葛根素对臂丛根性撕脱后脊髓MMP-9蛋白表达的影响

doi:10.13418/j.issn.1001-165x.2019.02.007

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (2): 148-152.doi: 10.13418/j.issn.1001-165x.2019.02.007

葛根素对臂丛根性撕脱后脊髓MMP-9蛋白表达的影响

陈传奇^1,2，　陈龙菊^1，3，　吴太鼎¹，　谭云霞¹，　李丹¹，　王雨¹

1. 湖北民族大学医学院解剖教研室；　2. 湖北民族大学附属恩施州土家族苗族自治州中心医院妇产科；
3. 风湿性疾病发生与干预湖北省重点实验室，湖北恩施 445000

收稿日期:2017-10-26 出版日期:2019-03-25 发布日期:2019-04-29
通讯作者: 陈龙菊，教授，博士，Tel: 0718-8437479, E-mail: clj1028@163.com
作者简介:陈传奇（1989-），住院医师，硕士，主要从事中西医结合防治周围神经损伤方面的研究，E-mail: 740224783@qq.com
基金资助:
国家自然科学基金（No.81260192）；湖北省教育厅项目（No.D20151903）

Effect of Puerarin on the expression of MMP-9 protein in spinal cord after brachial plexus root avulsion

CHEN Chuan-qi ^1，2, CHEN Long-ju^1,3, WU Tai-ding ¹, TAN Yun-xia¹, LI Dan ¹, WANG Yu¹

1.Department of Anatomy, Medical College, Hubei Minzu University；2.Obstetrics and Gynecology Department, Affiliated the Central Hospital of ENSHI Tujia and Miao Autonomous Prefecture, Hubei Minzu University; 3.Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Disease, Enshi 445000, Hubei Province, China

Received:2017-10-26 Online:2019-03-25 Published:2019-04-29

摘要/Abstract

摘要：

目的　探讨大鼠臂丛根性撕脱后脊髓基质金属蛋白酶-9（MMP-9）蛋白的表达变化以及葛根素对其表达的影响。方法　成年雄性SD大鼠174只，随机分为正常组、模型组、葛根素低、中、高处理组。模型组和葛根素处理组行右侧C5~C7脊神经前根撕脱术，术后腹腔注射给药。Western Blot法检测C5~C7节段脊髓MMP-9蛋白的表达。结果　与正常组比较，臂丛神经根性撕脱后1 d时MMP-9蛋白表达达高峰，3 d降至接近正常，1周后下降至较低水平。葛根素处理组脊髓组织MMP-9蛋白表达在1 d时比模型组显著降低。结论　MMP-9可能参与臂丛根性撕脱后早期损伤反应，且葛根素可能有助于减轻该种损伤。

关键词: 葛根素, 　臂丛, 　神经损伤, 　基质金属蛋白酶-9

Abstract:

Objective To investigate the expression changes of matrix metalloproteinase-9 (MMP-9) in spinal cord after brachial plexus root avulsion and the effect of Puerarin on its expression. Methods A total of 174 adult male SD rats were randomly divided into a normal group, a model group, Puerarin low, medium and high treatment groups. The model group and the Puerarin treatment groups underwent the right C5~C7 anterior nerve root avulsion. The rats were injected intraperitoneally Puerarin every day after the operation. The expression of MMP-9 protein in C5~C7 segment of spinal cord was detected by Western Blot. Results Compared with the normal group, the expression of MMP-9 protein peaked at the 1st day after brachial plexus root avulsion, decreased near to normal at the 3rd day and decreased to a lower level after 1 week. The expression of MMP-9 protein in spinal cord tissue of Puerarin treatment group was significantly lower than the model group at the 1st day. Conclusion MMP-9 may be involved in the early injury response to brachial plexus root avulsion, and Puerarin may help to alleviate this injury.

Key words: Puerarin, 　Brachial plexus, 　Nerve injury, 　Matrix metalloproteinase-9

陈传奇,陈龙菊,吴太鼎,谭云霞,李丹,王雨. 葛根素对臂丛根性撕脱后脊髓MMP-9蛋白表达的影响[J]. 中国临床解剖学杂志, 2019, 37(2): 148-152.

CHEN Chuan-qi, CHEN Long-ju, WU Tai-ding, TAN Yun-xia, LI Dan, WANG Yu . Effect of Puerarin on the expression of MMP-9 protein in spinal cord after brachial plexus root avulsion[J]. Chinese Journal Of Clinical Anatomy, 2019, 37(2): 148-152.

