帕金森病猴模型生化指标及肾上腺的改变

doi:10.13418/j.issn.1001-165x.2019.01.011

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (1): 51-54.doi: 10.13418/j.issn.1001-165x.2019.01.011

帕金森病猴模型生化指标及肾上腺的改变

田荆华¹，　朱明慧¹，　张争辉¹，　于兰²

1.菏泽医学专科学校人体解剖学教研室，山东菏泽 274000； 2.桂林医学院人体解剖学教研室，广西桂林 541000

收稿日期:2018-08-13 出版日期:2019-01-25 发布日期:2019-02-20
通讯作者: 于兰，副教授，硕士生导师， E-mail: yulan_113@163.com
作者简介:田荆华(1978-)，女，副教授，研究方向：帕金森病、内分泌与代谢、中医药与肿瘤，E-mail: tianjinghua_66@126.com
基金资助:
国家自然科学基金面上项目（81471054）；广西研究生教育创新计划项目 (JGY2015128)；广西青年科学基金项目(2017GXNSFBA198019)；广西脑与认知神经科学重点实验室开放课题（2017-6）；山东省高等学校科技计划项目(J13LK20)

The changes of adrenal gland and biochemical indicators in cynomolgus monkey models of Parkinson’s disease

TIAN Jing-hua¹，ZHU Ming-hui¹，ZHANG Zheng-hui¹， YU Lan²

1. Department of Human Anatomy, Heze Medical College, Heze 274000, Shandong Province, China；2. Department of Human Anatomy, Guilin Medical University, Guilin 541004, Guangxi Province, China

Received:2018-08-13 Online:2019-01-25 Published:2019-02-20

摘要/Abstract

摘要：

目的　探究食蟹猴1-甲基- 4-苯基-1,2,3,6-四氢吡啶(MPTP )慢性帕金森病（parkinson’s disease，PD）造模后生化指标、肾上腺组织的改变。方法　将8只雄性食蟹猴随机分为两组，对照组3只，造模组5只，用MPTP造模30 d。记录观察两组的行为学变化。食蟹猴造模成功后，抽血检测肾功能、葡萄糖、胆固醇、甘油三酯。动物麻醉安乐死后，取肾上腺组织，进行HE、α-突触核蛋白(α-synuclein)、多巴胺受体D2染色并观察病理学改变。结果　食蟹猴PD模型成功建立模型组和对照组比较，生化指标葡萄糖（glucose，GLU）、胆固醇（cholesterol，CH）有统计学差异（P<0.05），肾功能无明显差异。模型组肾上腺组织有损害，可见炎性细胞浸润，被膜与各带、髓质之间分界基本不清，皮质层变薄，部分呈局灶性坏死。α-synuclein在肾上腺的表达明显高于对照组，而髓质区多巴胺受体D2明显减少，免疫组化半定量分析，两组有统计学差异（P<0.05）。结论　食蟹猴PD模型代谢指标明显下降；其肾上腺结构功能的改变可能与PD发病有一定的相关性；通过检测外周生物学指标可为PD前期诊断提供新思路。

关键词: 帕金森病, 　肾上腺, 　肾功能, 　多巴胺受体, 　&alpha, -synuclein

Abstract:

Objective　To investigate the changes of biochemical indicators and adrenal glands after chronic MPTP treatment in cynomolgus monkeys. Methods　Eight male cynomolgus monkeys aged about 10 years old were randomly divided into two groups: a control group (n=3) and a model group (n=5) receiving MPTP administration for 30 days. The animal behavior was observed and recorded during animal modelling. After successful cynomolgus monkey modelling, blood was drawn to measure the kidney function, glucose, cholesterol, and triglycerides. After anaerobic animal anesthesia, the adrenal gland was taken for HE, α-synuclein, dopamine receptor D2 staining and pathological changes were observed.　Results　The PD model was successfully established in cynomolgus monkeys. There were significant differences in the biochemical indexes of glucose(GLU) and cholesterol(CH) between the groups (P<0.05). There was no significant difference in renal function. The adrenal gland was damaged. The inflammatory cells infiltrated. The boundary between the capsule and medulla was basically unclear. The cortical layer became thinner, and some of it underwent focal necrosis. The expression of α-synuclein in the adrenal gland was significantly higher than that in the control group, while the dopamine receptor D2 in the medulla was significantly reduced. There were statistically significant differences between the two groups in immunohistochemical semi-quantitative analysis(P<0.05).　Conclusions　The metabolic index of cynomolgus PD model is significantly decreased. The structural function of adrenal gland may be related to the pathogenesis of PD. The detection of peripheral biological indicators provides a new idea for the diagnosis of PD.

