白藜芦醇治疗POI大鼠药物浓度筛选

李宁; 刘丽丽

doi:10.13418/j.issn.1001-165x.2018.02.017

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (2) : 206-210. DOI: 10.13418/j.issn.1001-165x.2018.02.017

实验研究

白藜芦醇治疗POI大鼠药物浓度筛选

李宁，刘丽丽

作者信息 +

Screening of drug concentration of resveratrol in POI rats

LI Ning , LIU Li-li

Author information +

文章历史 +

摘要

目的　通过观察不同浓度白藜芦醇治疗早发性卵巢功能不全（POI）成模大鼠的疗效，确定其治疗早发性卵巢功能不全大鼠的最佳药物浓度。方法　将90只5周龄SPF级SD健康雌性大鼠按随机数字表分为低剂量白藜芦醇灌胃治疗组(a组18只)、中剂量白藜芦醇灌胃治疗组(b组18只)、高剂量白藜芦醇灌胃治疗组(c组18只)、模型对照组(d组18只)、空白对照组(e组18只)。e组从造模前的动物中随机抽取；a、b、c、d组从成功造模（72只给予雷公藤多苷片50 mg·kg-1·d-1 灌胃14 d，根据观测指标确定早发性卵巢功能不全成模）的动物中随机分组获得。a、b、c组分别采用每日20、50、80 mg/kg 灌胃剂量，模型对照组采用0.5％羧甲基纤维素钠溶液灌胃。42 d后采用ELISA法检测大鼠血清性激素：抗苗勒管激素（AMH）、黄体生成素（LH）、卵泡刺激素（FSH）、雌二醇（E2）水平，HE染色镜检大鼠单位卵巢面积内成熟卵泡、闭锁卵泡和黄体数，评价其疗效。结果　不同浓度白藜芦醇灌胃治疗组POI大鼠较0.5％羧甲基纤维素溶液灌胃组POI大鼠血清中AMH、E2水平高（P<0.05），LH、FSH水平低（P<0.05）。不同浓度白藜芦醇灌胃治疗组POI大鼠较空白对照组大鼠血清中AMH、E2水平低（P<0.05），LH、FSH水平高（P<0.05）。与a、b、c、e组比较，d组卵巢内颗粒细胞层数，成熟卵泡数以及黄体数量少（P<0.05），闭锁卵泡数明显增加（P<0.05）。结论　不同浓度白藜芦醇灌胃治疗POI大鼠均有一定的疗效，50 mg/kg浓度效果最好，是最佳药物浓度。

Abstract

Objective　To observe the curative effect of different concentrations of resveratrol on rats with premature ovarian insufficiency (POI) and to determine the optimal drug concentration of resveratrol for treating premature ovarian insufficiency dysfunction in rats.　Methods　A total of 90 5-week-old SPF grade SD healthy female rats were randomly divided into a low-dose resveratrol group (18 rats in group a) and a middle-dose resveratrol group (18 rats in group b), a high dose resveratrol group (18 rats in group c), a model group (18 rats in group d) and a blank control group (18 rats in group e). Group a, group b, group c were administered with a daily dose of 20 mg/kg, 50 mg/kg, 80 mg/kg by gavage, respectively; the model control group was administered with 0.5% sodium carboxymethyl cellulose solution by gavage. ELISA was used to detect the level of serum sex hormone levels: anti-Mullerian hormone (AMH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2) level and HE staining of rats to observe unit ovarian area of mature follicles, atresia follicles and corpus luteum, evaluation of resveratrol efficacy.　Results　Rats treated with different concentration of resveratrol had a significantly increased level of serum AMH and E2 compared to rats treated with 0.5% carboxymethyl cellulose solution (P<0.05), LH and FSH levels were lower (P<0.05).The serum level of AMH and E2 in POI rats treated with resveratrol at different concentration were lower (P<0.05) and LH and FSH were higher (P<0.05) than those of rats in the blank control. Compared with rats in group a, b, c and e, the number of granulosa cells, mature follicles and corpus luteum in ovaries was smaller in group d (P<0.05); In contrast, the number of atresia follicles was significantly increased in group d(P<0.05). Conclusion　Intragastric treatment with different concentration of resveratrol of all have a certain effect on POI rats receiving, with 50 mg/kg showing the best result, and thus being the best drug concentration.

导出引用

李宁,刘丽丽. 白藜芦醇治疗POI大鼠药物浓度筛选[J]. 中国临床解剖学杂志. 2018, 36(2): 206-210 https://doi.org/10.13418/j.issn.1001-165x.2018.02.017

LI Ning,LIU Li-li. Screening of drug concentration of resveratrol in POI rats[J]. Chinese Journal of Clinical Anatomy. 2018, 36(2): 206-210 https://doi.org/10.13418/j.issn.1001-165x.2018.02.017

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