灵长类动物子宫内膜异位症病证结合模型构建初探

doi:10.13418/j.issn.1001-165x.2019.05.013

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (5) : 547-552. DOI: 10.13418/j.issn.1001-165x.2019.05.013

实验研究

灵长类动物子宫内膜异位症病证结合模型构建初探

李舒婷¹，　宗利丽²，　何伟峰¹，　郜洁²，　饶军华³，　李比海³，　刘贻颜³，　刘玉洁¹，
冯倩¹，　王庭伟⁴，　刘凌子²，　伍庭凤⁵，　汪碧云¹，　聂伟哲¹

作者信息 +

Preliminary study on the construction of primate model combined with disease of endometriosis and syndyome

LI Shu-ting¹，ZONG Li-li²，HE Wei-feng¹，GAO Jie²，RAO Jun-hua³，LI Bi-hai³，LIU Yi-yan³，LIU Yu-jie¹，FENG Qian¹，WANG Ting-wei ⁴， LIU Ling-zi²，WU Ting-feng⁵，WANG Bi-yun¹，NIE Wei-zhe¹

Author information +

文章历史 +

摘要

目的　探索食蟹猴子宫内膜异位症（简称内异症）病证结合模型构建的方法。方法　（1）6只雌性食蟹猴随机分为实验组5只、对照组1只，实验组开腹行宫颈环扎术，对照组仅开腹。（2）于术前、术中及术后第1、2、3个月运用PSI测量猴舌血液灌注量。（3）术后3个月腹腔镜探查，因均未见内异症病灶，改进建模方法：将猴分为实验组4只、对照组2只，实验组月经期收集子宫内膜组织盆腔内注射，连续使用3个月免疫抑制剂，对照组不作处理。结果　（1）宫颈环扎后3个月后腹腔镜探查，两组均未见内异症病灶。（2）猴子术后2月舌部血液灌注量明显高于对照组，差异有统计学意义（P<0.05）。（3）每只猴的舌面及舌底血液灌注量变化趋势一致。（4）改进建模方案后3个月腹腔镜探查示，实验组重度盆腔粘连、子宫表面血管增生并充血明显，一只猴可见内异症病灶，对照组则无。结论　收集子宫内膜盆腔内注射、加用免疫抑制剂可以构建食蟹猴内异症模型。运用PSI测量猴舌部血液灌注量的方法稳定可行，有望成为疾病病证结合模型舌象的量化评价方法。

Abstract

Objective　To explore the method of constructing the primate model combined with disease of endometriosis and syndrome.　Methods　(1) Six female cynomolgus monkeys were randomly divided into a modeling group (n=5) and a control group (n=1). The modeling group was treated with transabdomal cervical cerclage while the control group was only observed through an open surgery. (2) The blood perfusion of the tongue was measured by PeriCam PSI perfusion speckle imager before the first modeling operation, at the modeling month and after the first, second and third months of modeling. (3) Laparoscopic exploration was performed three months after the operation. Since no endometriosis lesions were found in the pelvis of the monkey, we changed the modeling method: the monkeys were re-assigned into the modeling group (n=4) and the control group(n=2). In the modeling group, the endometrial tissue fragments were collected during the menstrual period and intraperitoneal injection, and immunosuppressive agents were administered for three months.　Results:　(1) In the laparoscopy after three months of cervical cerclage, no endometriosis lesion was seen in the two groups. (2) The blood perfusion in the monkeys was significantly higher in the modeling group than that in the control group at 2 months after the operation (P<0.05).(3) The trend of blood perfusion in the surface and base of the tongue of each monkey was consistent.(4) Three months after the intraperitoneal injection with the immunosuppressive agents, the modeling group had severe pelvic adhesions, vascular hyperplasia and hyperemia on the serosa of the uterine under the laparoscopy. The endometriosis lesions were found in one of the modeling monkeys. Conclusion The endometrial pelvic injection with the administration of the immunosuppressive agents is a feasible approach for constructing primate endometriosis models. The method of measuring the blood perfusion of the tongue of the cynomolgus monkey using PSI is easy and stable, and it may become a quantitative evaluation method for the tongue image of the disease syndrome combination model.

