Macamide B促进缺氧缺血性脑损伤新生小鼠神经元的PGRN表达

doi:10.13418/j.issn.1001-165x.2023.3.10

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中国临床解剖学杂志 ›› 2023, Vol. 41 ›› Issue (3) : 304-309. DOI: 10.13418/j.issn.1001-165x.2023.3.10

实验研究

Macamide B促进缺氧缺血性脑损伤新生小鼠神经元的PGRN表达

周倩¹，李莉霞¹，杨晓夏¹，杨俊华¹，文锦坤¹，李朋飞²，罗利¹，李国营¹*

作者信息 +

Macamide B promoting the expression of PGRN in hypoxic-ischemic brain damage neonatal mice

Zhou Qian¹, Li Lixia¹, Yang Xiaoxia¹, Yang Junhua¹, Wen Jinkun¹, Li Pengfei², Luo Li¹, Li Guoying¹*

Author information +

文章历史 +

摘要

目的研究玛咖酰胺B（Macamide B）对缺氧缺血性脑损伤（hypoxic-ischemic brain damage，HIBD）的新生小鼠神经元中颗粒蛋白前体（Progranulin，PGRN）及糖氧剥夺（oxygen-glucose deprivation，OGD）后的小鼠海马神经元细胞系（HT22）中PGRN表达的影响，探究Macamide B在新生小鼠HIBD后发挥神经保护作用的机制。方法建立HIBD小鼠模型，将出生后7 d的C57小鼠随机分为Sham组、HIBD组和Macamide B组；HT22细胞培养并制备OGD细胞模型，分为Control组、OGD组和Macamide B组。通过免疫荧光、Western blot检测小鼠神经元或HT22细胞中PGRN的表达。结果体内实验显示，与Sham组相比，HIBD组小鼠神经元中PGRN表达降低；与HIBD组相比，Macamide B组小鼠神经元中PGRN表达升高。在体外，与Control组相比，OGD组HT22细胞中PGRN表达降低；与OGD组相比，Macamide B组HT22细胞中PGRN表达显著升高。结论 Macamide B可能通过促进PGRN表达对HIBD新生小鼠发挥神经保护作用。

Abstract

Objective To investigate the effect of Macamide B on the expression of progranulin (PGRN) in neurons of neonatal mice with hypoxic-ischemic brain damage (HIBD) and HT22 mouse hippocampal neuron cell line after oxygen-glucose deprivation (OGD) and explore the mechanism of the neuroprotective effect of Macamide B after HIBD in neonatal mice. Methods The HIBD model of C57 mice was established after 7 days of birth and the mice were divided into Sham group, HIBD group and Macamide B group. HT22 cells were cultured, then the OGD model of HT22 cells was established, and the cells were divided into Control group, OGD group and Macamide B group. The expression of PGRN in mouse neurons or HT22 cells was detected by immunofluorescence staining and Western blot. Results In vivo experiments showed that compared with Sham group, the expression of PGRN in mice neurons of HIBD group was reduced. Compared with HIBD group, the expression of PGRN in neurons of Macamide B group was increased. In vitro, compared with Control group, the expression of PGRN in HT22 cells of OGD group was decreased. Compared with OGD group, the expression of PGRN in HT22 cells of Macamide B group rose significantly. Conclusions Macamide B may play a neuroprotective role in HIBD neonatal mice by promoting the expression of PGRN.

导出引用

周倩, 李莉霞, 杨晓夏, 杨俊华, 文锦坤, 李朋飞, 罗利, 李国营. Macamide B促进缺氧缺血性脑损伤新生小鼠神经元的PGRN表达[J]. 中国临床解剖学杂志. 2023, 41(3): 304-309 https://doi.org/10.13418/j.issn.1001-165x.2023.3.10

Zhou Qian, Li Lixia, Yang Xiaoxia, Yang Junhua, Wen Jinkun, Li Pengfei, Luo Li, Li Guoying. Macamide B promoting the expression of PGRN in hypoxic-ischemic brain damage neonatal mice [J]. Chinese Journal of Clinical Anatomy. 2023, 41(3): 304-309 https://doi.org/10.13418/j.issn.1001-165x.2023.3.10

中图分类号： R722.1

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