目的 探讨白藜芦醇（resveratrol，RES）对腔隙性脑梗死（lacunar infarction，LI）大鼠脑组织损伤的影响。 方法 选取60只SD大鼠均分为假手术组、模型组、RES组以及联合组（RES+LY294002），月桂酸钠法制备LI模型，Zea-Longa’s法评估神经功能，TTC法观察脑梗死体积，酶联免疫法检测脑组织氧化应激、炎性因子水平，Weidener法观察微血管密度，Western blotting检测脑组织磷脂酰肌醇3-激酶/丝氨酸苏氨酸激酶（P13K/Akt）通路相关蛋白及血管内皮生长因子（VEGF）蛋白水平。 结果 RES组能降低LI大鼠神经功能评分、脑梗死体积和MDA、TNF-α、IL-6、IL-1β水平（P<0.05），增加微血管密度、SOD、CAT、GSH-PX、IL-10水平和VEGF、p-PI3K、p-AKT蛋白表达（P<0.05），联合组上述指标与RES组相比呈相反趋势（P<0.05）。 结论 RES可改善LI大鼠脑损伤和血管新生，PI3K/Akt信号通路参与该过程。
Abstract
Objective To explore the effects of resveratrol (RES) on brain tissue damage in rats with lacunar infarction (LI). Methods Sixty SD rats were divided into a sham operation group, a model group, a RES group and a combination group (RES+LY294002) averagely. LI models were prepared by sodium laurate method. The nerve function was evaluated by Zea-Longa's method. The cerebral infarction volume was observed by TTC. The levels of oxidative stress and inflammatory factors in brain tissues were detected by enzyme linked immunosorbent assay. The microvessel density was observed by Weidener method. The levels of phosphateidylinositol 3 kinase/serine-threonine kinase (PI3K/Akt) signaling pathways related proteins and vascular endothelial growth factor (VEGF) in brain tissues were detected by Western blotting. Results RES could reduce neurological scores, cerebral infarction volume and levels of MDA, TNF-α, IL-6 and IL-1β in LI rats (P<0.05), increase microvessel density, levels of SOD, CAT, GSH-PX and IL-10, and expressions of VEGF, p-PI3K and p-AKT proteins (P<0.05). Compared with the RES group, the above indexes in combination group showed an opposite trend (P<0.05). Conclusions RES can improve brain damage and angiogenesis in LI rats, and PI3K/Akt signaling pathways participate in this process.
关键词
腔隙性脑梗死 /
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白藜芦醇 /
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PI3K/Akt信号通路 /
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血管新生
Key words
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Lacunar infarction /
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Resveratrol /
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PI3K/Akt signaling pathway /
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Angiogenesis
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