目的 基于网络药理学分析“络石藤-牛膝”药对治疗关节炎（Arthritis）的潜在作用机制。 方法 通过检索中草药系统药理学数据库平台（TCMSP）进行药对“络石藤-牛膝”有效成分及靶基因筛选；以“Arthritis”为关键词，在GeneCards数据库、NCBI基因数据库以及OMIM数据库进行人类基因检索并获得关节炎相关基因；将筛选出的药对靶点与疾病靶点输入韦恩图制作软件Venny2.1，获得共有靶点；运用STRING数据库进行PPI网络构建，Cystoscape构建“药物-成分-靶点-疾病”网络；运用R语言软件对关键靶基因进行基因本体论（GO）功能富集分析及与基因组百科全书（KEGG）通路的集分析。 结果 从TCMSP中筛选获得络石藤9种化合物成分，94个靶点；牛膝获得21种化合物成分，205个靶点。Venny映射得到128个共有靶点，STRING数据库筛选出5个基因簇和4个核心基因构建PPI网络。GO功能富集分析显示，“络石藤-牛膝”药对涉及2329条生物过程，150项分子功能相关，60项细胞组成相关。通过KEGG通路富集分析，“络石藤-牛膝”药对治疗关节炎富集到154条信号通路。 结论 基于网络药理学，从有效成分、基因靶点、信号通路等方面初步验证了“络石藤-牛膝”药对治疗关节炎的作用机制。
Abstract
Objective To analyze the potential mechanism of herb-pair “Trachelospermum jasminoides and Achyranthes bidentata” based on network pharmacology in Arthritis. Methods The effective components and target genes of “Trachelospermum jasminoides and Achyranthes bidentata” were screened by searching the pharmacological database platform of Chinese herbal medicine system (TCMSP). Setting “Arthritis” as the key word, human gene retrieval was performed in GeneCards database, NCBI gene database and OMIM database to obtain genes, which were related to Arthritis. The selected drug pair targets and disease targets were input into Venny 2.1 software to obtain common targets. The STRING database was used to construct PPI network, and the “herb-component-target-disease” network was constructed by Cytoscape. The gene ontology (GO) functional enrichment analysis and KEGG pathway analysis of key target genes were carried out by R language software. Results Totally, 9 compounds and 94 targets of Trachelospermum jasminoides were obtained from TCMSP, while 21 compounds and 205 targets got from Achyranthes bidentata. Venny mapping obtained 128 common targets, and a total of 5 gene clusters and 4 core genes were screened out from the STRING database to construct PPI network. The GO functional enrichment analysis showed that the herb-pair “Trachelospermum jasminoides and Achyranthes bidentata” involved 2329 biological processes, 150 molecular function correlations and 60 cell composition correlations. Through KEGG pathway enrichment analysis, “Trachelospermum jasminoides and Achyranthes bidentata” herb-pair in the treatment of Arthritis enriched a total of 154 signal pathways. Conclusions Based on network pharmacology, the possible mechanism of herb-pair “Trachelospermum jasminoides and Achyranthes bidentata” in the treatment of Arthritis was preliminarily verified from the aspects of active components, gene targets and signal pathways.
关键词
络石藤； /
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药对； /
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牛膝； /
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关节炎； /
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网络药理学
Key words
Trachelospermum jasminoides; /
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Herb-pair; /
Achyranthes bidentata; /
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Arthritis; /
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Network pharmacology
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基金
国家自然科学基金（81360524，81260673）；广西地市县立项科研项目（Z202016）；全国中药特色技术传承人才培训项目(2015481601003)；2022年度广西高校中青年教师科研基础能力提升项目(2022ky1206)；自治区中医药局自筹经费科研课题（GZZC2019005）
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