LSCI检测不同缺血期对大鼠缺血再灌注过程脑血流的影响
Effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow detected by LSCI
目的 考察不同缺血期对大鼠缺血再灌注过程脑血流的影响。 方法 通过激光散斑成像技术（LSCI）检测大鼠大脑中动脉不同栓塞时间后，对再灌注过程中脑血流的影响，并对再灌注过程中的低灌注状态、无复流现象、血流流速及管径变化进行研究分析。 结果 2 h的栓塞后，大鼠脑部血流量趋向低灌注状态，约为栓塞前血流基值的(35±10)%；栓塞时间越长，无复流现象越多；在再灌注期间，缺血2 h组的血流比0.5 h组的低灌注水平低10%。 结论 长时间的脑缺血期再灌注过程可能是造成脑损伤程度加重的主要原因之一。LSCI可应用于脑部血流实时监测，操作简便，成像灵敏、稳定，结果可靠。
Objective To investigate the effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow. Methods Laser speckle imaging system (LSCI) was used to detect the effects of different ischemic duration on post-ischemia and reperfusion rat cerebral blood flow. At the same time, the dynamic changes of capillary flow and diameter, no reflow phenomenon and hypoperfusion were analyzed. Results During 2h middle cerebral artery occlusion (MCAO) ischemia, perfusion of cerebral blood flow decreased to (35±10)% of the baseline, reflecting a state of hypoperfusion. With the prolongation of ischemic time, no reflow phenomenon was aggravated. Hypoperfusion in 2 h group was approximately 10% less than that in 30 min group during the period of ischemia and reperfusion. Conclusion The long ischemic duration on post-ischemic and reperfusion may be one of the main reasons for the increase of the degree of brain injury.The established LSCI method can be applied to real-time monitoring of MCAO rat cerebral blood flow, which is easy to operate, sensitive, stable and reliable.
Cerebral ischemia-reperfusion / Laser speckle imaging / Cerebral blood flow
[1] Mitchell P, Wang JJ, Wong TY, et al. Retinal microvascular sign sand risk of stroke and stroke mortality[J]. Neurology, 2005, 65(7):1005-1009.
[2] 闵喆, 李昌盛, 方瑜, 等. 激光散斑成像技术在大鼠大脑中动脉栓塞模型侧枝循环观测中的应用[J]. 神经损伤与功能重建, 2014, 9(3): 191-194.
[3] Hossmann KA. Reperfusion of the brain after global ischemia: hemodynamic disturbances[J]. Shock, 1997,8(2):95-101.
[4] Hoyte LC, Papadakis M, Barber PA, et al. Improved regional cerebral blood flow is important for the protection seen in a mouse model of late phase ischemic preconditioning[J]. Brain Res, 2006,1121(1):231-237.
[5] Takaharu F, Yasuo K, Tatsushi K, et al. The effect of duration of cerebral ischemia on brain pyruvate dehydrogenase activity, energy metabolites, and blood flow during reperfusion in gerbil brain [J]. Brain Research, 1998, 792(1):59-65.
[6] Kuroiwa T, Bonnekon P, Hossmann KA. Laser Doppler flowmetry in CA1 sector of hippocampus and cortex after transient forebrain ischemia in gerbils [J]. Stroke, 1992, 23（9）：1349-1354.
[7] Dziennis S, Qin J, Shi L, et al. Macro-to-micro cortical vascular imaging underlies regional differences in ischemic brain[J]. Sci Rep, 2015, 5:1-13.
[8] Sheu WH, Chuang HC, Cheng SM, et al. Microdialysis combined blood sampling technique for the determination of rosiglitazone and glucose in brain and blood of gerbils subjected to cerebral ischemia[J]. J Pharm Biomed Anal, 2011, 54(4):759-764.
[9] 朱淑萍. 大鼠大脑中动脉栓塞模型早期脑血流变化的研究[D]. 上海交通大学, 2012.
[10] Erik FH, Sebastian A, Julie FH, et al. Capillary flow and diameter changes during reperfusion after global cerebral ischemia studied by intravital video microscopy [J]. Cereb Blood Flow Metab, 2004, 24(4):383-391.
[11]廖卫国. UPLC-MS/LSCI法补阳还五汤大鼠药动学研究及药效探索[D]. 广州中医药大学, 2016.
[12]曹英环, 余健烨, 廖卫国. 激光散斑成像技术测定阿魏酸对大鼠脑血流量的影响[J].北方药学, 2016,13(2):136-137.
[13]刘谦. 激光散斑衬比成像技术及其应用的研究[D].华中科技大学, 2005:78-79.
国家自然科学基金(81373973, 81573872); 广东省科技计划项目(2013B032500015)
京ICP备08002354号-3/
| 〈 |
|
〉 |
