丹参酮IIA磺酸钠注射液对心肌缺血再灌注大鼠心功能和免疫反应的调节作用

doi:10.13418/j.issn.1001-165x.2019.04.008

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (4): 397-402.doi: 10.13418/j.issn.1001-165x.2019.04.008

丹参酮IIA磺酸钠注射液对心肌缺血再灌注大鼠心功能和免疫反应的调节作用

李嫚，　王振军，　刘洁云，　秦雷

开封市中心医院心内科，河南开封 475000

收稿日期:2019-01-21 出版日期:2019-07-25 发布日期:2019-08-01
作者简介:李嫚（1977-），女，硕士，副主任医师，研究方向：冠脉方面疾病的研究与治疗，E-mail：13592120897@163.com
基金资助:
河南省医学科技攻关计划项目（201504085）

Regulatory effect of sodium tanshinone IIA sulfonate on cardiac function and immune response in rats with myocardial ischemia-reperfusion injury

LI Man, WANG Zhen-jun, LIU Jie-yun, QIN Lei

Department of Cardiology, Kaifeng City Central Hospital, Kaifeng 475000, Henan Province, China

Received:2019-01-21 Online:2019-07-25 Published:2019-08-01

摘要/Abstract

摘要： 目的　本文研究丹参酮IIA磺酸钠注射液（Sodium tanshinone IIA sulfonate，TIIA）对心肌缺血再灌注（ischemia-reperfusion，I/R）大鼠心功能和免疫反应的调节作用及其机制。方法　采用冠状动脉结扎法建立心肌缺血再灌注大鼠模型；随机分成5组：Ctrl组、I/R组、I/R+TIIA（0.5 mL）组、I/R+TIIA（1 mL）组和I/R+TIIA（2 mL）组；测定平均动脉压（mean ventricular systolic pressure，MAP）、左室收缩压（left ventricular systolic pressure，LVSP）和心率（heart rate，HR）；采集血清Elisa检测心损标记物表达水平和氧化应激指标含量；HE染色和TUNEL染色观察心肌损伤情况；流式分选外周血CD4+iNOS+，CD4+IL-10+凋亡变化；蛋白印迹法检测凋亡相关蛋白和P65、IL-8及TNF-α表达情况。结果　丹参酮ⅡA磺酸钠注射液能上调MAP、HR和LVSP水平以改善I/R模型大鼠心功能(P＜0.01)；降低血清中CK-MB、cTnΙ和Mb的表达上调；减少MDA、LDH表达，增加SOD水平；改善心肌组织的病理改变，抑制心肌细胞凋亡，上调Bcl-2、下调Bax蛋白表达(P＜0.01)；减少iNOS+和增加IL-10+细胞凋亡而改善Th1/Th2平衡(P＜0.01)；抑制P-P65、IL-8和TNF-α的表达下调(P＜0.01)。结论丹参酮IIA磺酸钠注射液对心肌缺血再灌注损伤具有保护作用，其机制与改善心功能，调节免疫反应有关。

关键词: 丹参酮IIA磺酸钠注射液, 　平均动脉压, 　左室收缩压, 　Th1/Th2

Abstract: Objective To investigate the effects of sodium tanshinone IIA sulfonate on cardiac function and immune response in rats with myocardial ischemia-reperfusion (I/R) injury and its mechanism. Methods The model of myocardial ischemia-reperfusion was established by coronary artery ligation. The rats were randomly divided into five groups: Ctrl group, I/R group, I/R+TIIA (0.5 mL), I/R+TIIA (1 mL) group and I/R+TIIA (2 mL) group. The mean ventricular systolic pressure (MAP), left ventricular systolic pressure (LV) and heart rate (HR) were measured. The expression of heart damage markers and the content of oxidative stress indicators were detected by Elisa test. HE staining and TUNEL staining were used to evaluate myocardial injury. The changes of CD4+iNOS+ and CD4+IL-10+ apoptosis in peripheral blood were evaluated by flow cytometry. Western blotting was used to detect the expression of apoptosis-related proteins and P65, IL-8 and TNF-α. Results Sodium tanshinone II A sulfonate injection could up-regulate the levels of MAP, HR and LVSP to improve the cardiac function of I/R model rats (P<0.01), reduce the expression of CK-MB, cTnΙ and MB in serum, and reduce the expression of MDA and LDH while increase SOD, improve the pathological changes of myocardial tissue, inhibit myocardial apoptosis, and up-regulate Bcl-2 and down-regulate the Bax (P<0.01), decrease apoptosis of iNOS+ and increase IL-10+ in order to improve Th1/Th2 balance (P<0.01), inhibit the down-regulation of P-P65, IL-8 and TNF-α expression induced by I/R injury (P<0.01). Conclusions Sodium tanshinone IIA sulfonate injection has protective effect on myocardial ischemia-reperfusion injury which is related to improving cardiac function and regulating immune response.

Key words: Sodium tanshinone IIA sulfonate, 　MAP, 　LVSP, 　Th1/Th2

中图分类号:

R541

李嫚, 王振军, 刘洁云, 秦雷. 丹参酮IIA磺酸钠注射液对心肌缺血再灌注大鼠心功能和免疫反应的调节作用[J]. 中国临床解剖学杂志, 2019, 37(4): 397-402.

LI Man, WANG Zhen-jun, LIU Jie-yun, QIN Lei. Regulatory effect of sodium tanshinone IIA sulfonate on cardiac function and immune response in rats with myocardial ischemia-reperfusion injury[J]. Chinese Journal of Clinical Anatomy, 2019, 37(4): 397-402.

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丹参酮IIA磺酸钠注射液对心肌缺血再灌注大鼠心功能和免疫反应的调节作用

Regulatory effect of sodium tanshinone IIA sulfonate on cardiac function and immune response in rats with myocardial ischemia-reperfusion injury

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