目的 研究p-mTOR、p-4EBP1及p-S6K1在结直肠腺癌发生、发展过程中可能的作用及临床意义。 方法 应用免疫组化检测60例结直肠腺癌组织及40例癌旁正常组织中p-mTOR、p-4EBP1及p-S6K1的表达情况，比较各组差异，分析其与相关临床病理特征的关系，阳性率间相关性分析采用Spearman相关分析检验，检验水准α=0.05。 结果 p-mTOR、p-4EBP1及p-S6K1在结直肠腺癌组织中表达水平均高于癌旁组织，差异有统计学意义（P<0.05）。p-mTOR的表达与患者的淋巴结转移、TNM分期及分化程度有关（P<0.05）。p-S6K1的表达与患者的TNM分期及分化程度有关（P<0.05）。p-mTOR与p-4EBP1在结直肠腺癌组织中的表达有一定的正相关性（P<0.05）。p-mTOR与p-S6K1在结直肠腺癌组织中的表达有明显的正相关性（P<0.05）。 结论 p-mTOR、p-4EBP1及p-S6K1在结直肠腺癌粘膜组织中过度表达可能是结直肠腺癌发生的重要机制之一，此三种磷酸化蛋白与结直肠腺癌的发生发展存在某种内在关联。
Abstract
Objective To study the expression of p-mTOR, p-4EBP1 and p-S6K1 in colorectal adenocarcinoma and adjacent tissues and analyze their relationship with relevant clinicopathological data, and explore their occurrence and development in colorectal adenocarcinoma and the possible role and clinical significance. Methods Immunohistochemistry was used to detect the expression of p-mTOR, p-4EBP1 and p-S6K1 in 60 cases of colorectal adenocarcinoma tissues and 40 cases of adjacent normal tissues, compare the differences of each group, and analyze their relationship with relevant clinicopathological characteristics. The positive rate correlation analysis was performed by Spearman correlation analysis, and the test level α=0.05. Results The expression levels of p-mTOR, p-4EBP1 and p-S6K1 in colorectal adenocarcinoma tissues were higher than those in adjacent tissues on average, with statistically significant differences (P<0.05). The expression of p-mTOR was related to the stage of patients with lymph node metastasis, TNM stage and differentiation degree (P<0.05). The expression of p-S6K1 was related to the TNM stage and differentiation degree of the patient (P<0.05). The expression of p-mTOR and p-4EBP1 in colorectal adenocarcinoma tissue has a certain positive correlation (P<0.05), and the expression of p-mTOR and p-S6K1 in colorectal adenocarcinoma tissue has a significant positive correlation (P<0.05). Conclusions The overexpression of p-mTOR, p-4EBP1 and p-S6K1 in the mucosal tissue of colorectal adenocarcinoma may be one of the important mechanisms for the occurrence of colorectal adenocarcinoma. These three phosphorylated proteins may be related to the occurrence and development of colorectal adenocarcinoma.
关键词
结直肠腺癌； /
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p-mTOR； /
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p-4EBP1； /
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p-S6K1； /
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免疫组化
Key words
Nasopharyngeal carcinoma; /
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Drug resistance; /
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Micro RNA; /
Cisplatin; /
Cyclin protein
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基金
国家自然科学基金面上项目（81773247）
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