miR-143-3p 靶向MAPK1对人结肠癌细胞增殖，凋亡和侵袭的调节作用#br#

doi:10.13418/j.issn.1001-165x.2019.03.011

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (3) : 292-298. DOI: 10.13418/j.issn.1001-165x.2019.03.011

张志谦¹，　张广星²，　彭小东²

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The regulatory effects of miR-143-3p on proliferation, apoptosis and invasion on human colon cancer cell via targeting MAPK1

ZHANG Zhi-qian¹, ZHANG Guang-xin², PENG Xiao-dong²

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摘要

目的　研究miR-143-3p靶向MAPK1与人结肠癌细胞增殖、凋亡和侵袭的关系。方法　qRT-PCR检测miR-143-3p mimic转染效果和MAPK1的mRNA表达水平；双荧光素酶报告实验分析miR-143-3p与MAPK1的靶向关系；蛋白质印迹检测MAPK1的蛋白表达水平，CCK-8检测细胞增殖倍数，Hoechst染色检测细胞增殖，体外侵袭实验检测细胞侵袭，蛋白质印迹检测Ki67、VEGF、MMP-2和cleaved caspase-3的表达。结果　miR-143-3p mimic抑制人结肠癌细胞SW620中MAPK1 mRNA和蛋白的表达水平；miR-143-3p mimic与MAPK1野生型报告载体共转后，荧光素酶的活性显著降低；miR-143-3p mimic转染SW620细胞后，细胞增殖、侵袭能力显著降低，凋亡细胞数目显著增加，Ki67、VEGF和MMP-2的表达水平显著降低，cl-caspase-3的表达水平显著上升；miR-143-3p mimic能缓解MAPK1高表达对SW620细胞增殖、侵袭的诱导作用和对细胞凋亡的抑制作用。结论　miR-143-3p抑制人结肠癌细胞增殖和侵袭，诱导细胞凋亡，其作用机制与靶向抑制MAPK1有关。

Abstract

Objective　To investigate the relationship between miR-143-3p targeting MAPK1 and its effect on proliferation, apoptosis and invasion of human colon cancer cells.　Methods　qRT-PCR was used to detect the effect of transfection and the expression level of MAPK1 mRNA. Double luciferase reporter assay was used to detect the targeting relationship between microRNAs-143-3p and MAPK1. The expression level of MAPK1 protein was detected by Western blot. The proliferation multiple was detected by CCK-8. The proliferation was detected by Hoechst staining. The cell invasion was detected by in vitro invasion test. The expression of Ki67, VEGF，MMP-2 and claspase-3 was detected by Western blot.　Results　miR-143-3p mimic inhibited the expression of MAPK1 mRNA and protein in SW620 cells. miR-143-3p mimic and MAPK1 wild-type reporter plasmid decreased the activity of luciferase significantly. After miR-143-3p mimic transfection, the proliferation and invasion of SW620 cells were significantly decreased, the number of apoptotic cells was significantly increased, the expression of Ki67, VEGF and MMP-2 was significantly decreased, and the expression of cl-caspase-3 was significantly increased. The miR-143-3p mimic alleviated the induction effect of high expression of MAPK1 on proliferation and invasion of SW620 cells and inhibition effect on cell apoptosis.　Conclusion　miR-143-3p can inhibit proliferation and invasion of human colon cancer cells and induce apoptosis through possible targeting inhibition of MAPK1.

导出引用

张志谦, 张广星, 彭小东. miR-143-3p 靶向MAPK1对人结肠癌细胞增殖，凋亡和侵袭的调节作用#br#[J]. 中国临床解剖学杂志. 2019, 37(3): 292-298 https://doi.org/10.13418/j.issn.1001-165x.2019.03.011

ZHANG Zhi-qian, ZHANG Guang-xin, PENG Xiao-dong. The regulatory effects of miR-143-3p on proliferation, apoptosis and invasion on human colon cancer cell via targeting MAPK1[J]. Chinese Journal of Clinical Anatomy. 2019, 37(3): 292-298 https://doi.org/10.13418/j.issn.1001-165x.2019.03.011

中图分类号： R735.3　

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