Key words: Parkinson's disease； , Adrenal gland；　Renal function；　Dopamine receptor, α-synuclein

田荆华,朱明慧,张争辉,于兰. 帕金森病猴模型生化指标及肾上腺的改变[J]. 中国临床解剖学杂志, 2019, 37(1): 51-54.

TIAN Jing-hua，ZHU Ming-hui，ZHANG Zheng-hui， YU Lan. The changes of adrenal gland and biochemical indicators in cynomolgus monkey models of Parkinson’s disease[J]. Chinese Journal Of Clinical Anatomy, 2019, 37(1): 51-54.

参考文献

[1] Chen-Plotkin AS. Parkinson disease: blood transcriptomics for parkinson disease[J]. Nat Rev Neurol, 2018, 14(1): 5-6.
[2] Al-Jarrah MD, Erekat NS. Parkinson disease-induced upregulation of apoptotic mediators could be attenuated in the skeletal muscle following chronic exercise training[J]. NeuroRehabilitation, 2017, 41(4): 823-830.
[3] Mendes Filho D, Ribeiro PDC, Oliveira LF, et al. Therapy with mesenchymal stem cells in parkinson disease: history and perspectives[J]. Neurologist, 2018, 23(4): 141-147.
[4] Xie F, Gao X, Yang W, et al. Advances in the research of risk factors and prodromal biomarkers of parkinson's disease[J]. ACS Chem Neurosci, 2018, 16(1): 561-572.
[5] 薛毅珑, 王振福, 李新建, 等. 微囊化牛肾上腺髓质嗜铬细胞脑内移植治疗偏侧帕金森病样大鼠及猴的实验研究[J]. 解放军医学杂志, 1999, 24(4): 7-10.

[6] 杨巍巍, 李昕, 李旭冉, 等. 寡聚化和磷酸化α-突触核蛋白在不同年龄段食蟹猴脑不同部位突触小体中的表达分布[J]. 首都医科大学学报, 2015, 36(5): 671-674.　　　　　　　　　　
[7] Imbert C, Bezard E, Guitraud S, et al. Comparison of eight clinical rating scales used for the assessment of MPTP-induced parkinsonism in the Macaque monkey[J]. J Neurosci Methods, 2000, 96(1): 71-76.
[8] 庞书涛, 朱明慧, 金兴山, 等. 帕金森病治疗的研究进展[J]. 广东医学, 2016, 37(21): 3290-3292.
[9] 李旭冉, 杨巍巍, 李昕, 等. α-突触核蛋白在食蟹猴消化道不同部位的表达[J]. 首都医科大学学报, 2015, 36(5): 675-679.
[10]Small DM. Dopamine Adaptations as a Common Pathway for Neurocognitive Impairment in Diabetes and Obesity: A Neuropsychological Perspective[J]. Front Neurosci, 2017, 11(1): 134-139.
[11]Phillips MCL, Murtagh DKJ, Gilbertson LJ, et al. Low-fat versus ketogenic diet in Parkinson's disease: a pilot randomized controlled trial[J]. Mov Disord, 2018, 33: 1306-1314.
[12]Emelyanov A, Andoskin P, Pchelina S. Dataset of total, oligomeric alpha-synuclein and hemoglobin levels in plasma in Parkinsons disease[J]. Data Brief, 2017, 10(1): 182-185.
[13] Peng C, Gathagan RJ, Covell DJ, et al. Cellular milieu imparts distinct pathological alpha-synuclein strains in alpha-synucleinopathies[J]. Nature, 2018, 557(7706): 558-563.
[14]吕捷, 张进禄, 赵春礼. 牛嗜铬细胞体外培养及其分泌多巴胺的测定[J].首都医科大学学报, 2000, 21(3): 185-186.
[15]刘振国. 肾上腺髓质移植治疗帕金森病临床尝试的研究进展[J]. 国外医学(老年医学分册), 1999,20(2): 76-81.
[16]Masellis M, Collinson S, Freeman N, et al. Dopamine D2 receptor gene variants and response to rasagiline in early Parkinson's disease: a pharmacogenetic study[J]. Brain, 2016, 139(Pt 7): 2050-2062.
[17] 林昶, 陈伟, 柴艺汇, 等. 基于下丘脑-垂体-肾上腺皮质轴探讨维生素D与肾虚证的关系[J]. 辽宁中医杂志, 2017, 44(7): 1403-1404.

帕金森病猴模型生化指标及肾上腺的改变

The changes of adrenal gland and biochemical indicators in cynomolgus monkey models of Parkinson’s disease

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