施万样细胞对大鼠脊神经节NGF 和BDNF 表达的影响

付秀美; 王荣良; 杨振江; 付文亮; 王小杰

doi:10.13418/j.issn.1001-165x.2017.01.010

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (1) : 48-51. DOI: 10.13418/j.issn.1001-165x.2017.01.010

实验研究

施万样细胞对大鼠脊神经节NGF 和BDNF 表达的影响

付秀美¹，　王荣良²，　杨振江¹，　付文亮¹，　王小杰³

作者信息 +

Effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury

FU Xiu-mei¹, WANG Rong-liang², YANG Zhen-jiang¹, FU Wen-liang¹, WANG Xiao-jie³

Author information +

文章历史 +

摘要

目的　观察施万样细胞对坐骨神经损伤（sciatic nerve injury，SNI）大鼠脊神经节NGF 和BDNF 表达的影响，初步探讨施万样细胞对脊神经节的保护作用。方法　先将脂肪源性干细胞（adipose-derived stem cells，ADSCs）诱导分化为施万样细胞并对后者进行鉴定，后将二者分别植入脱细胞神经移植物（ANA）中，构建组织工程神经。大鼠随机分为正常对照组、ADSC 组和施万样细胞组。后两组建立SNI 模型并用相应的组织工程神经桥接损伤的神经。术后4 周采用Western Blot 和Real-time PCR 检测各组大鼠脊神经节神经生长因子（nerve growth factor，NGF）和脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）蛋白和mRNA 的表达。结果　ADSCs 能够诱导分化为施万样细胞并表达施万细胞标记物S100β 和GFAP 蛋白。施万样细胞组大鼠脊神经节内NGF 和BDNF 蛋白及mRNA 表达量均高于ADSC 组（P＜0.05）。结论　施万样细胞可上调脊神经节NGF 和BDNF 的表达，对SNI 所致的脊神经节内神经元损伤有保护作用。

Abstract

Objective　To investigate the effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury (SNI) and explore the protective effects of Schwann-like cells on spinal ganglion.　Methods　Firstly，the adipose derived stem cells (ADSCs) were differentiated into Schwann-like cells and the phenotype of the differentiated cells were identified, then the ADSCs and Schwann-like cells were implanted into the acellular nerve grafts (ANA), so the tissue engineering nerves were constructed. The rats were randomly divided into 3 groups: normal control group, ADSC group and Schwann-like cells group. The SNI model was established in rats of the latter two groups, and then the corresponding tissue engineering nerves were used to bridge the injured sciatic nerves. 4 Weeks after operation, Western Blot and Real-time PCR were used to detect the expression of NGF and BDNF protein and mRNA in spinal ganglia.　Results　ADSCs can be induced to differentiate into Schwann-like cells and expression of S100β and GFAP protein (markers of Schwann cells). In contrast with ADSC group, the expressions of NGF and BDNF protein and mRNA in Schwann-like cells group rats increased (P＜0.05). Conclusion　 Schwann-like cells can enhance the expression of NGF and BDNF in the spinal ganglia, so they can have protective effects on the spinal ganglia in rats with SNI.

导出引用

付秀美,王荣良,杨振江,付文亮,王小杰. 施万样细胞对大鼠脊神经节NGF 和BDNF 表达的影响[J]. 中国临床解剖学杂志. 2017, 35(1): 48-51 https://doi.org/10.13418/j.issn.1001-165x.2017.01.010

Effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury[J]. Chinese Journal of Clinical Anatomy. 2017, 35(1): 48-51 https://doi.org/10.13418/j.issn.1001-165x.2017.01.010

参考文献

[1] Liu G, Cheng Y, Guo S, et al. Transplantation of adipose-derived stem cells for peripheral nerve repair[J]. Int J Mol Med, 2011, 28(4):565-572.
[2] Zheng M, Duan J, He Z, et al. Overexpression of tropomyosin receptor kinase A improves the survival and Schwann-like cell differentiation of bone marrow stromal cells in nerve grafts for bridging rat sciatic nerve defects[J]. Cytotherapy, 2016,18(10):1256-1269.
[3] Fu X, Tong Z, Li Q, et al. Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation[J]. Mol Med Rep, 2016,14(2):1187-1193.
[4] 崔慧先. 系统解剖学[M]. 北京：人民卫生出版社，2009：304-319.
[5] Wu Y, Wang L, Guo B, et al. Electroactive biodegradable polyurethane significantly enhanced Schwann cells myelin gene expression and neurotrophin secretion for peripheral nerve tissue engineering[J]. Biomaterials, 2016,87:18-31.
[6] Li HF, Wang YR, Huo HP, et al. Neuroprotective effects of ultrasound-guided nerve growth factor injections after sciatic nerve injury[J]. Neural Regen Res, 2015, 10(11):1846-1855.
[7] Li S, Wang X, Gu Y, et al. Let-7 microRNAs regenerate peripheral nerve regeneration by targeting nerve growth factor[J]. Mol Ther, 2015, 23(3):423-433.
[8] Xu C, Bai L, Chen Y, et al. Effect of mutated defensin NP-1 on sciatic nerve regeneration after transection-A pivot study[J]. Neurosci Lett, 2016,617:283-287.
[9] Hannan JL, Albersen M, Stopak BL, et al. Temporal changes in neurotrophic factors and neurite outgrowth in the major pelvic ganglion following cavernous nerve injury[J]. J Neurosci Res, 2015, 93(6):954-963.
[10]Lin G, Zhang H, Sun F, et al. Brain-derived neurotrophic factor promotes nerve regeneration by activating the JAK/STAT pathway in Schwann cells[J]. Transl Androl Urol, 2016, 5(2):167-175.
[11]Zheng J, Sun J, Lu X , et al. BDNF promotes the axonal regrowth after sciatic nerve crush through intrinsic neuronal capability upregulation and distal portion protection[J]. Neurosci Lett, 2016, 621:1-8.