目的 通过对胶质瘤公共数据库中CBX3表达量的分析，明确其与胶质瘤预后的相关性。流式细胞术以及对凋亡指标的检测分析其对胶质瘤细胞系凋亡水平的影响。 方法 通过对TCGA以及CGGA中胶质瘤患者基因芯片的数据进行分析，比较CBX3在不同级别胶质瘤中的表达量以及分析CBX3表达量与胶质瘤患者预后之间的关系。GO和Pathway分析对CBX3基因进行功能注释。最后在细胞系中探索CBX3基因对胶质瘤细胞系凋亡水平的影响。 结果 高级别胶质瘤中CBX3表达量明显高于低级别的胶质瘤中CBX3的表达量(P<0.01) 。高表达CBX3的患者预后比低表达CBX3的患者更差( P<0.05)。同时流式细胞术实验发现过表达CBX3可以显著降低胶质母细胞瘤细胞系U87MG和T98G的凋亡水平。 结论 CBX3的表达水平与胶质瘤的生存期呈负相关关系，高表达 CBX3可以降低胶质母细胞瘤细胞系的凋亡水平，故而CBX3或许可作为判断胶质母细胞瘤预后的有效指标。
Abstract
Objective To clarify the correlation between CBX3 and the prognosis of glioma by analyzing the expression of CBX3 in the glioma public database. Flow cytometry and the detection of apoptosis indexes were used to analyze its effect on the apoptosis level of glioma cell lines. Methods By analyzing the data of glioma patients gene chip in TCGA and CGGA, the expression of CBX3 in different grades of glioma was compared and the relationship between CBX3 expression and prognosis of glioma patients was analyzed. Functional annotation of the CBX3 gene by GO and Pathway analysis. Finally, the effect of CBX3 gene on the apoptosis level of glioma cell lines was explored in cell lines. Results The expression of CBX3 in high-grade gliomas was significantly higher than that in low-grade gliomas (P<0.01). The prognosis of patients with high CBX3 expression was worse than that of patients with low CBX3 expression (P<0.05). At the same time, flow cytometry experiments showed that overexpression of CBX3 could significantly reduce the apoptosis level of glioblastoma cell lines U87MG and T98G. Conclusion The expression level of CBX3 is negatively correlated with the survival time of glioma. High expression of CBX3 can reduce the apoptosis level of glioblastoma cell lines, so CBX3 may be an effective indicator for judging the prognosis of glioblastoma.
关键词
胶质母细胞瘤； /
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CBX3； /
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生物信息学； /
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预后
Key words
Glioblastoma; /
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CBX3; /
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Bioinformatics analysis; /
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Prognosis
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基金
国家自然科学基金(81802830，81902887，81972355)；广东省自然科学基金（2019B151502048）
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