大鼠脊髓及脑干内膀胱支配中枢的跨突触示踪研究

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (4): 435-440.

大鼠脊髓及脑干内膀胱支配中枢的跨突触示踪研究

盛珺¹，　肖燎原²，　张月雷¹，　林浩东¹，　侯春林¹

上海长征医院 1.骨科，2.肾内科，上海 200003

收稿日期:2013-04-15 出版日期:2013-07-25 发布日期:2013-07-24
通讯作者: 侯春林, 教授，博士生导师，E-mail:houchunlin@yahoo.　com.cn E-mail:xx_sj@hotmail.com
作者简介:共同第一作者：盛珺（1986-），男，四川成都人，临床医学八年制在读，主要研究方向：周围神经损伤的修复与重建，Tel：13661921564 肖燎原(1986-)，男，湖南益阳人，临床医学八年制在读，主要研究方向：泌尿系统疾病

Transsynaptic tracing of CNS neural circuitry involved in the innervation of bladder function in the adult rat brainstem and spinal cord

SHENG Jun¹，XIAO Liao-yuan²，ZHANG Yue-lei¹，LIN Hao-dong¹，HOU Chun-lin¹

1.Department of Orthopedics, Shanghai Changzheng Hospital; 2.Department of Nephrology, Shanghai Changzheng Hospital, Shanghai 200003, China

Received:2013-04-15 Online:2013-07-25 Published:2013-07-24

摘要/Abstract

摘要：

目的　跨突触示踪正常大鼠脊髓和脑干内膀胱支配相关中枢，为进一步阐明膀胱功能重建术后中枢重塑奠定研究基础。方法　成年雌性SD大鼠15只，膀胱壁内分三个点共注射GFP-PRV 4.5 μl（1×108 PFU /ml）。注射后不同时间（72、84、96、108、120 h）分别取大脑、脊髓及背根神经节，荧光显微镜下观察标记结果。结果　病毒注射后72~120 h，阳性神经元主要分布于L6~S1、L1~L2脊神经背根神经节； L6~S1、L1~L2脊髓节段骶副交感核、中间外侧核及后连合核；脑干的巴氏核、中缝巨细胞核、巨细胞网状核、锥旁网状结构、去甲肾上腺素能细胞群A5及A7、蓝斑、中脑导水管周围灰质和红核腹侧区域。结论　本实验中标记的相关核团与膀胱存在解剖上的神经联系，可能直接或间接参与膀胱的支配。

关键词: 假狂犬病毒, 膀胱, 排尿反射通路, 中枢神经系统

Abstract:

Objective　To identify the CNS neural circuitry involved in the innervation of bladder function in the adult rat brainstem and spinal cord using pseudorabies virus，a transsynaptic tracer，so as to lay down the basis for further studying of brain functional reorganization after bladder function reconstruction. Methods　GFP-PRV 4.5ul（1×108 PFU /ml）was injected into the bladder wall of 15 adult female SD rats at 3 different sites. The distribution of virus-infected neurons in the brainstem, spinal cord and dorsal root ganglion were observed under fluorescent microscope at various intervals (72h, 84h, 96h, 108h, 120h) following the PRV injection.　Results　Fluorescence positive neurons were mainly present in L6~S1, L1~L2 dorsal root ganglion; sacral parasympathetic nucleus, intermediolateral cell column and dorsal commissural in L6~S1 and L1~L2 spinal cord segments; Barrington's nucleus, nucleus raphe magnus, the gigantocelluar reticular nucleus, the parapyramidal reticular formation, noradrenergic cell groups A5 and A7, locus coeruleus, periaqueductal gray and the ventral region of red nucleus in brainstem.　Conclusion　The structures labeled in spinal cord and brainstem are synaptically connected with the bladder and presumably involved in the neural control of the bladder.

Key words: Pseudorabies virus, Bladder, Micturition reflex pathways, Central nervous system

中图分类号:

盛珺, 肖燎原, 张月雷, 林浩东, 侯春林. 大鼠脊髓及脑干内膀胱支配中枢的跨突触示踪研究[J]. 中国临床解剖学杂志, 2013, 31(4): 435-440.

CHENG Jun, XIAO Liao-Yuan, ZHANG Ru-Lei, LIN Gao-Dong, HOU Chun-Lin. Transsynaptic tracing of CNS neural circuitry involved in the innervation of bladder function in the adult rat brainstem and spinal cord[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(4): 435-440.

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