模拟高原低氧小鼠学习记忆能力与肠道菌群结构改变的相关性研究

doi:10.13418/j.issn.1001-165x.2023.3.09

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中国临床解剖学杂志 ›› 2023, Vol. 41 ›› Issue (3) : 296-303. DOI: 10.13418/j.issn.1001-165x.2023.3.09

实验研究

模拟高原低氧小鼠学习记忆能力与肠道菌群结构改变的相关性研究

李文豪^1,2，齐宝宁¹，施艺²，王昱昊³，赵再华²，王瑜^1,2，沈学锋²*

作者信息 +

Correlation between learning and memory ability and gut microbiota structure in mice induced by high-altitude hypoxia

Li Wenhao^1,2, Qi Baoning¹, Shi Yi², Wang Yuhao³, Zhao Zaihua², Wang Yu^1,2, Shen Xuefeng²*

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文章历史 +

摘要

目的探讨小鼠在高原低氧环境中学习记忆能力和肠道菌群的特征性变化及其相关性。方法将C57BL/6小鼠随机分为对照组和低氧组，每组7只，低氧组模拟海拔4000米环境暴露4周。旷场实验检测小鼠自主活动能力及焦虑抑郁样情绪，新物体识别和水迷宫检测学习记忆能力，16S rDNA检测小鼠肠道菌群结构变化，Spearman相关系数分析认知功能与肠道菌群的相关性。结果与对照组相比，低氧组小鼠新物体识别辨别指数下降（P<0.05）；水迷宫目标象限停留的时间百分比降低（P<0.05），提示高原低氧暴露后小鼠学习记忆能力下降；16S rDNA结果显示肠道菌群结构改变，多样性下降，艾克曼菌属、另枝菌属、肠杆菌属和瘤胃球菌属相对丰度增加；Spearman相关性分析发现，辨别指数、目标象限停留的时间百分比与α多样性呈正相关（P<0.05），辨别指数与艾克曼菌属、另枝菌属的相对丰度呈负相关（R= -0.709、-0.604，P<0.05）；目标象限停留的时间百分比与艾克曼菌属、另枝菌属和瘤胃球菌属的相对丰度呈负相关（R= -0.704、-0.560、-0.547，P<0.05）。结论高原低氧暴露导致小鼠肠道菌群结构改变。其中，艾克曼菌属、另枝菌属和瘤胃球菌属相对丰度增加与小鼠学习记忆能力下降存在相关性。

Abstract

Objective To investigate the characteristic changes of learning and memory function, gut microbiota and their correlation in high-altitude hypoxia. Methods C57BL/6 mice were randomly divided into a control group and a hypoxia group, with 7 mice in each group. The hypoxia group simulated at an altitude of 4000 meters exposure for 4 weeks. The open field test, the novel object recognition and Morris water maze were used to detect the autonomous activity, anxiety- and depression-like and the learning and memory ability of the mice. 16S rDNA and Spearman correlation coefficient were used to detect the structural changes of gut microbiota and analyze the correlation between cognition and gut microbiota of the mice. Results Compared with the control group, the discrimination index and the percentage of time in the target quadrant were decreased significantly in hypoxia group (P<0.05). The structure of gut microbiota changed and the diversity decreased. The relative abundance of Akkermansia, Alistipes, Enterorhabdus and Ruminococcus increased at the genus level. The discrimination index and the percentage of time in the target quadrant were significantly positively correlated with α diversity (P<0.05), The discrimination index was negatively correlated with the relative abundance of Akkermansia and Alistipes (R= -0.709, -0.604, P<0.05). The percentage of time in the target quadrant was negatively correlated with the relative abundance of Akkermansia, Alistipes, and Ruminococcus (R= -0.704, -0.560, -0.547, P<0.05). Conclusions The exposure of high-altitude hypoxia causes the structural changes of intestinal flora in mice. The increased relative abundance of Akkermansia, Alistipes and Ruminococcus is correlated with the decreased learning and memory ability in mice.

导出引用

李文豪, 齐宝宁, 施艺, 王昱昊, 赵再华, 王瑜, 沈学锋. 模拟高原低氧小鼠学习记忆能力与肠道菌群结构改变的相关性研究[J]. 中国临床解剖学杂志. 2023, 41(3): 296-303 https://doi.org/10.13418/j.issn.1001-165x.2023.3.09

Li Wenhao, Qi Baoning, Shi Yi, Wang Yuhao, Zhao Zaihua, Wang Yu, Shen Xuefeng. Correlation between learning and memory ability and gut microbiota structure in mice induced by high-altitude hypoxia[J]. Chinese Journal of Clinical Anatomy. 2023, 41(3): 296-303 https://doi.org/10.13418/j.issn.1001-165x.2023.3.09

中图分类号： R338.64 R364.4 R339.5

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