改良法制作兔右冠状动脉缺血再灌注损伤模型

doi:10.13418/j.issn.1001-165x.2020.03.022

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (3) : 351-354. DOI: 10.13418/j.issn.1001-165x.2020.03.022

技术方法

改良法制作兔右冠状动脉缺血再灌注损伤模型

马昌榕¹，　刘燕²，　蒋桔泉²

作者信息 +

Modified rabbit model of right coronary artery ischemia-reperfusion injury

MA Chang-rong¹, LIU Yan², JIANG Ju-quan²

Author information +

文章历史 +

摘要

目的　探讨兔右冠状动脉穿线结扎后放松建立兔右冠状动脉缺血再灌注损伤模型的可行性。方法　新西兰大白兔20只，雌雄不限，体重（2.5±0.3）kg，分为两组，缺血再灌注组14只，假手术组6只。假手术组右冠状动脉只穿线不结扎，另一组通过预置缝线结扎30 min后松开线结构建兔右冠状动脉缺血再灌注损伤模型。记录心电图情况、结扎前后肌酸激酶同功酶变化，术后软管插入离体心右冠状动脉验证结扎效果。结果　ST段明显抬高、软管插入右冠状动脉不易拉出并肌酸激酶同功酶升高8例，制模成功率57%（8/14）。假手术组上述指标无明显变化。结论　本实验改良制作右冠状动脉缺血再灌注模型，造模成功。

Abstract

Objective　To explore the feasibility of a simple rabbit model of right coronary artery ischemia-reperfusion injury by threading ligation.　Methods　20 New Zealand white rabbits, male or female, weighing (2.5±0.3) kg, were divided into two groups: an ischemia-reperfusion group (14 rabbits) and a sham operation group (6 rabbits). Threading without ligation were treated in the sham operation group, a model of rabbit right coronary artery ischemia-reperfusion injury was constructed after loosening the thread of 30 min preset suture ligation. The electrocardiogram was recorded, the changes of creatine kinase isoenzyme (CK-MB) before and after ligation, and the condition of postoperative hose insertion into the right crest were compared.　Results　The ST segment significantly elevated, the hose inserted into the right coronary artery was difficult to drag out and the creatine kinase isoenzyme increased in 8 cases. The success rate of the model was 57% (8/14). There were no significant changes among the above indicators in the sham operation group. Conclusions　This study explores the model of right coronary artery ischemia-reperfusion-reperfusion injury, and the modeling is basically successful.

导出引用

马昌榕, 刘燕, 蒋桔泉. 改良法制作兔右冠状动脉缺血再灌注损伤模型[J]. 中国临床解剖学杂志. 2020, 38(3): 351-354 https://doi.org/10.13418/j.issn.1001-165x.2020.03.022

MA Chang-rong, LIU Yan, JIANG Ju-quan. Modified rabbit model of right coronary artery ischemia-reperfusion injury[J]. Chinese Journal of Clinical Anatomy. 2020, 38(3): 351-354 https://doi.org/10.13418/j.issn.1001-165x.2020.03.022

中图分类号： R322.33

参考文献

[1] 陈伟伟, 杨跃进, 郑哲, 等.《中国心血管病报告2015》概要[J]. 中国循环杂志, 2016, 31(6): 521-528.
[2] 蒋桔泉. 急性心肌梗死后自主神经活性应分时段和区域进行研究[J]. 中国心脏起搏与心电生理杂志, 2015, 29(1): 1-2.
[3] 薛枫, 杨向军, 李勋, 等.兔在体心脏缺血再灌注模型的制作方法的改进[J]. 中国比较医学杂志, 2010, 20(2): 51-53, 64, 87.
[4] 刘志强, 程彦斌. 改良法创建兔心房室结缺血再灌注损伤模型[J]. 中国心血管杂志, 2005, 10(1): 43-45.
[5] 朱清於. 兔的冠状动脉解剖学[J]. 心肺血管学报, 1987, 6(3): 36-40.
[6] 周俐红, 王琦, 申彪, 等. 兔心脏冠状动脉的大体解剖[J]. 新乡医学院学报, 2003, 20(2): 96-97.
[7] 王庆志, 张黎声, 原林. 窦房结和房室结缺血再灌注动物模型的建立与确认[J]. 中国临床解剖学杂志, 2004, 22(5): 552-554.
[8] 王友, 王立赞, 王保生, 等. 利多卡因对缺血再灌注家兔心肌NF-kＢ和血浆ICAM-1 IL-8的影响[J]. 济宁医学院学报, 2012, 35(6): 399-401.
[9] 王静. 不同部位急性心肌梗死患者的心律失常及心率变异性分析[D]. 兰州：兰州大学, 2017.
[10]杨柏松, 陈用贵, 贾丹, 等. 单纯急性下壁心肌梗死和急性下壁合并右室心肌梗死的临床对比分析[J]. 临床军医杂志, 2014, 42(4): 373-375.
[11]Lu Z, Scherlag BJ, Lin J, et al. Autonomic mechanism for initiation of rapid firing from atria and pulmonary veins: evidence by ablation of ganglionated plexi[J]. Cardiovasc Res, 2009, 84(2): 245-252.
[12] 宋治远, 李永华, 姚青, 等. 兔右冠状动脉急性闭塞与再灌注时心律失常的发生与演变[J]. 中国心脏起搏与心电生理杂志, 2003, 17(1): 51-54.
[13] 张莉娟, 陈伯銮. 关注Bezold-Jarisch反射[J]. 国外医学: 麻醉学与复苏分册, 2004, 25(3): 181-183.