神经肽Y在兔颈部交感神经节与脊神经节间通路应用研究

韩建龙,邱思强,朱新炜,栾方海,常刚,程继祖,左金良

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (6) : 621-625.

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (6) : 621-625. DOI: 10.13418/j.issn.1001-165x.2018.06.005
实验研究

神经肽Y在兔颈部交感神经节与脊神经节间通路应用研究

  • 韩建龙1, 邱思强1, 朱新炜1, 栾方海1, 常刚1, 程继祖2, 左金良1
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The study of neuropeptide Y in neural pathway between cervical spinal ganglia and cervical sympathetic ganglia in rabbit

  • HAN Jian-long1, QIU Si-qiang1, ZHU Xin-wei1, LUAN Fang-hai1, CHANG Gang1, CHENG Ji-zu2, ZUO Jin-liang1
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摘要

目的 通过电刺激兔颈脊神经节,检测颈交感神经节内的神经肽Y (neuropeptide Y,NPY)含量,研究颈交感神经节与颈脊神经节间的通路联系,探讨颈性眩晕发病机制中的神经解剖学基础。  方法 96只新西兰兔随机分为C2、C3、C4、C5、C6、C7组及相应对照组(每组8只),麻醉后显露相应的脊神经节,给予波宽0.5 ms、频率30 Hz、5 V电刺激,5秒/次,每1 min重复1次,共5次,30 min后,灌注固定,切取双侧颈上、下交感神经节,切片,免疫反应,染色,观察。  结果 电刺激C2、C3脊神经节后,同侧颈上交感神经节内NPY含量升高(P<0.05);电刺激C4、C5脊神经节后,同侧颈上交感神经节和颈下交感神经节内NPY含量均有明显变化(P<0.05);电刺激C6、C7脊神经节后,同侧颈下交感神经节内NPY含量升高(P<0.05)。  结论 颈脊神经节和颈交感神经节间存在神经电生理上的通路联系,且具有节段性、同侧性特点,为颈性眩晕的发病机制及临床分型提供了实验依据和理论基础。

Abstract

Objective To study the neural pathway between cervical spinal ganglia and cervical sympathetic ganglia, and to explore the neural factors in the pathogenesis of cervical vertigo by detecting the neuropeptide Y (NPY) in cervical sympathetic ganglia after the cervical spinal ganglia electro-stimulated.   Methods 96 New Zealand rabbits were divided into C2, C3, C4, C5, C6, C7 and control groups randomly (n=8 in each group ). The electro-stimulations (width=0.5 ms, 30 Hz, 5 V, time=5 s, 1 time/1 min, and 5 times total) were given to specific spinal ganglia which were revealed. After 30 min, the rabbits were killed through perfusion and the superior cervical sympathetic ganglia (SCG) and inferior cervical sympathetic ganglia (ICG) of both sides were cut. Then the section were made after immunologic reaction and observed under the microscope. Results The content of NPY in the same side sympathetic ganglia was higher than the other side sympathetic ganglia and the control group sympathetic ganglia after the electro-stimulation was given to the cervical spinal ganglia, and the difference was statistic significance (P<0.05). When the electro-stimulation was given to the C2, C3 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in SCG mainly; when the electro-stimulation were given to the C4, C5 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in SCG and ICG; when the electro-stimulation were given to the C6, C7 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in ICG mainly.   Conclusion There is some kind of neural pathway between the cervical spinal ganglia and cervical sympathetic ganglia, and the characteristic of segment and homonymy in this kind of connection is also found, which provides a neuroanatomical basis for the hypothesis of reflex arc in cervical vertigo.

关键词

颈脊神经节 /  颈交感神经节 /  神经肽Y(NPY) /  颈性眩晕 /  神经反射弧

Key words

Cervical spinal ganglion /  Cervical ganglion /  Neuropeptide Y /  Cervical vertigo /  Nervous reflex arc

引用本文

导出引用
韩建龙,邱思强,朱新炜,栾方海,常刚,程继祖,左金良. 神经肽Y在兔颈部交感神经节与脊神经节间通路应用研究[J]. 中国临床解剖学杂志. 2018, 36(6): 621-625 https://doi.org/10.13418/j.issn.1001-165x.2018.06.005
HAN Jian-long, QIU Si-qiang, ZHU Xin-wei, LUAN Fang-hai, CHANG Gang, CHENG Ji-zu, ZUO Jin-liang. The study of neuropeptide Y in neural pathway between cervical spinal ganglia and cervical sympathetic ganglia in rabbit[J]. Chinese Journal of Clinical Anatomy. 2018, 36(6): 621-625 https://doi.org/10.13418/j.issn.1001-165x.2018.06.005

参考文献

[1]  Li Y, Peng B. Pathogenesis, diagnosis, and treatment of cervical vertigo[J]. Pain Physician, 2015, 18(4): E583-E595.
[2]  左金良, 邱思强, 魏光伟, 等. 逆行标记法研究颈脊神经节至交感神经节的神经反射通路[J]. 解剖学报, 2011, 42(5): 615-618.
[3]  Zuo J, Han J, Qiu S, et al. Neural reflex pathway between cervical spinal and sympathetic ganglia in rabbits: implication for pathogenesis of cervical vertigo[J]. Spine J, 2014, 14(6): 1005-1009.
[4]  Zuo J, Han J, Qiu S, et al. Nerve fiber connection between the cervical spinal ganglia and cervical sympathetic ganglia: an in vivo anatomic study in rabbits[J]. J Hard Tissue Biology, 2013, 22(2): 211-214.
[5]  Yacovino DA, Hain TC. Clinical characteristics of cervicogenic-related dizziness and vertigo[J]. Semin Neurol, 2013, 33(3): 244-255.
[6] Perlmutter DH, Petraglia AL, Barbano R, et al. Microvascular decompression in patient with atypical features of hemifacial spasm secondary to compression by a tortuous vertebrobasilar system: case report[J]. Neurosurgery, 2010, 66(6): E1212.
[7] Ongun N, Atalay NS, Degirmenci E, et al. Tetra-ataxiometric posturography in patients with migrainous vertigo[J]. Pain Physician, 2016, 19(1): E87-E95.  
[8] Johnston JL, Daye PM, Thomson GT. Inaccurate saccades and enhanced vestibulo-ocular reflex suppression during combined eye-head movements in patients with chronic neck pain: possible implications for cervical vertigo [J]. Front Neurol, 2017, 8: 23.
[9]  van Leeuwen RB, van der Zaag-Loonen H. Dizziness and neck pain: a correct diagnosis is required before consulting a physiotherapist [J]. Acta Neurol Belg, 2017, 117(1): 241-244.
[10]  Al Saif AA, Al Senany S. Determine the effect of neck muscle fatigue on dynamic visual acuity in healthy young adults. J Phys Ther Sci, 2015, 27(1): 259-263.
[11]  Dieterich M. Dizziness[J]. Neurologist, 2004, 10(3): 154-164.
[12]  Lundberg JM, Tatemoto K. Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to alpha-adrenoceptor blockade[J]. Acta Physiol Scand, 1982, 116(4): 393-402.
[13]  Galli S, Naranjo A, Van Ryn C, et al. Neuropeptide Y as a biomarker and therapeutic target for neuroblastoma[J]. Am J Pathol, 2016, 186(11): 3040-3053.
[14] 康永生, 高延征. 颈性眩晕与神经肽Y相关性研究[J]. 中华实用诊断与治疗杂志, 2011(11): 1041-1042.

基金

国家自然基金项目(81472138)


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