人脂肪源干细胞促进前交叉韧带重建后腱骨界面愈合的实验研究
陈平,曲戎梅,韩忠宇,杨宇超,尹玉琨,田京,张力
中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (6) : 643-646.
人脂肪源干细胞促进前交叉韧带重建后腱骨界面愈合的实验研究
The study of human adipose-derived stem cells in promoting the tendon-bone healing in a rabbit anterior cruciate ligament reconstruction model
目的 探讨人脂肪源干细胞（human adipose-derived stem cells, hASCs）能否促进前交叉韧带重建后腱骨界面的愈合。 方法 将30只体重2.0~2.5 kg的雌性新西兰大白兔随机分为实验组和对照组，每组各15只。取同侧半腱肌作为供体肌腱，建立单侧下肢前交叉韧带重建动物模型，实验组和对照分别在胫骨和股骨隧道中注射hASCs或等量的生理盐水。分别在术后2、4和8周取材，收集胫骨-移植韧带-股骨标本行大体观察和组织学观察。 结果 HE染色示对照组术后2周腱骨界面主要以炎性细胞浸润为主，4周后炎性细胞减少，8周时腱骨界面可见Sharpey纤维，形成间接止点。实验组术后4周腱骨界面可见纤维软骨细胞及少许骨细胞形成，术后8周可见新骨形成,形成直接止点的结构。 结论 hASCs能促进前交叉韧带重建后腱骨界面的骨化作用，形成直接止点结构，从而促进腱骨界面的愈合。
Objective To investigate whether human adipose-derived stem cells (hACSs) can promote tendon-bone healing. Methods Unilateral anterior cruciate ligament reconstruction using autologous semitendinosus tendons were conducted on 30 New Zealand rabbits(weighing 2~2.5kg), which were randomly divided into 2 groups. Rabbits in the experimental group were injected with hASCs in the tibial and femoral bone tunnels while rabbits in the control group were injected with equal normal saline. All experimental animals were sacrificed at 2 weeks, 4 weeks and 8 weeks after surgery. The specimens were harvested for gross observation and histological analysis. Results At 2 weeks after operation in the control group, a lot of inflammatory cells infiltrated the tendon-bone interface while the inflammatory cells decreased at 4 weeks. At 8 weeks postoperatively, sharpey’s fibers were found in the interface forming indirect insertion. In the experimental group, the tendon-bone interface was filled with fibro-cartilage cells and a few osteoblasts at 4 weeks after surgery. New bones can be found in the tendon-bone interface at 8 weeks postoperatively in the experimental group and formed the direct insertion. Conclusion hASCs can promote tendon-bone healing by forming a direct insertion in a rabbit anterior cruciate ligament reconstruction model.
Human adipose-derived stem cells / Anterior cruciate ligament reconstruction / Tendon-bone healing
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