目的 研究莫诺苷对血管性痴呆模型大鼠的作用及其机制。 方法 大鼠随机分为假手术组(Sham组)、模型组(VD组)、VD+Mor(25、50和100 mg)组，灌胃给药1次/d，连续28 d。Morris水迷宫测试空间学习记忆能力；HE染色观察脑组织病理学改变；TUNEL法检测海马细胞凋亡；ELISA测定血清SOD、MDA和LDH水平；Western Blot法检测海马Bax/Bcl-2，cyto胞质水平，caspase-9，caspase-3和p53蛋白表达水平。 结果 莫诺苷改善血管痴呆大鼠学习记忆能力；抑制脑组织病理学改变；降低细胞凋亡；提高血清SOD，降低MDA、LDH含量；并抑制Bax/Bcl-2，cyto胞质水平，caspase-9、caspase-3及p53蛋白表达。 结论 莫诺苷改善血管性痴呆大鼠的记忆能力和病理改变，降低脑组织细胞凋亡率和自由基水平，抑制线粒体凋亡通路蛋白表达，对血管性痴呆大鼠起到治疗作用。
Abstract
Objective To study the effect and mechanism of morroniside(Mor) on the vascular dementia(VD) model rats. Methods VD model rats were randomly divided into sham operation group (Sham group), model group (VD group), VD+Mor (25, 50 and 100 mg) groups. Rats were intragastric administration Mor once a day for 28 days. The spatial learning and memory ability were measured by Morris water maze. Histopathological changes of brain issue, apoptosis rate of hippocampus were detected by HE and TUNEL staining respectively. The serum levels of SOD, MDA and LDH were measured by ELISA, the protein expression of Bax/Bcl-2, cytochrome C, caspase-9, caspase-3 and p53 in hippocampus were measured by Western Blot. Results Morroniside improved the learning and memory ability of VD rats in a dose-dependent manner, inhibited the histopathological changes of brain tissue; decreased the apoptosis rate, increased the serum level of SOD, reduced the content of MDA and LDH, and inhibited the protein expression of Bax/Bcl-2 ratio, cytochrome c, caspase-9, caspase-3 and p53. Conclusions Morroniside improves the memory ability and pathological changes of VD rats, reduces the apoptotic rate and free radical level of brain tissue, and inhibits the protein expression of mitochondrial apoptotic pathway, thus playing a therapeutic role in VD rats.
关键词
血管性痴呆 /
丝氨酸 /
线粒体凋亡途径
Key words
Vascular dementia /
Morroniside /
Mitochondrial apoptotic pathway
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基金
北京市科委科技计划重大项目(D171100003117005)；
京ICP备08002354号-3
