目的 观察生骨再造丸对激素性股骨头坏死（steroid-induced osteonecrosis of the femoral head，SONFH）兔模型股骨头组织形态学及骨计量学相关指标的干预作用。 方法 将20只新西兰兔随机均分为正常组、模型组、生骨再造丸组和阳性对照药物仙灵骨葆胶囊组，除正常组外其余3组进行内毒素联合甲泼尼龙注射液造模。28 d后，生骨再造丸组以2.4 g/kg生骨再造丸灌胃，仙灵骨葆胶囊组以0.4 g/kg仙灵骨葆胶囊灌胃，正常组和模型组以10 mL生理盐水灌胃，1次/d。28 d后取各组兔双侧股骨头，Micro-CT扫描并进行骨计量学参数分析，HE染色观察股骨头组织病理学改变。 结果 与正常组比较，Micro-CT扫描分析显示模型组兔股骨头骨密度、骨体积分数、骨小梁数量、骨小梁厚度和骨小梁连接密度等骨计量学参数明显降低，骨小梁分离度和结构模型指数升高（P<0.05）；HE染色观察发现模型组空骨陷窝率和脂肪细胞面积明显增加，骨骺变形，骨小梁增生、变窄或断裂。与模型组比较，生骨再造丸组和仙灵骨葆胶囊组兔股骨头的骨计量学和组织病理学改变明显好转（P<0.05），且生骨再造丸组与仙灵骨葆胶囊组疗效无显著差异（P>0.05）。 结论 生骨再造丸可显著提升兔SONFH骨计量学指标水平，改善组织病理形态，从而达到治疗SONFH的作用。
Abstract
Objective To observe the intervention effect of Shenggu Zaizao Pills on steroid-induced necrosis of the femoral head (SONFH) rabbit femoral head histomorphology and related indexes of osteometry. Methods Twenty female New Zealand rabbits were randomly divided into a normal group, a model group, a Shenggu Zaizao pill group and a positive control drug Xianling Gubao capsule group, 5 in each group. Except the normal group, the other three groups received endotoxin combined with methylprednisone sodium succinate injection to modeling. After 28d, Shenggu Zaizao Pills group was given 2.4 g/kg Shenggu Zaizai Pills, Xianling Gubao Capsule group was given 0.4 g/kg Xianling Gubao Capsule, normal group and model group were given 10mL of normal saline, 1 time/d. After 28d, the bilateral femoral heads of the rabbits in each group were taken to perform Micro-CT scanning to analyze osteometric parameters. HE staining was used to observe the pathological changes of the femoral head tissue morphology. Results Compared with the normal group, Micro-CT scan analysis showed that the femoral head bone mineral density (BMD),bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th) and connectivity density (Conn.D) in the model group significantly decreased, while trabecular bone separation (Tb.Sp) and structural model index (SMI) significantly increased (P<0.05). Microscopic observation of HE stained pathological sections showed that the rate of empty bone lacunae and the area of adipocytes in the model group significantly increased, the epiphysis was deformed, and the trabecular bone was hyperplasia, narrowed or broken. Compared with the model group, the osteometric and histopathological changes of the femoral head of the rabbits in the Shenggu Zaizao Pills group and Xianling Gubao Capsule group significantly improved (P<0.05). There was no significant difference in curative effect between Shenggu Zaizao Pills group and Xianling Gubao Capsules group (P>0.05). Conclusions Shenggu Zaizao Pills can significantly increase the level of SONFH osteometric indexes in rabbits and improve histopathological morphology, thereby achieving the effect of treating SONFH.
关键词
激素性股骨头坏死； /
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生骨再造丸； /
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骨组织形态； /
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骨计量； /
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兔模型
Key words
Steroid-induced femoral head necrosis; /
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Shenggu Zaizao Pills; /
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Bone tissue morphology /
Bone measurement; /
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Rabbit modeling
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基金
国家自然科学基金（81860859）；国家自然科学基金（82160915）；甘肃中医药大学研究生创新基金（2021CX02）
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