樟芝酵素对苯丙胺致PC12细胞毒性损伤的保护作用
The protective effect and its mechanism of Antrodia camphorata enzyme on the cytotoxicity injury of PC12 cells induced by amphetamine
目的 探讨樟芝酵素对苯丙胺致PC12细胞损伤的保护作用及其机理。 方法 利用PC12细胞模型，设置对照组，3.5 mmol/L苯丙胺组和3.5 mmol/L苯丙胺+樟芝酵素组。(1)通过显微镜观察细胞形态的变化；(2)通过MTT法检测细胞存活率；(3)通过Western blot分析Caspase-3的变化; (4)通过流式细胞仪检测细胞凋亡和细胞内活性氧的变化。 结果 (1)苯丙胺损伤了PC12细胞的结构，导致细胞凋亡增加，樟芝酵素能够减少细胞的凋亡，提高细胞的存活率；(2)樟芝酵素减少了细胞内ROS的生成；(3)樟芝酵素组procaspase-3（35 kd）蛋白含量比苯丙胺组增多，激活分子caspase-3（17 kd）含量较苯丙胺组减少。 结论 樟芝酵素对苯丙胺致PC12细胞毒性损伤具有保护作用，其机理与抑制细胞线粒体凋亡信号通路以及减少细胞内氧自由基的产生密切相关。
Objective To investigate the protective effect and its mechanism of Antrodia camphorata enzyme on the cytotoxicity injury of PC12 cells induced by Amphetamine (AMPH). Methods PC12 cells were treated with 3.5 mmol/L AMPH or 3.5 mmol/L AMPH pus Antrodia camphorata enzyme. The morphological changes of the cells were observed under the light microscope. Cell viability assay was measured by MTT method. Caspase-3 levels were detected by Western blot, and the changes of reactive oxygen species（ROS） and apoptosis of cells were analyzed by flow cytometer. Results (1)AMPH damaged structure of PC12 cells, and resulted in cell apoptosis. Antrodia camphorata enzyme reduced cell apoptosis, and increased the survival rate of cells. (2)Antrodia camphorata enzyme reduced generation of ROS in PC12 cells. (3)Compared to AMPH group, procaspase-3 (35 kd) level was increased while procaspase-3 (17 kd) level was reduced in Antrodia camphorata enzyme group. Conclusion Antrodia camphorata enzyme has a protective effect on the cytotoxicity of PC12 cells induced by AMPH. Its mechanism may be related to inhibition of the mitochondrial apoptosis signal pathway and reduction of reactive oxygen species in cells.
樟芝酵素 / 苯丙胺 / PC12细胞 / 细胞凋亡 / 活性氧
Antrodia camphorata enzymes / Amphetamine; / PC12 cell; / Apoptosis; / Reactive oxygen species
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国家自然科学基金项目（81470055）; 暨南大学大学生创新创业训练计划项目(CX15181)
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