新生期内毒素血症对大鼠卵巢发育的影响
屈丽华,胡崛,杨红霞,秦佳俊,李玥,李美香,莫中成,石金凤,黄远臻
中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (3) : 272-276.
新生期内毒素血症对大鼠卵巢发育的影响
Effect of neonatal endotoxemia on ovary development of rats
目的 观察大鼠新生期内毒素血症对卵巢发育的影响。 方法 选用新生健康雌性SD大鼠，随机分为对照组和处理组，处理组于生后第4~12 d期间，隔日皮下注射脂多糖(75 µg/kg)，复制内毒素血症模型，对照组在相同时间皮下注射等容生理盐水。动态观察两组大鼠体重、阴道开口时间、第一次动情期出现时间和动情周期，并于生后14 d、阴道开口日、77 d称取体重后，分批处死，取双侧卵巢，称重、计算脏器系数；常规石蜡制片，光镜观察卵巢组织病理变化。 结果 ①处理组大鼠, 体重、卵巢重量均下降，阴道开口时间和第一次动情期出现的时间均延迟；②处理组大鼠,近期可见卵巢组织中血管充血扩张、组织间隙水肿，远期可见卵巢中正常卵泡数目减少、闭锁卵泡增加、动情周期延长。 结论 新生期内毒素血症，可造成雌鼠青春期延迟，性周期紊乱，卵泡储备减少。
Objective To investigate the effects of endotoxemia in neonatal rats on ovarian development. Methods A total of 60 neonatal Sprague-Dawley female rats were used in this study. The litters were randomly divided into either LPS-treated or saline control groups. These rats were administered 75 μg/kg LPS or an equivolume of non-pyrogenic saline via subcutaneous injection on alternate day from postnatal days 4 to 12. All the rats were then fed conventionally and their body weight, the day of vaginal opening (dVO), the day of first vaginal estrus and estrous (sexual) cycle were monitored dynamically. The rats were sacrificed on postnatal day(PND)14，dVO and PND77, respectively after the weight was measured. bilateral ovaries were harvested and weighted for calculation of organic coefficient. Histological changes of ovaries were observed by means of conventional paraffin sectioning. Results ① LPS-treated rats exhibited a decrease in the weight of body and ovary, and a significant delay in both the day of vaginal opening and the day of first vaginal estrus. ②LPS treatment could cause vasocongestion and angiectasis, interstitial edema in the ovaries of female rats in the immediate term，and contributed to a decrease in normal follicles, an increased in atretic follicles and prolonged estrous cycle in the late period. Conclusion Neonatal endotoxemia may affect the growth and development of female rats, and result in a delayed puberty, sexual cycle disorder and a decreased follicular reserve in female rats.
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湖南省自然基金资助项目（2015JJ2128），衡阳市科技局基金资助项目（2012KJ24）
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