党参多糖介导Nrf2通路对缺氧缺血性脑损伤的抗氧化和神经保护作用

doi:10.13418/j.issn.1001-165x.2019.04.009

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (4): 403-408.doi: 10.13418/j.issn.1001-165x.2019.04.009

党参多糖介导Nrf2通路对缺氧缺血性脑损伤的抗氧化和神经保护作用

马竞¹，　何文龙¹，　高重阳¹，　余瑞云¹，　薛鹏¹，　牛永超²

新乡市中心医院 1. 神经内科， 2. CT室，河南新乡 453000

收稿日期:2019-01-18 出版日期:2019-07-25 发布日期:2019-08-01
作者简介:马竞（1982-）, 男，本科，主治医师，研究方向：神经内科，E-mail：yidi48143@sina.com
基金资助:
河南省医学科技公关计划项目（201602168）

Antioxidant and neuroprotective effects of Codonopsis pilosula polysaccharides on hypoxic-ischemic brain injury induced by Nrf2 pathway

MA Jing¹, HE Wen-long¹, GAO Chong-yang¹, YU Rui-yun¹, XUE Peng¹, NIU Yong-chao²

Xinxiang Central Hospital: 1.Department of Neurology, 2.CT Room, Xinxiang 453000, Henan Province, China

Received:2019-01-18 Online:2019-07-25 Published:2019-08-01

摘要/Abstract

摘要： 目的　研究党参多糖对缺氧缺血性脑损伤的抗氧化和神经保护作用及其机制。方法　采用Rice法建立HIBI模型。假手术组和模型组灌胃给予生理盐水，模型加药组灌胃给予党参多糖。分别进行神经功能学评分，观察脑积水量，脑组织病理学改变，神经元细胞凋亡情况，脑组织脂质过氧化物水平，抗氧化和神经保护相关蛋白表达水平。结果　党参多糖能显著改善模型大鼠神经功能、脑水肿（P<0.01）和病理改变，降低细胞凋亡率（P<0.01）和Bax表达（P<0.01），降低LDH和MDA含量（P<0.01）；同时，上调Bcl-2表达（P<0.01）和SOD活性（P<0.01），增加bFGF、BDNF、PSD95、SYP、Nrf2和HO-1表达（P<0.01）。结论　党参多糖对缺氧缺血性脑损伤具有抗氧化和神经保护作用，可能与介导Nrf2信号通路相关。

关键词: 缺氧缺血性脑损伤, 　党参多糖, 　核转录相关因子2, 　醌氧化还原酶1

Abstract: Objective　To study the antioxidant and neuroprotective effects of Codonopsis pilosula polysaccharides (CPP) and its possible mechanism.　Methods　Rice method was used to establish HIBI rat model. Normal saline was given to the sham group and the HIBI group, and corresponding doses of CPP solution were given to the drug groups. The neurological function, cerebral water content of rats, histopathologic changes, apoptosis in the rat hippocampus, the level of lipid peroxide, the expression of antioxidant and neuroprotective proteins were evaluated.　Results　Compared with the model group, the neurological function, cerebral water content and the pathological injury of brain tissue were significantly reduced (P<0.01). The apoptosis rate, expression of Bax, content of LDH and MDA also decreased significantly (P<0.01). Expression of Bcl-2 and activity of SOD were up-regulated (P<0.01). In addition, the expressions of bFGF, BDNF, PSD95, SYP, Nrf2 and HO-1 were significantly up-regulated (P<0.01). Conclusion　CPP has antioxidant and neuroprotective effects on hypoxic-ischemic brain injury, and its function may be related to mediating Nrf2 signal pathway.

Key words: Hypoxic ischemic brain injury, 　Codonopsis pilosula polysaccharides, 　Nuclear transcription related factor 2, 　Oxidoreductase 1

中图分类号:

R743

马竞, 何文龙, 高重阳, 余瑞云, 薛鹏, 牛永超. 党参多糖介导Nrf2通路对缺氧缺血性脑损伤的抗氧化和神经保护作用[J]. 中国临床解剖学杂志, 2019, 37(4): 403-408.

MA Jing, HE Wen-long, GAO Chong-yang, YU Rui-yun, XUE Peng, NIU Yong-chao. Antioxidant and neuroprotective effects of Codonopsis pilosula polysaccharides on hypoxic-ischemic brain injury induced by Nrf2 pathway[J]. Chinese Journal of Clinical Anatomy, 2019, 37(4): 403-408.

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党参多糖介导Nrf2通路对缺氧缺血性脑损伤的抗氧化和神经保护作用

Antioxidant and neuroprotective effects of Codonopsis pilosula polysaccharides on hypoxic-ischemic brain injury induced by Nrf2 pathway

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