关键词:  , 大鼠急性心衰模型, 　单羧酸转运蛋白2, 　大脑皮质

Abstract:

Objective　To observe the changes of monocarboxylate transporters 2( MCT2) expression in the rat cerebral cortex under the stimulation of acute heart failure syndrome, so as to detect the alterations of energy metabolism of the cerebral cortex under the condition of acute heart failure, and to provide experimental basis for the best rescue time of acute heart failure.　Methods　The model was established by intravenous injection of 1.5% pentobarbital sodium.To estimate whether acute heart failure model was successful or not, the left ventricular systolic pressure（LVSP）and increased rate of left ventricular pressure maximum（+dp/dt max）were recorded. Immunohistochemical staining(IHC) and Western blot(WB) were used to explore the alteration of MCT2 expression in rat cerebral cortex. 　Results　Compared with the normal group, the number and the integral optical density（IOD）of MCT2-positive neurons were increased in the cerebral cortex of the acute heart failure model，reaching the highest level on 5 min and declined on 10 min, but still higher than those in the control group (P<0.05).　Conclusion　Our results involved that MCT2 is enhanced under the case of acute heart failure, which might indicate that MCT2 is involved in the process of self-compensation in the absence of cerebral ischemia.

Key words: Acute heart failure model, 　MCT2, 　Cerebral cortex