LncRNA NEAT1对垂体瘤细胞系GH3侵袭和迁移的作用机制研究

doi:10.13418/j.issn.1001-165x.2023.5.08

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中国临床解剖学杂志 ›› 2023, Vol. 41 ›› Issue (5) : 543-549. DOI: 10.13418/j.issn.1001-165x.2023.5.08

实验研究

LncRNA NEAT1对垂体瘤细胞系GH3侵袭和迁移的作用机制研究

郑锴¹，罗秀玲²，张玮豪¹，刘宇利¹，李玉明¹，廖尚高³

作者信息 +

Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells

Zheng Kai ¹, Luo Xiuling², Zhang Weihao¹, Liu Yuli ¹, Li Yuming ¹, Liao Shanggao³

Author information +

文章历史 +

摘要

目的探讨lncRNA NEAT1对垂体瘤细胞侵袭、迁移的作用及机制。方法 qRT-PCR检测lncRNA NEAT1、miR-134和ITGB1表达；Transwell检测侵袭和迁移；双荧光素酶报告基因实验检测miR-134与lncRNA NEAT1、ITGB1的调控关系；Western blot检测ITGB1/FAK/PI3K通路相关蛋白表达。结果敲低lncRNA NEAT1抑制GH3细胞侵袭和迁移（P<0.05）；抑制miR-134可逆转下调lncRNA NEAT1对GH3细胞侵袭、迁移的抑制作用（P<0.05）。miR-134与lncRNA NEAT1和ITGB1存在负靶向调控关系（P<0.05）。上调miR-134抑制GH3细胞侵袭和迁移，抑制ITGB1/FAK/PI3K通路激活（P<0.05）；ITGB1过表达削弱上调miR-134对GH3细胞的影响（P<0.05）。结论下调lncRNA NEAT1靶向miR-134可能通过抑制ITGB1/FAK/PI3K通路激活抑制垂体瘤细胞侵袭、迁移。

Abstract

Objective To explore the effect and potential mechanism of lncRNA NEAT1 on the invasion and migration of pituitary tumor cells. Methods The expressions of lncRNA NEAT1, miR-134, and ITGB1 were detected by qRT-PCR. Transwell was used to detect the invasion and migration. The regulatory relationship between miR-134 and lncRNA NEAT1 and ITGB1 was detected by a double luciferase reporter gene experiment. The expression of ITGB1/FAK/PI3K pathway-related proteins was detected by Western blot. Results Knockdown of lncRNA NEAT1 inhibited the invasion and migration of GH3 cells (P<0.05); inhibition of miR-134 could reverse the inhibitory effect of down-regulated lncRNA NEAT1 on the invasion and migration of GH3 cells (P<0.05). miR-134 had a negative targeted regulation relationship with lncRNAs NEAT1 and ITGB1 (P<0.05). Up-regulation of miR-134 inhibited the invasion and migration of GH3 cells and inhibited the activation of the ITGB1/FAK/PI3K pathway (P<0.05). ITGB1 overexpression attenuated the effect of up-regulated miR-134 on GH3 cells (P<0.05). Conclusions Down-regulation of lncRNA NEAT1 targeting miR-134 may inhibit the invasion and migration of pituitary tumor cells by inhibiting the activation of the ITGB1/FAK/PI3K pathway.

导出引用

郑锴, 罗秀玲, 张玮豪, 刘宇利, 李玉明, 廖尚高. LncRNA NEAT1对垂体瘤细胞系GH3侵袭和迁移的作用机制研究[J]. 中国临床解剖学杂志. 2023, 41(5): 543-549 https://doi.org/10.13418/j.issn.1001-165x.2023.5.08

Zheng Kai, Luo Xiuling, Zhang Weihao, Liu Yuli, Li Yuming, Liao Shanggao. Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells[J]. Chinese Journal of Clinical Anatomy. 2023, 41(5): 543-549 https://doi.org/10.13418/j.issn.1001-165x.2023.5.08

中图分类号： R736.4

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