 2016, 37(3): 170-174.
[11] Maalouf RM, Eid AA, Gorin YC, et al. Nox4-derived reactive oxygen species mediate cardiomyocyte injury in early type 1 diabetes[J]. Am J Physiol Cell Physiol, 2012, 302(3): 597-604.
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