山莨菪碱缓解幼龄鼠缺氧缺血性脑组织形态和功能损伤

doi:10.13418/j.issn.1001-165x.2021.05.011

中国临床解剖学杂志 ›› 2021, Vol. 39 ›› Issue (5): 557-562.doi: 10.13418/j.issn.1001-165x.2021.05.011

山莨菪碱缓解幼龄鼠缺氧缺血性脑组织形态和功能损伤

朱渝1，　魏静2，　吴鹏程1，　袁潇1，　周振华3，　李敏1

1.重庆市第七人民医院神经内科，重庆 400054； 2.重庆医科大学附属永川医院神经内科，重庆 402160；
3.陆军军医大学新桥医院神经内科，重庆 400038

收稿日期:2021-01-14 出版日期:2021-09-25 发布日期:2021-09-29
通讯作者: 李敏，副主任医师，E-mail：2568573271@qq.com
作者简介:朱渝（1972-），男，重庆人，硕士，副主任医师，E-mail：HPeveryDay2020@163.com
基金资助:
重庆市技术创新与应用发展专项重点项目（cstc2019 jscx-gksbX0064）

Anisodamine relieves hypoxic-ischemic brain damage and neurologic impairment in young rats

Zhu Yu 1, Wei Jing 2, Wu Pengcheng 1, Yuan Xiao 1, Zhou Zhenhua3, Li Min 1

1. Department of Neurology, Chongqing Seventh People's Hospital, Chongqing 400054, China; 2. Department of Neurology, Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing 402160, China; 3. Department of Neurology, Xinqiao Hospital, Army Military Medical University, Chongqing 400038, China

Received:2021-01-14 Online:2021-09-25 Published:2021-09-29

摘要/Abstract

摘要： 目的　探讨山茛菪碱在缺氧缺血性脑损伤幼龄鼠脑组织形态和功能损伤中的调控作用。方法　50只幼龄大鼠均分为健康对照组、模型组、模型加药组（尾静脉注射山莨菪碱2.5、5、10 mg/kg）共5组。脑组织干湿重法检测脑指数和脑含水率，HE染色观察脑组织病理损伤，TUNEL染色观察脑海马神经元周围组织细胞凋亡情况，Western blot检测脑组织中Bax/Bcl-2、caspase-9、caspase-3、BDNF和NGF蛋白表达水平，RT-PCR检测BDNF和NGF mRNA表达水平，试剂盒检测SOD、MDA和GSH-Px含量。结果　与健康对照组比较，模型组幼龄鼠脑组织形态和神经功能损伤严重（P<0.05）。与模型组比较，5 mg/kg和10 mg/kg山茛菪碱组脑指数和脑含水率降低（P<0.05），脑组织病理损伤好转，脑海马神经元周围组织细胞凋亡减少，Bax/Bcl-2、caspase-9、caspase-3表达水平降低（P<0.05），BDNF和NGF表达水平增高（P<0.05），MDA含量降低，SOD和GSH-Px含量增高（P<0.05）。结论　山茛菪碱能够缓解缺氧缺血性脑损伤幼龄鼠脑组织形态和功能损伤。

关键词: 山茛菪碱, 　缺氧缺血性脑损伤, 　幼龄鼠模型

Abstract: Objective　To investigate the regulatory effect of anisodamine on brain injury and neurological damage in young rats with hypoxic-ischemic brain damage.　Methods　Fifty young rats were divided into five groups (n=10): a healthy control group, a model group, and three model dosing groups (intravenously injected anisodamine for 2.5, 5, 10 mg/kg). Brain tissue wet and dry weight method was used to detect brain index and brain water content. HE staining was used to observe the pathological damage of brain tissue. TUNEL staining was used to observe the apoptosis of brain tissues around hippocampal neurons. Western blot detects the protein expression levels of Bax/Bcl-2, caspase-9, caspase-3, BDNF and NGF in brain tissue. RT-PCR was used to detect the expression level of BDNF and NGF mRNA, and the kit was used to detect the content of SOD, MDA and GSH-Px.　Results　Compared with the healthy control group, the brain tissue and nerve function of young rats in the model group were seriously damaged (P<0.05). Compared with the model group, the brain index and brain water content of young rats in the 5 mg/kg and 10 mg/kg anisodamine groups significantly reduced (P<0.05). The pathological damage of the brain tissue significantly improved, and the apoptotic cells around the hippocampal neurons in the brain Apoptosis decreased, Bax/Bcl-2, caspase-9, and caspase-3 expression levels decreased (P<0.05), BDNF and NGF expression levels increased (P<0.05), MDA content decreased, SOD and GSH-Px content increased (P<0.05).　Conclusions　Anisodamine can alleviate brain damage and nerve function damage in young rats with HIBD.

Key words: , Anisodamine, 　Hypoxic-ischemic brain damage, 　Young rats model

中图分类号:

R742

朱渝, 　魏静, 　吴鹏程, 　袁潇, 　周振华, 　李敏. 山莨菪碱缓解幼龄鼠缺氧缺血性脑组织形态和功能损伤[J]. 中国临床解剖学杂志, 2021, 39(5): 557-562.

Zhu Yu , Wei Jing , Wu Pengcheng , Yuan Xiao , Zhou Zhenhua, Li Min . Anisodamine relieves hypoxic-ischemic brain damage and neurologic impairment in young rats[J]. Chinese Journal of Clinical Anatomy, 2021, 39(5): 557-562.

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山莨菪碱缓解幼龄鼠缺氧缺血性脑组织形态和功能损伤

Anisodamine relieves hypoxic-ischemic brain damage and neurologic impairment in young rats

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