miR-146a通过靶向CCNJ调控鼻咽癌细胞HNE-1/DDP对顺铂敏感性的影响

doi:10.13418/j.issn.1001-165x.2021.01.011

中国临床解剖学杂志 ›› 2021, Vol. 39 ›› Issue (1) : 55-59. DOI: 10.13418/j.issn.1001-165x.2021.01.011

实验研究

miR-146a通过靶向CCNJ调控鼻咽癌细胞HNE-1/DDP对顺铂敏感性的影响

周兰柱，　赵报，　张明洁，　崔忆旋，　吴俊，　孙哲，　柳申成，　王文忠

作者信息 +

Effect of miR-146a on the sensitivity of nasopharyngeal carcinoma cells HNE-1 / DDP to cisplatin by targeting CCNJ

Zhou Lanzhu, Zhao Bao, Zhang Mingjie, Cui Yixuan, Wu Jun, Sun Zhe, Liu Shencheng, Wang Wenzhong

Author information +

文章历史 +

摘要

目的　探讨miR-146a对鼻咽癌耐顺铂细胞HNE-1/DDP顺铂敏感性的作用及可能机制。方法　实验分空白组、阴性对照组及mimics-miR-146a组。MTT检测HNE-1/DDP细胞在不同方法处理后的增殖抑制作用；Annexin V-FITC／PI双染法检测细胞凋亡情况；Western blot检测周期蛋白CCNJ、MRP1、P-gp的相对表达水平；实时荧光定量PCR（RT-qPCR）检测CCNJ mRNA的相对表达水平。结果　与其他两组相比，mimics-miR-146a组存活率降低，凋亡率升高，MRP1、P-gp、CCNJ蛋白表达量降低，CCNJ mRNA的相对表达量降低，差异均有统计学意义（P<0.05）。结论　miR-146a可增加HNE-1/DDP对DDP的敏感性，机制可能是miR-146a抑制CCNJ的表达而调控下游蛋白MRP1、P-gp，降低细胞耐药性。

Abstract

Objective　To investigate the effect of miR-146a on cisplatin sensitivity of nasopharyngeal carcinoma HNE-1 / DDP cells and its possible mechanism.　Methods　The experiment was divided into three groups: a blank control group, a negative control group and a mimics-miR-146a group. MTT assay was used to detect the proliferation inhibition of cells after different treatments. Annexin V-FITC / PI assay was used to detect apoptosis. Western blot was used to detect the relative expression levels of cyclin CCNJ, MRP1, P-gp. RT-qPCR was used to detect the relative expression level of CCNJ mRNA.　Results Compared with other two groups, the survival rate, the relative protein expression levels of MRP1, P-gp, CCNJ and the relative expression level of CCNJ mRNA of mimics-miR-146a reduced, while the apoptosis rate increased. There were statistical differences in the survival rate, the relative protein expression levels of MRP1, P-gp, CCNJ, the relative expression level of CCNJ mRNA and the apoptosis rate among the three groups (P<0.05).　Conclusions　miR-146a can increase the sensitivity of HNE-1/DDP to DDP which mechanism may be through miR-146a inhibiting the expression of CCNJ to regulate downstream proteins MRP1,P-gp,and then to reduce cell resistance.

导出引用

周兰柱, 赵报, 张明洁, 崔忆旋, 吴俊, 孙哲, 柳申成, 王文忠. miR-146a通过靶向CCNJ调控鼻咽癌细胞HNE-1/DDP对顺铂敏感性的影响[J]. 中国临床解剖学杂志. 2021, 39(1): 55-59 https://doi.org/10.13418/j.issn.1001-165x.2021.01.011

Zhou Lanzhu, Zhao Bao, Zhang Mingjie, Cui Yixuan, Wu Jun, Sun Zhe, Liu Shencheng, Wang Wenzhong. Effect of miR-146a on the sensitivity of nasopharyngeal carcinoma cells HNE-1 / DDP to cisplatin by targeting CCNJ[J]. Chinese Journal of Clinical Anatomy. 2021, 39(1): 55-59 https://doi.org/10.13418/j.issn.1001-165x.2021.01.011

中图分类号： R766.3

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