帕金森病猴模型生化指标及肾上腺的改变
The changes of adrenal gland and biochemical indicators in cynomolgus monkey models of Parkinson’s disease
目的 探究食蟹猴1-甲基- 4-苯基-1,2,3,6-四氢吡啶(MPTP )慢性帕金森病（parkinson’s disease，PD）造模后生化指标、肾上腺组织的改变。 方法 将8只雄性食蟹猴随机分为两组，对照组3只，造模组5只，用MPTP造模30 d。记录观察两组的行为学变化。食蟹猴造模成功后，抽血检测肾功能、葡萄糖、胆固醇、甘油三酯。动物麻醉安乐死后，取肾上腺组织，进行HE、α-突触核蛋白(α-synuclein)、多巴胺受体D2染色并观察病理学改变。 结果 食蟹猴PD模型成功建立模型组和对照组比较，生化指标葡萄糖（glucose，GLU）、胆固醇（cholesterol，CH）有统计学差异（P<0.05），肾功能无明显差异。模型组肾上腺组织有损害，可见炎性细胞浸润，被膜与各带、髓质之间分界基本不清，皮质层变薄，部分呈局灶性坏死。α-synuclein在肾上腺的表达明显高于对照组，而髓质区多巴胺受体D2明显减少，免疫组化半定量分析，两组有统计学差异（P<0.05）。 结论 食蟹猴PD模型代谢指标明显下降；其肾上腺结构功能的改变可能与PD发病有一定的相关性；通过检测外周生物学指标可为PD前期诊断提供新思路。
Objective To investigate the changes of biochemical indicators and adrenal glands after chronic MPTP treatment in cynomolgus monkeys. Methods Eight male cynomolgus monkeys aged about 10 years old were randomly divided into two groups: a control group (n=3) and a model group (n=5) receiving MPTP administration for 30 days. The animal behavior was observed and recorded during animal modelling. After successful cynomolgus monkey modelling, blood was drawn to measure the kidney function, glucose, cholesterol, and triglycerides. After anaerobic animal anesthesia, the adrenal gland was taken for HE, α-synuclein, dopamine receptor D2 staining and pathological changes were observed. Results The PD model was successfully established in cynomolgus monkeys. There were significant differences in the biochemical indexes of glucose(GLU) and cholesterol(CH) between the groups (P<0.05). There was no significant difference in renal function. The adrenal gland was damaged. The inflammatory cells infiltrated. The boundary between the capsule and medulla was basically unclear. The cortical layer became thinner, and some of it underwent focal necrosis. The expression of α-synuclein in the adrenal gland was significantly higher than that in the control group, while the dopamine receptor D2 in the medulla was significantly reduced. There were statistically significant differences between the two groups in immunohistochemical semi-quantitative analysis(P<0.05). Conclusions The metabolic index of cynomolgus PD model is significantly decreased. The structural function of adrenal gland may be related to the pathogenesis of PD. The detection of peripheral biological indicators provides a new idea for the diagnosis of PD.
帕金森病 / 肾上腺 / 肾功能 / 多巴胺受体 / &alpha / -synuclein
Parkinson's disease； / Adrenal gland； Renal function； Dopamine receptor / α-synuclein
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