敲低剪接因子Prp17对小鼠肝组织形态学和肝功能的影响

doi:10.13418/j.issn.1001-165x.2020.02.018

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (2): 194-197.doi: 10.13418/j.issn.1001-165x.2020.02.018

敲低剪接因子Prp17对小鼠肝组织形态学和肝功能的影响

马文杰¹，　黄子进²，　窦健萍¹，　马春媚¹，　刘爱军¹，　王家有¹

广州中医药大学基础医学院　1.人体解剖学系，　2. 2015级生物技术专业，广州 510006

出版日期:2020-03-25 发布日期:2020-04-01
通讯作者: 王家有，副教授，硕士研究生导师， E-mail：xmishu@gzucm.edu.cn
作者简介:马文杰（1993-），回族，河南人，在读硕士，研究方向：中西医结合防治酒精性肝病，E-mail：1224644454@qq.com
基金资助:
广州市科技计划项目（No.201804010311）

Effect of knockdown splicing factor Prp17 on liver morphology and liver function in mice

MA Wen-jie¹，HUANG Zi-jin², DOU Jian-ping¹, MA Chun-mei¹, LIU Ai-jun¹，WANG Jia-you¹

1. Department of Human Anatomy, School of Fundamental Medical Science, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, Guangdong Province, China; 2. Department of 2015 Grade Biotechnology Major, School of Fundamental Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China

Online:2020-03-25 Published:2020-04-01

摘要/Abstract

摘要： 目的　探讨敲低剪接因子Prp17对小鼠肝组织形态学和肝功能的影响。方法　构建敲低剪接因子Prp17腺病毒（Ad-shPrp17）。随机将C57BL/6小鼠分为Control组和Ad-shPrp17组，每组8只。Ad-shPrp17组通过内眦静脉注射Ad-shPrp17。10 d后采集小鼠血清和收集肝组织。用苏木精-伊红染色法观察肝组织形态学变化；酶法测定血清谷草转氨酶（AST）和谷丙转氨酶（ALT）活性；荧光定量聚合酶链式反应分析基因表达情况。结果　与Control组比较，Ad-shPrp17组小鼠：（1）肝的质量和肝指数显著增加（P<0.01）；（2）肝细胞排列散乱，细胞肿胀，胞质疏松化，肝细胞由多角形变成圆形，胞质透明，呈气球样变，部分细胞发生坏死；（3）血清AST和ALT的酶活力显著升高（P<0.01）；（4）肝组织TNF-α、IL-1β表达升高（P<0.05）。结论　敲低剪接因子Prp17可造成小鼠肝组织形态发生病理改变和肝功能损伤，其机制可能与诱导肝炎症反应有关。这为肝疾病的防治提供新的治疗靶点和理论依据。

关键词: , 剪接因子；　Prp17；　肝组织形态学；　肝功能；　炎症

Abstract: Objective To investigate the effects of knockdown splicing factor Prp17 on liver morphology and liver function in mice. Methods The knockdown splicing factor Prp17 adenovirus (Ad-shPrp17) was constructed. C57BL/6 mice were randomly divided into a control group and an Ad-shPrp17 group with 8 mice in each group. The Ad-shPrp17 group was injected with Ad-shPrp17 through medial canthus vein. After 10 days, mouse serum and liver tissue were collected. Pathological changes of liver were observed by hematoxylin-eosin staining. The activities of serum aspartate transaminase (AST) and alanine transaminase (ALT) were determined by enzyme method. Gene expression was analyzed by fluorescence quantitative polymerase chain reaction. Results Compared with the control group, the Ad-shPrp17 group had the following changes: (1) Liver index and the quality of liver significantly increased (P<0.01); (2) with scattered and swollen hepatocytes, loose cytoplasm, the liver cells changed from polygonal to round. The cytoplasm was transparent and ballooning changed, and some cells were necrotic; (3) The enzyme activities of serum ALT and AST were significantly increased (P<0.01); (4) The expression of liver tissue TNF-α and IL1-β increased (P<0.05). Conclusions Knockdown of splicing factor Prp17 could cause pathological changes and function damage in the liver tissue of mice, and its mechanism may be related to the induction of hepatic inflammatory response. This study provides a new therapeutic target and theoretical basis for the prevention and treatment of liver diseases.

Key words: Splicing factor;　 Prp17, 　Liver morphology, 　Liver function, 　Inflammation

中图分类号:

马文杰, 黄子进, 窦健萍, 马春媚, 刘爱军, 王家有. 敲低剪接因子Prp17对小鼠肝组织形态学和肝功能的影响[J]. 中国临床解剖学杂志, 2020, 38(2): 194-197.

MA Wen-jie, HUANG Zi-jin, DOU Jian-ping, MA Chun-mei, LIU Ai-jun, WANG Jia-you. Effect of knockdown splicing factor Prp17 on liver morphology and liver function in mice[J]. Chinese Journal of Clinical Anatomy, 2020, 38(2): 194-197.

