中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (3): 277-281.doi: 10.13418/j.issn.1001-165x.2018.03.009

• 实验研究 • 上一篇    下一篇

导电弹性水凝胶促进C2C12细胞增殖的实验研究

黄世嘉1, 2,    吴威乐2, 陈小星2, 王乐禹2, 刘成龙1   

  1. 1. 南方医科大学珠江医院创伤骨科,  广州   510220;    2. 南方医科大学人体解剖学教研室,
    广东省组织构建与检测重点实验室,  广州   510515
  • 收稿日期:2018-02-15 出版日期:2018-05-25 发布日期:2018-07-04
  • 通讯作者: 刘成龙,硕士生导师,副主任医师,E-mail:liucw878@163.com;王乐禹,副教授,E-mail:wangleyu889@163.com
  • 作者简介:黄世嘉(1990-),在读硕士,主要从事生物活性骨组织、骨骼肌组织修复材料的研究, Tel:15918702978,E-mail:285958738@qq.com
  • 基金资助:

    国家自然科学基金(31100700); 广东省医学科学技术研究基金(A2015412)

Conductive and elastic cryogel for enhancing the proliferation of C2C12 cell

HUANG Shi-jia 1, 2,WU Wei-le 2,CHEN Xiao-xing 2,WANG Le-yu 2,LIU Cheng-long 1   

  1. 1. Department of Orthopedic Trauma,Zhujiang Hospital, Southern Medical University, Guangzhou 510220, China; 2. Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China
  • Received:2018-02-15 Online:2018-05-25 Published:2018-07-04

摘要:

目的 本文主要探讨一种多巴胺激活的导电冷冻水凝胶(Dopamine-based polypyrrole -methacrylated gelatin cryogel, Ppy-GelMA)对成肌细胞活性的影响。  方法 通过扫描电镜了解材料结构、细胞粘附生长情况,并通过活死染色、CCK-8试验、Ki-67免疫荧光染色明确此水凝胶对C2C12细胞的影响。  结果 扫描电镜结果显示此水凝胶具有均一连通的多孔结构, Ppy-GelMA导电水凝胶上的成肌细胞具有同向排列性;活死染色显示此水凝胶对C2C12成肌细胞有良好的细胞相容性;CCK-8检测显示Ppy-GelMA导电水凝胶上的细胞活力显著高于GelMA水凝胶上的细胞活力; Ki67免疫染色结果显示Ppy-GelMA导电水凝胶相比GelMA水凝胶拥有更高的Ki67阳性细胞率。  结论 Ppy-GelMA导电水凝胶可促进成肌细胞增殖。

关键词: 冻胶,  成肌细胞,  聚吡咯,  组织工程

Abstract:

Objective In this study, the mussle-inspired polypyrrole (Ppy)-methacrylated gelatin (GelMA) cryogel(DOPA-base Ppy-GelMA) was investigated as a scaffold to enhance the proliferation of C2C12 cell. Methods The material structure and cell adhesion were investigated by scanning electron microscopy. The influence of cryogel on C2C12 cells was confirmed by live/dead staining, CCK-8 test and Ki-67 immunofluorescence staining. Result The Ppy-GelMA cryogel had the uniform and interconnected porous structure. Interestingly, myoblasts in Ppy-GelMA cryogel were oriented aligned. Both the GelMA cryogel and the Ppy-GelMA cryogel were suitable for the attachment and spread of C2C12 myoblasts, while the cell viability in Ppy-GelMA hcryogel was significantly better than that in GelMA cryogel. Furthermore, the Ki67 positive myoblasts rate in Ppy-GelMA cryogel was significantly better than that in GelMA cryogel.   Conclusion This Ppy-GelMA cryogel can enhance the proliferation of C2C12 myoblasts.

Key words: Cryogels, Myoblasts, Polypyrrole, Tissue engineering