丝线法制备豚鼠不完全性小肠梗阻模型及对c-kit表达的影响研究

杨拯; 李欣芮; 王强; 刘双; 黄倩; 计震华

doi:10.13418/j.issn.1001-165x.2016.04.014

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (4) : 423-427. DOI: 10.13418/j.issn.1001-165x.2016.04.014

实验研究

丝线法制备豚鼠不完全性小肠梗阻模型及对c-kit表达的影响研究

杨拯，　李欣芮，　王强，　刘双，　黄倩，　计震华

作者信息 +

Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method

YANG Zheng, LI Xin-rui, WANG Qiang, LIU Shuang, HUANG Qian, JI Zhen-hua

Author information +

文章历史 +

摘要

目的　探讨丝线法建立豚鼠不完全性小肠梗阻模型对ICC细胞表达c-kit的影响。方法　选取43只成年健康豚鼠随机分成正常A组、假手术B组、模型组（C、D、E）。对模型组豚鼠通过丝线法造成不完全性肠梗阻,3 d后取出丝线并关闭腹腔,分别于术后第1d（C组）,第3d（D组）,第7d（E组）对小肠收缩力、肠黏膜损伤程度及小肠c-kit含量变化进行测定。结果　模型组各项指标的测定与正常组比较均有统计学差异（P<0.05）,但E组肠黏膜损伤程度与A组比较无明显差异（P>0.05）。E组小肠收缩力较D组高（P<0.05），肠黏膜损伤程度较D组轻（P<0.01），小肠c-kit含量较D组明显增高（P<0.01）。结论　丝线法能建立豚鼠不完全性肠梗阻模型，同时可降低c-kit蛋白表达。

Abstract

Objective　To investigatethe expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models in guinea pig with the silk method.　Methods　43 adult healthy guinea pigs were selected and randomly divided into the normal group A, sham-operated group B, model groups（C, D, E）. Model groups were established for incomplete small intestinal obstruction by the means of silk method for 3 days. After taking out of the silk and closing the abdominal cavity, the abdominal cavity was opened and the intestinal contractility was measured，respectively at 1st（C group）, 3rd（D group）,7th（E group）. Meanwhile, the contractility of intestinal tissue was measured, the degree of intestinal mucosal membrane injury was evaluated and the expression of c-kit was observed.　Results　The comparison of normal and model groups in every data’s measurement were significant on statistics (P<0.05),but the comparison of intestinal mucosal membrane injury between E and A group had no significant difference (P>0.05). The contractility of intestinal tissue of E group was higher than D group (P<0.05), and the degree of intestinal mucosal membrane injury was less serious than D group (P<0.01). Besides, the expression of c-kit in E group wass significantly higher than D group (P<0.01). Conclusion　Incomplete intestinal obstruction with down-regulated c-kit could be established by the silk method.

导出引用

杨拯，李欣芮，王强，刘双，黄倩，计震华. 丝线法制备豚鼠不完全性小肠梗阻模型及对c-kit表达的影响研究[J]. 中国临床解剖学杂志. 2016, 34(4): 423-427 https://doi.org/10.13418/j.issn.1001-165x.2016.04.014

YANG Zheng, LI Xin-rui, WANG Qiang, LIU Shuang, HUANG Qian, JI Zhen-hua. Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method[J]. Chinese Journal of Clinical Anatomy. 2016, 34(4): 423-427 https://doi.org/10.13418/j.issn.1001-165x.2016.04.014

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