参考文献 23

[1]	Li W, Wang S, Zhao J, et al. Complications of contralateral C-7 transfer through the modified prespinal route for repairing brachial plexus root avulsion injury: a retrospective study of 425 patients[J]. J Neurosurg, 2015, 122(6): 1421-1428.
[2]	Vanaclocha V, Herrera JM, Verdu-Lopez F, et al. Transdiscal C6-C7 contralateral C7 nerve root transfer in the surgical repair of brachial plexus avulsion injuries[J]. Acta Neurochir (Wien), 2015, 157(12): 2161-2167.
[3]	Teles RM, Antunes SL, Jardim MR, et al. Expression of metalloproteinases (MMP-2, MMP-9, and TACE) and TNF-alpha in the nerves of leprosy patients[J]. J Peripher Nerv Syst, 2007, 12(3): 195-204.
[4]	Chattopadhyay S, Myers RR, Janes J, et al. Cytokine regulation of MMP-9 in peripheral glia: implications for pathological processes and pain in injured nerve[J]. Brain Behav Immun, 2007, 21(5): 561-568.
[5]	Shiying L, Xinhui Q, Guanghua J, et al. Puerarin promoted proliferation and differentiation of dopamine-producing cells in Parkinson's animal models[J].Biomed Pharmacother, 2018, 106: 1236-1242.
[6]	Liu B, Zhao C, Li H, et al. Puerarin protects against heart failure induced by pressure overload through mitigation of ferroptosis[J]. Biochem Biophys Res Commun, 2018, 497(1): 233-240.
[7]	Xie HT, Xia ZY, Pan X, et al. Puerarin ameliorates allodynia and hyperalgesia in rats with peripheral nerve injury[J]. Neural Regen Res, 2018,13(7): 1263-1268.
[8]	Chen L J, Ren YH, Liu L, et al. Upregulated expression of GAP-43 mRNA and protein in anterior horn motoneurons of the spinal cord after brachial plexus injury[J]. Arch Med Res, 2010, 41(7): 513-518.
[9]	Zhong Y, Huang YL, Hu YM, et al. Puerarin alleviate radicular pain from lumbar disc herniation by inhibiting ERK-dependent spinal microglia activation[J].Neuropeptides, 2018, 72: 30-37.
[10]	Inciong JG, Marrocco WC, Terzis JK, et al. Efficacy of intervention strategies in a brachial plexus global avulsion model in the rat[J]. Plast Reconst Surgr, 2000, 105(6): 2059-2071.
[11]	Zhou LH, Wu W. Survival of injured spinal motoneurons in adult rat upon treatment with glial cell line-derived neurotrophicfactor at 2 weeks but not at 4 weeks after root avulsion[J]. J Neurotrauma, 2006, 23(6): 920-927.
[12]	陈传奇. 葛根素对臂丛根性撕脱后脊髓前角运动神经元NGF、GAP-43mRNA及蛋白表达的影响[D]. 恩施: 湖北民族学院, 2017: 20-25.
[13]	盛华均, 孙善全. c-myc、MMP-9在受压静脉管壁的表达[J]. 中国临床解剖学杂志, 2009, 27(4): 448-451.
[14]	张同星, 井万里, 张涛, 等. 神经调节素对缺血再灌注损伤大鼠脊髓MMP-9和TIMP-1表达的影响[J]. 中国脊柱脊髓杂志, 2016, 26(7): 642-649.
[15]	Zhang P,Ma X. Effect of rutin on spinal cord injury through inhibition of the expression of MIP-2 and activation of MMP-9, and downregulation of Akt phosphorylation[J]. Mol Med Rep, 2015, 12(5): 7554-7560.
[16]	Yu F,Kamada H,Niizuma K, et al. Induction of mmp-9 expression and endothelial injury by oxidative stress after spinal cord injury[J]. J Neurotrauma, 2008, 25(3): 184-195.
[17]	Bakos SR,Costanzo RM. Matrix metalloproteinase-9 is associated with acute inflammation after olfactory injury[J]. Neuroreport, 2011, 22(11): 539-543.
[18]	Girard S, Sébire G,Kadhim H. Proinflammatory orientation of the interleukin 1 system and downstream induction of matrix metalloproteinase 9 in the pathophysiology of human perinatal white matter damage[J]. J Neuropathol Exp Neurol, 2010, 69(11): 1116-1129.
[19]	周华, 刘华. 脊髓损伤后MMP-9 表达的时空分布及其意义[J]. 创伤外科杂志, 2005, 7(3): 175-177.
[20]	Gundogdu EB,Bekar A,Turkyilmaz M, et al. CDP-choline modulates matrix metalloproteinases in rat sciatic injury[J]. J Surg Res, 2016, 200(2): 655-663.
[21]	Tanaka T,Moriyama T,Kawamura Y, et al. Puerarin Suppresses Macrophage Activation via Antioxidant Mechanisms in a CaPO4-Induced Mouse Model of Aneurysm[J]. J Nutr Sci Vitaminol (Tokyo), 2016, 62(6): 425-431.
[22]	Saraswat G,Guha R,Mondal K, et al. Molecular cues to the anti-implantation effect of nano-puerarin in rats[J]. Reproduction, 2016, 151(6): 693-707.
[23]	Tian F, Xu LH, Zhao W, et al. The neuroprotective mechanism of puerarin treatment of acute spinal cord injury in rats[J]. Neurosci Lett, 2013, 543: 64-68.

葛根素对臂丛根性撕脱后脊髓MMP-9蛋白表达的影响

Effect of Puerarin on the expression of MMP-9 protein in spinal cord after brachial plexus root avulsion

可视化

摘要/Abstract

引用本文

使用本文

参考文献 23

相关文章 5

Metrics

本文评价

推荐阅读 0

[1]	李咪, 许嘉玮, 邹颖, 郭家松. 小鼠臂丛离断对脊髓运动神经元树突结构与形态退变的影响[J]. 中国临床解剖学杂志, 2022, 40(3): 277-285.
[2]	詹翼, 贝朝涌. 儿童肱骨髁上骨折相关的神经损伤研究进展[J]. 中国临床解剖学杂志, 2021, 39(1): 115-117.
[3]	王雨, 陈传奇, 王文晟, 喻保军, 张义, 李丹, 陈龙菊. 葛根素对臂丛神经根性撕脱伤脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响[J]. 中国临床解剖学杂志, 2019, 37(5): 517-522.
[4]	吴昀,许云飞,贺细菊,张文君,贺赟,蹇梦婵,李文春. 葛根素对自发性高血压大鼠肠系膜动脉舒张功能的影响[J]. 中国临床解剖学杂志, 2019, 37(2): 142-147.
[5]	黄伟奇，　杨晓东，　李涛. 骶髂关节周围腰骶丛神经的解剖学研究及其临床意义[J]. 中国临床解剖学杂志, 2017, 35(6): 615-617.