Protective role of sulphur-containing amino acids on sinoatrial node dysfunction in type 1 diabetes rat model
ZHOU Li, WANG Qing-zhi, WANG Zhi-yong
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (6) : 661-664.
Protective role of sulphur-containing amino acids on sinoatrial node dysfunction in type 1 diabetes rat model
Objective To explore the effects of cysteine or taurine on sinoatrial node dysfunction in diabetic rats and the underlying mechanism. Methods SD rats were randomly divided into four groups:control group(C) diabetes mellits group(DM), diabetes mellits+cysteine group(DM+Cys), diabetes mellits+taurine group(DM+Tau). The blood content of SOD and MDA were assessed. The sinoatrial node function was detected by SNRT, CSNRT, SACT and IHR. We also measured the expression of SOD2, HO-1, Nox2, Nox4, HCN2 and HCN4. Results Compared with C group, the levels of SOD decreased, whereas MDA increased in DM group. The IHR was reduced with the prolonging of SNRT, CSNRT and SACT. The DM group exhibited down-regulated expression of SOD2, HO-1, HCN2 and HCN4 but up-regulated expression of NOX2 and NOX4 (P<0.05). Compared with DM group, DM+Cys and DM+Tau group showed increased SOD, prolonged IHR, up-regulated expression of SOD2, HO-1, HCN2 and HCN4, down-regulated expression of NOX2 and NOX4 (P<0.05). Conclusion Cysteine or taurine supplementation can reduce the oxidative stress, increased the expression of HCN2 and HCN4, improve the sinoatrial node dysfunction in diabetic rats.
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