葛根素对臂丛神经根性撕脱伤脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响

doi:10.13418/j.issn.1001-165x.2019.05.008

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (5): 517-522.doi: 10.13418/j.issn.1001-165x.2019.05.008

葛根素对臂丛神经根性撕脱伤脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响

王雨¹，　陈传奇²，　王文晟¹，　喻保军¹，　张义¹，　李丹¹，　陈龙菊¹

1.湖北民族大学医学部解剖学教研室，湖北恩施 445000； 2.湖北民族大学附属
恩施州土家族苗族自治州中心医院妇产科，湖北恩施 445000

收稿日期:2019-02-15 出版日期:2019-09-25 发布日期:2019-09-25
通讯作者: 陈龙菊，博士，教授，硕士生导师，Tel：0718-8437479，E-mail：1997029@hbmy.edu.cn
作者简介:王雨（1993-），男，在读硕士，研究方向：中医药治疗周围神经损伤，E-mail：201630160@hbmy.edu.cn
基金资助:
国家自然科学基金（81260192）；湖北民族大学博士科研项目（MY2018B026）

Effects of Puerarin on the expression of iNOS, CGRP, and PI3K/Akt signaling pathway in spinal cord anterior horn after brachial plexus root avulsion injury

WANG Yu¹, CHEN Chuan-qi², WANG Wen-sheng¹, YU Bao-jun¹, ZHANG Yi¹, LI Dan¹, CHEN Long-ju¹

1.Department of Anatomy, Health Science Center, Hubei Minzu University, Enshi 445000, Hubei Province, China; 2.Department of Obstetrics and Gynecology the Central Hospital of Tujia and Miao Autonomous Prefectur，Hubei Minzu University, Enshi 445000, Hubei Province, China

Received:2019-02-15 Online:2019-09-25 Published:2019-09-25

摘要/Abstract

摘要： 目的　探讨葛根素对臂丛神经根性撕脱伤（brachial plexus root avulsion injury，BPRAI）脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响。方法　将50只雄性SD大鼠随机分为正常组、模型组、葛根素低、中、高剂量治疗组，每组10只。模型组，葛根素低、中、高剂量治疗组进行BPRAI造模，撕脱大鼠右侧C5~7脊神经前根，后根剪断，术后3个治疗组予腹腔注射葛根素，剂量分别为50、100、200 mg·kg-1·d-1，正常组、模型组腹腔注射等体积生理盐水，持续4周。采用尼氏染色、免疫荧光化学、Western blot方法，观察损伤侧脊髓前角α运动神经元（alpha motorneurons，α-MNs）的存活率，iNOS、CGRP、PI3K/Akt通路相关蛋白的表达。结果　第4周时，低、中、高剂量的葛根素治疗可抑制α-MNs丢失（P<0.05或P<0.01）；中、高剂量的葛根素治疗可抑制iNOS表达（P<0.05）；高剂量的葛根素治疗可促进CGRP蛋白表达（P<0.05或P<0.01）；低、中、高剂量葛根素均可显著抑制p-Akt1/2/3表达（P<0.01）。结论　葛根素可改善BPRAI造模引起的α-MNs死亡，其机制可能与葛根素能抑制iNOS蛋白的表达、促进CGRP蛋白的表达有关，并且PI3K/Akt信号通路参与其调控。

关键词: 葛根素, 　臂丛神经损伤, 　α运动神经元, 　iNOS, 　CGRP, 　PI3K/Akt

Abstract: Objective　To investigate the effect of Puerarin on the expression of iNOS, CGRP and PI3K/Akt signaling pathway in spinal anterior horn following brachial plexus root avulsion injury (BPRAI). Methods　Fifty SD rats were randomly divided into a normal group, a model group, a low-dose group, a middle-dose group and a high-dose group with 10 rats in each group. BPRAI modeling was made in the model group, low-dose group, middle-dose group and high-dose group by avulsing the anterior roots and transecting posterior roots of right C5~7 segment of spinal nerve. Puerarin was injected intraperitoneally in the low-dose group, middle-dose group and high-dose group after the operation at doses of 50, 100 and 200 mg·kg-1·d-1 respectively. The normal group and model group were given intraperitoneal injection of saline of equal volume for 4 weeks. Nissl staining, immunofluorescence and Western blot were used to observe the survival rate, the expression of iNOS and CGRP of alpha motorneurons (α-MNs), and PI3K/Akt pathway related proteins in the spinal cord on the injured side. Results At the 4th week, low, middle and high doses of Puerarin could inhibit the loss of α-MNs (P<0.05 or P<0.01); middle and high doses of Puerarin could inhibit the expression of iNOS (P<0.05); high doses of Puerarin could significantly promote the expression of CGRP protein (P<0.05 or P<0.01); low, middle and high doses of Puerarin could significantly inhibit the expression of p-Akt1/2/3 (P<0.01). Conclusions Puerarin can ameliorate the death of alpha-MNs induced by BPRAI. The protective effect of Puerarin may be related to its inhibition of iNOS expression and promotion of CGRP expression, and PI3K/Akt signaling pathway is involved in its regulation.

Key words: Puerarin; , Brachial plexus root injury; , Alpha motorneurons; , iNOS; , CGRP; , PI3K/Akt

中图分类号:

R745.41

王雨, 陈传奇, 王文晟, 喻保军, 张义, 李丹, 陈龙菊. 葛根素对臂丛神经根性撕脱伤脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响[J]. 中国临床解剖学杂志, 2019, 37(5): 517-522.

WANG Yu, CHEN Chuan-qi, WANG Wen-sheng, YU Bao-jun, ZHANG Yi, LI Dan, CHEN Long-ju. Effects of Puerarin on the expression of iNOS, CGRP, and PI3K/Akt signaling pathway in spinal cord anterior horn after brachial plexus root avulsion injury[J]. Chinese Journal of Clinical Anatomy, 2019, 37(5): 517-522.

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葛根素对臂丛神经根性撕脱伤脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响

Effects of Puerarin on the expression of iNOS, CGRP, and PI3K/Akt signaling pathway in spinal cord anterior horn after brachial plexus root avulsion injury

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