导出引用

李舒婷, 宗利丽, 何伟峰, 郜洁, 饶军华, 李比海, 刘贻颜, 刘玉洁, 冯倩, 王庭伟, 刘凌子, 伍庭凤, 汪碧云, 聂伟哲. 灵长类动物子宫内膜异位症病证结合模型构建初探[J]. 中国临床解剖学杂志. 2019, 37(5): 547-552 https://doi.org/10.13418/j.issn.1001-165x.2019.05.013

LI Shu-ting, ZONG Li-li, HE Wei-feng, GAO Jie, RAO Jun-hua, LI Bi-hai, LIU Yi-yan, LIU Yu-jie, FENG Qian, WANG Ting-wei, LIU Ling-zi, WU Ting-feng, WANG Bi-yun, NIE Wei-zhe. Preliminary study on the construction of primate model combined with disease of endometriosis and syndyome[J]. Chinese Journal of Clinical Anatomy. 2019, 37(5): 547-552 https://doi.org/10.13418/j.issn.1001-165x.2019.05.013

中图分类号： R711.71　

参考文献

[1] Stratton P, Berkley K J. Chronic pelvic pain and endometriosis: translational evidence of the relationship and implications[J]. Hum Reprod Update, 2011,17(3):327-346.
[2] 李雷. 子宫内膜异位症卫生经济学的研究进展[J]. 现代妇产科进展, 2013,22(7):596-598.

[3] Tirado-González I, Barrientos G, Tariverdian N, et al. Endometriosis research: animal models for the study of a complex disease[J]. J Reprod Immunol, 2010, 86(2):141-147. 　

[4] 殷惠军, 黄烨. 病证结合动物模型的研究进展[J]. 中国中西医结合杂志, 2013,33(1):8-10.
[5] 邓铁涛. 中医诊断学[M]. 上海: 上海科学技术出版社, 2012.
[6] 宗利丽, 李亚里, 汪龙霞, 等. 子宫内膜异位症猕猴动物模型的建立[J]. 第一军医大学学报, 2003,23 (10):1006-1009.
[7] Briers D, Duncan D D, Hirst E, et al. Laser speckle contrast imaging: theoretical and practical limitations[J]. Journal of biomedical optics, 2013,18(6):66018.
[8] 邸丹, 周敏, 周会林, 等. 手持式舌象仪的研制[J]. 上海中医药杂志, 2016, 50(2):12-14.
[9] Daraï E, Ploteau S, Ballester M, et al. Endométriose : physiopathologie, facteurs génétiques et diagnostic clinique[J]. La Presse Médicale, 2017,46(12):1156-1165.
[10]Yamanaka A, Kimura F, Takebayashi A, et al. Primate Model Research for Endometriosis[J]. Tohoku J Exp Med, 2012, 226(2):95-99.
[11]张娜. 子宫内膜异位症的病因学研究进展[J]. 实用临床医学, 2016,17(01):104-107.
[12]俞超芹. 子宫内膜异位症免疫微环境形成机制及中药干预作用[J]. 中国中西医结合杂志, 2019,39(5):517-520.
[13]罗大中. 数字舌图舌色标准化方法研究[D]. 北京中医药大学, 2008.
[14]吴朦, 胡镜清. 舌下络脉诊法及其在血瘀辨证中的应用研究[J]. 环球中医药, 2015,8(6):646-649.
[15]Dubin A, Pozo M O, Ferrara G, et al. Systemic and microcirculatory responses to progressive hemorrhage[J]. Intensive Care Medicine, 2009,35(3):556-564.
[16]Kim T S, Hanak M, Trampont P C, et al. Stress-associated erythropoiesis initiation is regulated by type 1 conventional dendritic cells[J]. The Journal of clinical investigation, 2015,125(10):3965-3980.
[17]Alamo I G, Kannan K B, Loftus T J, et al. Severe trauma and chronic stress activates extramedullary erythropoiesis[J]. J Trauma Acute Care Surg, 2017,83(1):144-150.
[18]黄增, 徐杰丰, 陈国锋, 等. 舌下微循环变化在猪失血严重程度评估中的应用价值[J]. 中华危重症医学杂志(电子版), 2016,9(5):328-333.