目的 探究Hippo信号通路核心蛋白在小鼠出生后不同时间点皮质中的表达变化。方法 在C57BL/6小鼠出生后（postnatal，P）3 d、6 d、9 d、3周和8周，通过测量脑重及脑横径来检测小鼠脑组织生长发育情况；通过Western Blot检测小鼠皮质中MST1、LATS1的表达；通过酪酰胺信号放大技术检测小鼠皮质中小胶质细胞在小鼠出生后五个不同时间点的数目变化以及Yes-相关蛋白（Yes-associated protein，YAP）在小胶质细胞中的定位情况。 结果 小鼠出生后早期脑重、脑横径增速显著；MST1和LATS1从P9d开始极显著降低，P3周与P8周相比无统计学意义；皮质中IBA1阳性小胶质细胞数目占比随鼠龄上升，出生后早期增速显著，伴随小胶质细胞数目增多的是YAP从核外转向核内聚集。 结论 Hippo信号通路在出生后早期的小鼠皮质中被激活，可能参与出生后脑发育时体积调控。
Abstract
Objective To explore the expression changes of Hippo signaling pathway core protein at different time points of postnatal mice cortex. Methods Brain weight and transverse diameter were measured at 3 d, 6 d, 9 d, 3w and 8w postnatal (P) mice cortex of C57BL/6 to detect the growth and development of mice brain tissue. Western Blot was used to detect the expression of MST1 and LATS1. Tyramide Signal Amplification (TSA) was used to detect the changes of microglia numbers and the location of Yes-associated protein (YAP) in microglia at five different time points. Results The brain weight and transverse diameter in the early postnatal period was significantly increased. Western blot result showed that MST1 and LATS1 were significantly decreased from P9d, and there was no statistical difference in P3w and P8w. The proportion of IBA1 positive microglia in cortex were increased with the increasing of age, with a significant increase in the early postnatal period. With the increasing number of microglia, YAP aggregation transferred from extranuclear to intranuclear. Conclusions Hippo signaling pathway is activated in early postnatal mice cortex, which may be involved in volume regulation during postnatal brain development.
关键词
Hippo信号通路； /
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小胶质细胞； /
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C57BL/6小鼠
Key words
Hippo signaling pathway; /
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Microglia; /
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C57BL/6 mice
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基金
国家自然科学基金（81860246）；广西科技基地和人才专项（桂科AD18281011）；广西自然科学基金 ( 2018GXNSFAA281105)
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