含硫氨基酸对糖尿病大鼠窦房结损伤的保护作用

周立; 王庆志; 王志勇

doi:10.13418/j.issn.1001-165x.2017.06.014

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (6) : 661-664. DOI: 10.13418/j.issn.1001-165x.2017.06.014

实验研究

含硫氨基酸对糖尿病大鼠窦房结损伤的保护作用

周立¹，　王庆志²，　王志勇¹

作者信息 +

Protective role of sulphur-containing amino acids on sinoatrial node dysfunction in type 1 diabetes rat model

ZHOU Li¹, WANG Qing-zhi², WANG Zhi-yong¹

Author information +

文章历史 +

摘要

目的　探讨补充半胱氨酸或牛磺酸对糖尿病大鼠窦房结损伤的作用。方法　SD大鼠随机分为正常对照组（C）组、糖尿病（DM）组、糖尿病+半胱氨酸（DM+Cys）组和糖尿病+牛磺酸（DM+Tau）组。检测SOD和MDA含量；测定窦房结功能SNRT、CSNRT、SACT和IHR；检测窦房结SOD2、HO-1、NOX2、NOX4、HCN2、HCN4表达。结果　与C组比较，DM组血SOD降低，MDA升高；SNRT、CSNRT和SACT延长，IHR减慢；窦房结SOD2、HO-1、HCN2和HCN4表达降低，NOX2和NOX4表达升高（P<0.05）。与DM组比较，DM+Cys和DM+Tau组血SOD升高；IHR增加；窦房结SOD2、HO-1、HCN2和HCN4表达升高，NOX2和NOX4表达降低（P<0.05）。结论　补充Cys或Tau能降低STZ大鼠窦房结氧化应激损伤，增加HCN4和HCN2表达，改善窦房结功能障碍。

Abstract

Objective　To explore the effects of cysteine or taurine on sinoatrial node dysfunction in diabetic rats and the underlying mechanism.　Methods　SD rats were randomly divided into four groups:control group(C) diabetes mellits group(DM), diabetes mellits+cysteine group(DM+Cys), diabetes mellits+taurine group(DM+Tau). The blood content of SOD and MDA were assessed. The sinoatrial node function was detected by SNRT, CSNRT, SACT and IHR. We also measured the expression of SOD2, HO-1, Nox2, Nox4, HCN2 and HCN4.　Results 　Compared with C group, the levels of SOD decreased, whereas MDA increased in DM group. The IHR was reduced with the prolonging of SNRT, CSNRT and SACT. The DM group exhibited down-regulated expression of SOD2, HO-1, HCN2 and HCN4 but up-regulated expression of NOX2 and NOX4 (P<0.05). Compared with DM group, DM+Cys and DM+Tau group showed increased SOD, prolonged IHR, up-regulated expression of SOD2, HO-1, HCN2 and HCN4, down-regulated expression of NOX2 and NOX4 (P<0.05). 　Conclusion　Cysteine or taurine supplementation can reduce the oxidative stress, increased the expression of HCN2 and HCN4, improve the sinoatrial node dysfunction in diabetic rats.

导出引用

周立,王庆志,王志勇. 含硫氨基酸对糖尿病大鼠窦房结损伤的保护作用[J]. 中国临床解剖学杂志. 2017, 35(6): 661-664 https://doi.org/10.13418/j.issn.1001-165x.2017.06.014

ZHOU Li, WANG Qing-zhi, WANG Zhi-yong. Protective role of sulphur-containing amino acids on sinoatrial node dysfunction in type 1 diabetes rat model[J]. Chinese Journal of Clinical Anatomy. 2017, 35(6): 661-664 https://doi.org/10.13418/j.issn.1001-165x.2017.06.014

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