多巴胺D2受体基因敲除导致体重减轻以及棕色脂肪PRDM16、UCP1、FAS和ACC的表达变化#br#

doi:10.13418/j.issn.1001-165x.2019.03.008

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (3): 278-281.doi: 10.13418/j.issn.1001-165x.2019.03.008

多巴胺D2受体基因敲除导致体重减轻以及棕色脂肪PRDM16、UCP1、FAS和ACC的表达变化#br#

张毅敏^{1, 2, 3}，　连辉^{1, 2, 3}，　付升旗^{1, 2, 3}，　宋海岩^{1, 2, 3}，　毛康⁴

1.新乡医学院基础医学院；　2.河南省医用组织再生重点实验室；　3.河南省分子诊断与医学检验技术协同创新中心；
4.新乡医学院三全学院，河南新乡 453003

收稿日期:2018-10-17 出版日期:2019-05-25 发布日期:2019-06-13
通讯作者: 宋海岩，副教授，E-mail：shy80825@163.com
作者简介:张毅敏（1987-），实验师，研究方向：解剖生理学，E-mail：zhangyimin2012123@126.com
基金资助:
国家自然科学基金青年科学基金项目（81600677）; 新乡医学院博士科研基金（XYBSKYZZ201815）

Changes of PRDM16, UCP1, FAS and ACC expression of dopamine D2 receptor knockout mice

ZHANG Yi-min^{1, 2, 3}, LIAN Hui^{1, 2, 3}, FU Sheng-qi^{1, 2, 3}, SONG Hai-yan^{1, 2, 3}, MAO Kang⁴

1. College of Basic Medical Science, Xinxiang Medical College; 2. Henan Key Laboratory of Medical Tissue Regeneration; 3. Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine; 4.Sanquan Medical college, Xinxiang 453003, Henan Province, China

Received:2018-10-17 Online:2019-05-25 Published:2019-06-13

摘要/Abstract

摘要： 目的　以多巴胺D2受体基因敲除（D2KO）小鼠为动物模型，研究D2KO小鼠体重减轻后，棕色脂肪组织（brown adipose tissue，BAT）重量，白色脂肪重量，BAT中含PR结构域蛋白16（PRD1-BF1-RIZ1 homologous domain containing 16，PRDM16）、解偶联蛋白1（uncoupling protein 1，UCP1）、脂肪酸合酶（fatty acid synthase，FAS）以及乙酰辅酶A羧化酶（acetyl Coenzyme A carboxylase，ACC）的mRNA和蛋白表达变化，以期为肥胖机制研究提供线索。方法　8周龄野生型小鼠组（wild type，WT）和D2KO小鼠组各10只。分别称量各小鼠体重及其白色脂肪、棕色脂肪重量。Real-time quantitative RT-PCR方法检测棕色脂肪中PRDM16、UCP1、FAS、ACC的mRNA表达水平，Western blotting方法检测棕色脂肪中PRDM16、UCP1、FAS、ACC的蛋白表达水平。结果　WT组动物体重为（23.78±0.54）g，D2KO组动物体重为（20.54±1.15）g，P＜0.05。WT组动物附睾脂肪（epididymal adipose tissue，eAT）重量为（1.083±0.02）g，D2KO组动物eAT重量为（0.978±0.03）g，P＜0.05。WT组动物皮下脂肪（subcutaneous adipose tissue，sAT）重量为（1.85±0.05）g，D2KO组动物sAT重量为（1.72±0.03）g，P<0.05。WT组动物BAT重量为（31.38±4.22）mg，D2KO组动物BAT重量为（42.44±2.47）mg，P<0.05。半定量PCR与Western blotting检测结果显示，相对于WT组，D2KO组小鼠棕色脂肪中PRDM16、FAS、ACC的mRNA与蛋白的表达均显著增加，UCP1的mRNA与蛋白表达显著减少。结论　D2KO小鼠的体重减轻与棕色脂肪中的PRDM16、UCP1、FAS以及ACC存在一定关联。

关键词: D2受体敲除, 　体重减轻, 　棕色脂肪

Abstract: Objective　To observe the expression of PRD1-BF1-RIZ1 homologous domain containing 16 (PRDM16), uncoupling protein 1 (UCP1), fatty acid synthase (FAS) and acetyl Coenzyme A carboxylase (ACC) in dopamine D2 receptor knockout mice.　Methods　The body weight and fat mass of wild type group (WT) and dopamine D2 receptor knockout group (D2KO) were compared. Real-time quantitative RT-PCR and Western blotting analysis were used to detect PRDM16, UCP1, FAS and ACC expression in BAT of both groups.　Results　Body weight of the WT group was (23.78±0.54) g while D2KO group was (20.54±1.15) g. The epididymal adipose tissue (eAT) fat mass was (1.083±0.02) g while the D2KO group was (0.978±0.03) g. The subcutaneous adipose tissue (sAT) fat mass was (1.85±0.05) g while the D2KO group was (1.72±0.03) g. The brown adipose tissue (BAT) fat mass was (31.38±4.22) mg while the D2KO group was (42.44±2.47) mg. Compared with the WT group, the mRNA and protein expression of UCP1 in BAT were significantly decreased in D2KO group while the mRNA and protein expression of PRDM16, FAS, ACC were significantly increased. The mRNA and protein level of ACC did not change between two groups. Conclusion　The expression of PRDM16, UCP1, FAS and ACC in BAT may be related to the weight loss of dopamine D2 receptor knockout mice. Compared with the WT group, the mRNA and protein expression of UCP1 in BAT is significantly decreased in D2KO group while the mRNA and protein expression of PRDM16, FAS, ACC is significantly increased.

Key words: Dopamine D2 receptor knockout mice, 　Weight loss, 　Brown adipose tissue

中图分类号:

R322.85 R354

张毅敏, 连辉, 付升旗, 宋海岩, 毛康. 多巴胺D2受体基因敲除导致体重减轻以及棕色脂肪PRDM16、UCP1、FAS和ACC的表达变化#br#[J]. 中国临床解剖学杂志, 2019, 37(3): 278-281.

ZHANG Yi-min, LIAN Hui, FU Sheng-qi, SONG Hai-yan, MAO Kang. Changes of PRDM16, UCP1, FAS and ACC expression of dopamine D2 receptor knockout mice[J]. Chinese Journal of Clinical Anatomy, 2019, 37(3): 278-281.

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多巴胺D2受体基因敲除导致体重减轻以及棕色脂肪PRDM16、UCP1、FAS和ACC的表达变化#br#

Changes of PRDM16, UCP1, FAS and ACC expression of dopamine D2 receptor knockout mice

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