基于Nrf2通路探讨松脂醇二葡萄糖苷改善小鼠骨质疏松的机制研究

doi:10.13418/j.issn.1001-165x.2023.2.08

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中国临床解剖学杂志 ›› 2023, Vol. 41 ›› Issue (2) : 162-171. DOI: 10.13418/j.issn.1001-165x.2023.2.08

实验研究

基于Nrf2通路探讨松脂醇二葡萄糖苷改善小鼠骨质疏松的机制研究

黄星翔¹，钟超²*，叶华²，徐春贻³，刘云飞⁴，蒋璐⁴

作者信息 +

Action mechanism of pinoresinol diglucoside on improving osteoporosis in mice based on Nrf2 pathway

Huang Xingxiang¹, Zhong Chao²*, Ye Hua², Xu Chunyi³, Liu Yunfei⁴, Jiang Lu⁴

Author information +

文章历史 +

摘要

目的分析松脂醇二葡萄糖苷（Pinoresinol diglucoside，PD）改善骨质疏松的作用机制。方法微型CT分析小鼠小梁骨体积/总体积（bone volume/total volume，BV/TV）、平均小梁厚度（average trabecular thickness，Tb.Th）、平均小梁数目（average number of trabeculae，Tb.N）和平均小梁间距（average trabecular spacing，Tb.Sp）；HE染色检测骨组织损伤；TRAP染色分析成骨细胞数量/骨周长（number of bone cells/bone circumference，N.Ob/B.Pm）、空泡率以及破骨细胞表面/骨表面百分比（osteoclast surface/bone surface，Ocs/BS）；ELISA检测碱性磷酸酶（alkaline phosphatase，ALP）、抗酒石酸酸性磷酸酶（tartrate-resistant acid phosphatase，TRAP）相对活性，超氧化物歧化酶（Superoxide dismutase，SOD）、丙二醛（Malondialdehyde，MDA）和谷胱甘肽（glutathione，GSH）含量；Wb检测核转录因子红细胞系2相关因子2（Nuclear factor erythroid-2-related factor 2，Nrf2）和HO-1表达。结果与假手术组相比，模型组小鼠骨丢失量增加，BV/TV、Tb.Th、Tb.N、N.Ob/B.Pm、ALP相对活性、SOD、GSH、Nrf2和HO-1表达水平明显下降，Tb.Sp、空泡率、Ocs/BS、TRAP相对活性和MDA水平明显上升（P<0.05）；E2组和PD组小鼠与模型组小鼠变化趋势完全相反（P<0.05），PD组和E2组小鼠上述指标无显著差异（P>0.05）。结论 PD可通过激活Nrf2信号通路改善小鼠骨质疏松症状。

Abstract

Objective To analyze the action mechanism of pinoresinol diglucoside (PD) on improving osteoporosis. Methods The trabecular bone volume/total volume (BV/TV), average trabecular thickness (Tb.Th), average number of trabeculae (Tb.N) and average trabecular spacing (Tb.Sp) were analyzed by micro-CT. The damage of bone tissues was detected by HE staining. Number of bone cells/bone circumference (N.Ob/B.Pm), vacuole rate and osteoclast surface/bone surface (Ocs/BS) were analyzed by TRAP staining. The relative activities of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were detected by ELISA. The expressions of Nuclear factor erythroid-2-related factor 2 (Nrf2) and HO-1 were detected by Western Blotting. Results Compared with sham operation group, bone loss was increased in model group, BV/TV, Tb.Th, Tb.N, N.Ob/B.Pm, relative activity of ALP, SOD, GSH, Nrf2 and HO-1 expression levels were significantly decreased, Tb.Sp, vacuole rate, Ocs/BS, relative activity of TRAP and MDA level were significantly increased (P<0.05). The changes in E2 group and PD group were completely opposite to those in model group (P<0.05). There was no significant difference in the above indexes between PD group and E2 group (P>0.05). Conclusions PD can improve osteoporosis in mice by activating Nrf2 signaling pathway.

导出引用

黄星翔, 钟超, 叶华, 徐春贻, 刘云飞, 蒋璐. 基于Nrf2通路探讨松脂醇二葡萄糖苷改善小鼠骨质疏松的机制研究[J]. 中国临床解剖学杂志. 2023, 41(2): 162-171 https://doi.org/10.13418/j.issn.1001-165x.2023.2.08

Huang Xingxiang, Zhong Chao, Ye Hua, Xu Chunyi, Liu Yunfei, Jiang Lu. Action mechanism of pinoresinol diglucoside on improving osteoporosis in mice based on Nrf2 pathway[J]. Chinese Journal of Clinical Anatomy. 2023, 41(2): 162-171 https://doi.org/10.13418/j.issn.1001-165x.2023.2.08

中图分类号： R33

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