脑缺血再灌注损伤程度与自噬相关基因LC3、mTOR表达的相关性研究

李丹丹; 肖学进; 秦书俭; 包翠芬

doi:10.13418/j.issn.1001-165x.2018.02.012

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (2) : 182-186. DOI: 10.13418/j.issn.1001-165x.2018.02.012

实验研究

脑缺血再灌注损伤程度与自噬相关基因LC3、mTOR表达的相关性研究

李丹丹¹，　肖学进¹，　秦书俭²，　包翠芬³

作者信息 +

Correlation between the degree of cerebral ischemia-reperfusion injury and the expression of LC3 and mTOR

LI Dan-dan¹， XIAO Xue-jin¹， QIN Shu-jian²， BAO Cui-fen³

Author information +

文章历史 +

摘要

目的　探讨局灶性脑缺血再灌注损伤（cerebral ischemia reperfusion, CIR）程度与自噬微管相关蛋白轻链3抗体-Ⅱ（Autophagy microtubule associated protein light chain 3 antibody-Ⅱ，LC3-Ⅱ）、雷帕霉素靶蛋白抗体（Rapamycin target protein antibody，mTOR）表达的相关性。方法　采用线栓阻断法阻塞大脑中动脉2 h制作大鼠局灶性脑缺血再灌注模型；观察各组间的神经功能缺失评分、TTC梗死体积；应用免疫印迹、免疫荧光法检测大脑顶叶的LC3-Ⅱ、mTOR的表达。结果　TTC染色显示，梗死体积在缺血再灌24 h达高峰。WB、免疫荧光显示，LC3-Ⅱ在IR中表达增加，且在缺血再灌24 h表达最高；mTOR在缺血再灌24 h、48 h表达最低。相关性分析显示，TTC染色梗死体积与LC3-Ⅱ表达水平高度正相关。结论　脑缺血再灌注损伤程度可能与LC3、mTOR的表达水平相关。

Abstract

Objective　To investigate the correlation between the degree of focal cerebral ischemia-reperfusion injury and the expression of LC3-Ⅱ and mTOR.　Methods　The models of focal cerebral ischemia-reperfusion in rats were established by occlusion of the middle cerebral artery. Neurological deficit score and TTC infarct volume were observed among groups. The expressions of LC3-Ⅱ and mTOR in different groups were detected by immunoblotting and immunofluorescence. Results　Immunoblotting and immunofluorescence showed that LC3-Ⅱ had the highest expression in 24 hours after ischemia and reperfusion (IR 24 h), and mTOR was the lowest in IR 24h and IR 48 h. Correlation analysis showed that the infarcted volume of TTC was positively correlated with the expression level of LC3-II.　Conclusion 　IR may activate the autophagy of the parietal neurons of the brain by increasing the expression level of LC3-II and decreasing the expression level of mTOR. The degree of cerebral ischemia-reperfusion injury may be related to the expression level of LC3 and mTOR.

导出引用

李丹丹,肖学进,秦书俭,包翠芬. 脑缺血再灌注损伤程度与自噬相关基因LC3、mTOR表达的相关性研究[J]. 中国临床解剖学杂志. 2018, 36(2): 182-186 https://doi.org/10.13418/j.issn.1001-165x.2018.02.012

LI Dan-dan,XIAO Xue-jin,QIN Shu-jian,BAO Cui-fen. Correlation between the degree of cerebral ischemia-reperfusion injury and the expression of LC3 and mTOR[J]. Chinese Journal of Clinical Anatomy. 2018, 36(2): 182-186 https://doi.org/10.13418/j.issn.1001-165x.2018.02.012

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