目的 观察瑞香素（daphnetin）即E003处理后RSA模型小鼠蜕膜组织形态结构的改变。 方法 建立反复自然流产（recurrent spontaneous abortion，RSA）模型小鼠后，实验分正常妊娠组、RSA模型组、E003低剂量组和E003高剂量组共4组，E003连续灌胃处理14 d后，取蜕膜组织。光镜下观察蜕膜组织血管和细胞形态，电镜下观察蜕膜组织超微结构。 结果 解剖子宫计算胚胎吸收率，E003处理组（低剂量组和高剂量组）低于RSA模型组，差异具有统计学意义，P<0.05；E003高剂量组胚胎吸收率与正常妊娠组相比无统计学差异，P>0.05。光镜下可见RSA模型组蜕膜细胞碎裂，数量较正常妊娠组减少；E003低剂量组和高剂量组蜕膜细胞数量均较模型组增多，排列较整齐。电镜下RSA模型组较正常妊娠组蜕膜细胞体积减小，细胞间隙增大，血管壁不完整，血管内皮细胞结构不完整，细胞核呈梭形；E003低剂量组与模型组比较，蜕膜细胞体积增大，细胞间隙增大，血管壁较完整，血管内皮细胞呈长梭形，细胞结构较完整，细胞间隙稍增宽；E003高剂量组蜕膜细胞组织及血管壁接近正常妊娠组。 结论 RSA模型小鼠存在母胎界面血管生成障碍，E003可修复RSA模型小鼠蜕膜组织中破坏的细胞结构，改善血管生成和细胞器形态，对RSA模型小鼠具有一定的治疗作用。
Abstract
Objective To observe the ultrastructural changes of decidual tissues in recurrent spontaneous abortion (RSA) model mice after treated with E003. Methods The RSA model mice was established, the experiment was divided into the following four groups: a normal pregnancy control group, a RSA model group, a low dose E003 group and a high dose E003 group. After 14 days of continuous gavage of E003, decidual tissues were taken. Light microscope was used to observe the vascular and cell morphology of decidual tissue, ultrastructure of decidual tissue. Results The uterus was dissected to calculate the absorption rate of the embryo. The results showed that the E003 treatment group (low-dose group and high-dose group) had lower embryo absorption rate than that of the RSA model group, and the difference was statistically significant (P<0.05).There was no significant difference in embryo absorption rate between the high-dose E003 group and the normal pregnancy group (P>0.05). Compared with the normal pregnancy group, the number of decidual cells in RSA model group significantly reduced. Compared with the model group, the decidua cells in the low dose group and the high dose group increased in number and were arranged neatly. Under electron microscope, the volume of deciduous cell in the RSA model group decreased compared with the normal pregnancy group, the space of cells was larger, vascular wall was incomplete, vascular endothelial cell structure was incomplete and the nucleus was fusiform. Compared with the model group, the intercellular space and volume of decidual cell volume in the E003 low-dose group increased. The vascular wall was more complete, the vascular endothelial cells were long fusiform, the vascular endothelial cell structure was more complete, and the intercellular space was slightly wider. The decidual cell tissue and blood vessel in the high-dose E003 group was close to the normal pregrancy group. Conclusions RSA model mice have the barrier of maternal-fetal interface angiogenesis, and E003 can repair the damaged cell structure in the decidua tissue of RSA model mice, improve the morphology of angiogenesis and organelles, which has a certain therapeutic effect on RSA model mice.
关键词
瑞香素 /
反复自然流产 /
透射电镜 /
蜕膜组织
Key words
Daphnetin; Recurrent spontaneous abortion; Transmission electron microscope; /
/
Decidual tissue
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参考文献
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基金
江西省自然科学基金（20171BAB215012）；江西省中医药管理局科技计划（2020B0318）
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