导电弹性水凝胶促进C2C12细胞增殖的实验研究
Conductive and elastic cryogel for enhancing the proliferation of C2C12 cell
目的 本文主要探讨一种多巴胺激活的导电冷冻水凝胶(Dopamine-based polypyrrole -methacrylated gelatin cryogel, Ppy-GelMA)对成肌细胞活性的影响。 方法 通过扫描电镜了解材料结构、细胞粘附生长情况，并通过活死染色、CCK-8试验、Ki-67免疫荧光染色明确此水凝胶对C2C12细胞的影响。 结果 扫描电镜结果显示此水凝胶具有均一连通的多孔结构， Ppy-GelMA导电水凝胶上的成肌细胞具有同向排列性；活死染色显示此水凝胶对C2C12成肌细胞有良好的细胞相容性；CCK-8检测显示Ppy-GelMA导电水凝胶上的细胞活力显著高于GelMA水凝胶上的细胞活力； Ki67免疫染色结果显示Ppy-GelMA导电水凝胶相比GelMA水凝胶拥有更高的Ki67阳性细胞率。 结论 Ppy-GelMA导电水凝胶可促进成肌细胞增殖。
Objective In this study, the mussle-inspired polypyrrole (Ppy)-methacrylated gelatin (GelMA) cryogel（DOPA-base Ppy-GelMA） was investigated as a scaffold to enhance the proliferation of C2C12 cell. Methods The material structure and cell adhesion were investigated by scanning electron microscopy. The influence of cryogel on C2C12 cells was confirmed by live/dead staining, CCK-8 test and Ki-67 immunofluorescence staining. Result The Ppy-GelMA cryogel had the uniform and interconnected porous structure. Interestingly, myoblasts in Ppy-GelMA cryogel were oriented aligned. Both the GelMA cryogel and the Ppy-GelMA cryogel were suitable for the attachment and spread of C2C12 myoblasts, while the cell viability in Ppy-GelMA hcryogel was significantly better than that in GelMA cryogel. Furthermore, the Ki67 positive myoblasts rate in Ppy-GelMA cryogel was significantly better than that in GelMA cryogel. Conclusion This Ppy-GelMA cryogel can enhance the proliferation of C2C12 myoblasts.
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国家自然科学基金(31100700); 广东省医学科学技术研究基金(A2015412)
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