长链非编码LncRNA-MEG3在激素性股骨头坏死中的表达及相关性研究

doi:10.13418/j.issn.1001-165x.2022.2.13

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中国临床解剖学杂志 ›› 2022, Vol. 40 ›› Issue (2) : 187-191. DOI: 10.13418/j.issn.1001-165x.2022.2.13

实验研究

长链非编码LncRNA-MEG3在激素性股骨头坏死中的表达及相关性研究

蔡昊¹，陈春²*

作者信息 +

Expression and correlation of long non-coding lncRNA-MEG3 in steroid-induced femoral head necrosis

Cai Hao¹, Chen Chun²*

Author information +

文章历史 +

摘要

目的探讨长链非编码RNA-MEG3（lncRNA-MEG3）在激素性股骨头坏死中的表达及意义。方法应用实时荧光定量PCR方法检测股骨颈骨折患者股骨头组织（30例）和激素性股骨头坏死患者的股骨头及其旁组织（30对）中LncRNA-MEG3的表达并分析其临床意义。结果激素性坏死的股骨头骨组织中lncRNA-MEG3的含量最高，其次为其旁组织，股骨颈骨折患者骨组织中含量最低，差异均有统计学意义（P<0.05）；两组间患者的性别比例、年龄（<60岁或≥60岁）比例无显著性差异（P>0.05）；股骨头坏死组，服用不同种类激素（甲泼尼龙、地塞米松）的患者间lncRNA-MEG3含量无显著差别（P>0.05）；在不同ARCO分期（III期或IV期）患者中，IV期lncRNA-MEG3相对表达量明显高于III期（P<0.05）。结论长链非编码RNA-MEG3的表达与激素性股骨头坏死关系密切，且它的表达与激素性股骨头坏死的ARCO分期相关。

Abstract

Objective To investigate the expression and significance of long non-coding RNA-MEG3(lncRNA-MEG3) in steroid-induced femoral head necrosis. Methods Real-time fluorescence quantitative PCR was used to detect the expression of lncRNA-MEG3 in femoral head tissues of patients with femoral neck fracture (30 cases) and femoral head and its adjacent tissues of patients with steroid-induced femoral head necrosis (30 pairs), and its clinical significance was analyzed. Results The content of lncRNA-MEG3 was the highest in the femoral head bone tissues with steroid-induced necrosis, its adjacent tissues was followed, and the lowest was the bone tissues of patients with femoral neck fracture, the difference was statistically significant (P<0.05). There was no significant difference in the ratio of gender and age(less than 60 years old or more than 60 years old) between the two groups(P>0.05). In the femoral head necrosis group, there was no significant difference in lncRNA-MEG3 content among patients taking different hormone types (meprednisone or dexamethasone) (P>0.05). In patients with different ARCO stages (stage Ⅲ orⅣ), the relative expression of lncRNA-MEG3 in stage IV was higher that of stage Ⅲ (P<0.05). Conclusions The expression of long non-coding RNA-MEG3 is closely related to steroid-induced femoral head necrosis, and its expression is related to ARCO stage of steroid-induced femoral head necrosis.

导出引用

蔡昊, 陈春. 长链非编码LncRNA-MEG3在激素性股骨头坏死中的表达及相关性研究[J]. 中国临床解剖学杂志. 2022, 40(2): 187-191 https://doi.org/10.13418/j.issn.1001-165x.2022.2.13

Cai Hao, Chen Chun. Expression and correlation of long non-coding lncRNA-MEG3 in steroid-induced femoral head necrosis[J]. Chinese Journal of Clinical Anatomy. 2022, 40(2): 187-191 https://doi.org/10.13418/j.issn.1001-165x.2022.2.13

中图分类号： R681.8

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