基于数字全息显微镜的人类精子的三维动态行为监测技术

doi:10.13418/j.issn.1001-165x.2020.04.021

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (4) : 476-480. DOI: 10.13418/j.issn.1001-165x.2020.04.021

临床研究

基于数字全息显微镜的人类精子的三维动态行为监测技术

孙丽¹，　黄桂²，　龚湘君²，　欧建平¹

作者信息 +

Digital holographic microscopy (DHM) and its application in monitoring three-dimensional dynamics of human spermatozoa

SUN Li¹, HUANG Gui², GONG Xiang-jun² , OU Jian-ping¹

Author information +

文章历史 +

摘要

目的　发展基于数字全息显微镜的人类精子三维动态行为的监测技术。方法　采自健康男性的精子样本分散于人工输卵管液（HTF）中，稀释到2×10⁷/mL后显微制样；利用数字全息显微镜记录包含精子三维位置信息的全息图；平均每组全息图得到背景图；对全息图做背景扣除后，利用定位算法获取精子的实时三维坐标；利用轨迹连接算法将精子的三维坐标连接成三维轨迹；基于三维轨迹，发展算法获取精子的三维运动模式、密度的空间分布、三维速度的空间分布和三维朝向。结果　利用数字全息显微镜，获取了包括精子的三维运动模式及反映精子与表面相互作用的密度分布、三维速度的空间分布和三维朝向在内的多项与精子受精能力相关的信息。结论　数字全息显微镜为分析和评估精子的受精能力提供了更为全面的数据。

Abstract

Objective　To apply digital holographic microscopy (DHM) as a tool for monitoring three-dimensional (3D) dynamics of human spermatozoa.　Methods　Sperm samples collected from healthy male participants were diluted to 2×107/mL by artificial human tubal fluid (HTF). These suspensions were injected for further observations. 3D dynamics of human spermatozoa were recorded by DHM with holograms. Background images were obtained by averaging holograms in each subgroup. Background removal was performed and the back-free hologram was applied for 3D localization of the sperm according to Rayleigh-Sommerfeld back propagation algorithm. Instantaneous 3D coordinates of human spermatozoa were linked to form trajectories. Subsequently, 3D motion patterns, density distribution, spatial distribution of 3D velocity and 3D orientation of human spermatozoa were determined.　Results　3D motility parameters, 3D motion patterns as well as information reflecting sperm-surface interactions including density distribution, spatial distribution of 3D velocity and 3D orientation of human spermatozoa were available by utilizing DHM. Conclusions　DHM can provide a comprehensive assessment of sperm fertilizing capacity.

导出引用

孙丽, 黄桂, 龚湘君, 欧建平. 基于数字全息显微镜的人类精子的三维动态行为监测技术[J]. 中国临床解剖学杂志. 2020, 38(4): 476-480 https://doi.org/10.13418/j.issn.1001-165x.2020.04.021

SUN Li, HUANG Gui, GONG Xiang-jun , OU Jian-ping. Digital holographic microscopy (DHM) and its application in monitoring three-dimensional dynamics of human spermatozoa[J]. Chinese Journal of Clinical Anatomy. 2020, 38(4): 476-480 https://doi.org/10.13418/j.issn.1001-165x.2020.04.021

中图分类号： R321.1

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