参考文献 10

[1]	Pan Q, Shai O, Lee LJ,et al. Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing[J]. Nat Genet, 2008, 40(12): 1413-1415.
[2]	Wang J, Kainrad N, Shen T, et al. Hepatic knockdown of splicing regulator slu7 ameliorates inflammation and attenuates liver injury in ethanol-fed mice[J]. Am J Pathol, 2018, 188(8): 1807-1819.
[3]	Kaplan Y, Kupiec M. A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition[J]. Curr Genet, 2007, 51(2): 123-140.
[4]	Kong XX, Lv YR, Shao LP, et al. HBx-induced MiR-1269b in NF-κB dependent manner upregulates cell division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells[J]. J Transl Med, 2016, 14(1): 189-202.
[5]	Cheng Y, Luo C, Wu W, et al. Liver-Specific Deletion of SRSF2 caused acute liver failure and early death in mice[J].Mol Cell Biol, 2016, 36(11): 1628-1638.
[6]	Sen S, Langiewicz M, Jumaa H, et al. Deletion of serine/arginine-rich splicing factor 3 in hepatocytes predisposes to hepatocellular carcinoma in mice[J]. Hepatology, 2015, 61(1): 171-183.
[7]	Elizalde M, Urtasun R, Azkona M, et al. Splicing regulator SLU7 is essential for maintaining liver homeostasis[J]. J Clin Invest, 2014, 124(7): 2909-2920.
[8]	王超玄, 侯宇, 陈哲, 等. 肝脏特异性DEK基因敲除小鼠模型的制备及鉴定[J]. 中国临床解剖学杂志, 2018, 36(4): 397-407.
[9]	Su LX, Pan P, Wang XT, et al.Vimentin modulates apoptosis and inflammatory cytokine release by a human monocytic cell line (THP-1) in response to lipopolysaccharides in vitro[J]. Chin Med J (Engl), 2019, 132(11): 1336-1343.
[10]	Tulotta C, Lefley DV, Freeman K, et al. Endogenous production of IL1B by breast cancer cells drives metastasis and colonization of the bone microenvironment[J]. Clin Cancer Res, 2019, 25(9): 2769-2782.

[1]	江剑宏, 段仁鹏, 李晓锋. 国人胆道三维重建解剖变异应用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 1-4.
[2]	郭红志, 王瑜, 张加琪, 梁海彬, 马梓玮, 冯伟, 吴忧, 司子燚. 血管铸型结合CT三维模型评估先兆子痫胎盘血管结构[J]. 中国临床解剖学杂志, 2024, 42(1): 5-10.
[3]	李佳伟, 张静, 李灿然, 兰文杰, 籍庆余, 郭志勇, 张云凤, 刘启, 陈清威, 李筱贺. 蒙古族人群股骨近端解剖参数X线测量[J]. 中国临床解剖学杂志, 2024, 42(1): 11-16.
[4]	李琨, 张燕, 郭冉, 郝咪咪, 吴轩宇, 徐艺芳, 王超群, 马文童, 张灵淇, 杨宏宇, 李志军, 张少杰, 王星. 儿童及青少年枕颈角与后枕颈角数字化测量及其临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 17-20.
[5]	李文, 杨思艺, 黄蕾, 卿霁雯, 蒋松涛, 张磊. 基于MRI探讨Lisfranc韧带的形态学特点及临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 21-25.
[6]	李凡凡, 徐阳, 王晓旭. 紫草素调节Nrf2/HO-1信号通路对实验性大鼠肉芽肿性小叶性乳腺炎的治疗作用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 26-32.
[7]	王兴航, 丁佳媛, 李放, 包翠芬, 阎丽菁. 人参皂苷Rg1通过抑制NLRP3炎症小体途径减轻氧糖剥夺/复供后小胶质细胞炎症反应[J]. 中国临床解剖学杂志, 2024, 42(1): 33-41.
[8]	程莹莹, 武建军, 蔡海燕, 焦旭文, 马江波, 丁银秀. 体外炎性诱导星形胶质细胞激活及功能分析[J]. 中国临床解剖学杂志, 2024, 42(1): 42-46.
[9]	祁志, 杨力, 贾秋叶, 陈浩伦, 段兆达, 吴春云. 依达拉奉经MAPKs通路对脂多糖激活的小胶质细胞Sirt3表达调控[J]. 中国临床解剖学杂志, 2024, 42(1): 47-53.
[10]	张晶晶, 王洪新. 黄芩苷通过PDGF/P38 MAPK信号通路对肺动脉高压大鼠的保护作用[J]. 中国临床解剖学杂志, 2024, 42(1): 54-58.
[11]	乐钦, 陈荣丽, 熊婧熙, 王婷怡, 蔡志恒, 易秋实, 曾心怡. 白芨多糖对大鼠跨区皮瓣choke区的影响[J]. 中国临床解剖学杂志, 2024, 42(1): 59-64.
[12]	李笑予, 张磊, 付磊, 李东波, 汪国友. 三步接骨法治疗Sanders Ⅱ型跟骨骨折的有限元研究[J]. 中国临床解剖学杂志, 2024, 42(1): 65-70.
[13]	吴研飞, 马剑雄, 卢斌, 王颖, 柏豪豪, 靳洪震, 马信龙. CT后处理技术重建CT值与终板抗压强度的相关性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 71-76.
[14]	庄万强, 唐毅, 骆勇刚, 张辉. 关节镜联合胫骨高位截骨术对髌骨位置及髌股关节功能影响的早中期回顾性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 77-82.
[15]	罗鹰, 窦伟誉, 吴小杭, 陈璟昆, 彭昌贵, 潘剑英 . 微创海马钢板内固定治疗跟骨骨折并发症的影响因素[J]. 中国临床解剖学杂志, 2024, 42(1): 83-88.

敲低剪接因子Prp17对小鼠肝组织形态学和肝功能的影响

Effect of knockdown splicing factor Prp17 on liver morphology and liver function in mice

可视化

摘要/Abstract

引用本文

使用本文

参考文献 10

相关文章 15

Metrics

本文评价

推荐阅读 0