目的 研究细胞骨架相关蛋白PDLIM5在人成纤维细胞内的定位及其调控作用。 方法 通过慢病毒转染下调PDLIM5在人成纤维细胞中的表达后，进行CCK8细胞增殖实验、细胞划痕实验、Transwell迁移实验来检测PDLIM5对细胞增殖和运动迁移能力的影响，通过免疫荧光染色来观察PDLIM5的亚细胞定位。 结果 下调PDLIM5表达后抑制了人成纤维细胞的增殖、运动和迁移能力，免疫荧光发现PDLIM5主要表达在细胞质并定位于微丝。 结论 PDLIM5定位于细胞骨架微丝，下调PDLIM5表达可抑制人成纤维细胞的增殖、运动和迁移能力。
Abstract
Objective To investigate the localization and regulation of cytoskeleton-related proteins PDLIM5 in human fibroblasts． Methods After the expression of PDLIM5 was down-regulated by lentivirus transfection in human fibroblasts, CCK8 cell proliferation assay, cell scratch assay and Transwell migration assay were performed to detect the effect of PDLIM5 on cell proliferation and migration ability. Immunofluorescence staining was used to observe the cellular localization of PDLIM5. Results Down-regulating of PDLIM5 expression inhibited, the proliferation and migration ability of human fibroblasts. Immunofluorescence staining showed that PDLIM5 was mainly expressed in the cytoplasm and localized in the microfilament. Conclusions PDLIM5 is localized in cytoskeleton microfilaments and down-regulation of PDLIM5 expression can inhibit cell proliferation and migration ability of human fibroblasts.
关键词
人成纤维细胞； /
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PDLIM5； /
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微丝； /
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细胞骨架相关蛋白
Key words
Human fibroblasts; /
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PDLIM5; /
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Microfilaments; /
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Cytoskeleton associated proteins
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参考文献
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基金
国家重点研发计划(2017YFC1105000); 深圳市医疗卫生三名工程(SZSM201612019)
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