维生素C对大鼠背根神经节神经元突起生长的影响

李媛媛; 文锦坤; 潘梦婕; 李莉霞; 王祥海; 钱长晖; 郭家松

doi:10.13418/j.issn.1001-165x.2016.03.015

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (3) : 308-311. DOI: 10.13418/j.issn.1001-165x.2016.03.015

实验研究

维生素C对大鼠背根神经节神经元突起生长的影响

李媛媛¹，　文锦坤¹，　潘梦婕¹，　李莉霞¹，　王祥海¹，　钱长晖¹，　郭家松^1,2

作者信息 +

The effect of vitamin C on the neurite outgrowth of dorsal root ganglionic neurons

LI Yuan-yuan¹, WEN Jin-kun¹, PAN Meng-jie¹, LI Li-xia ¹, WANG Xiang-hai¹, QIAN Chang-hui¹, GUO Jia-song ^1,2

Author information +

文章历史 +

摘要

目的　研究维生素C（Vitamin C，Vit.C）对大鼠背根神经节神经元轴突生长的影响，为探讨神经再生新策略提供基础。方法　取新生SD大鼠背根神经节（dorsal root ganglion, DRG）进行神经元分离培养。在培养液中加入不同浓度的维生素C（0, 100, 200, 400 μmol/L）处理24 h后通过βIII tubulin, RhoA免疫组化以及鬼笔环肽染色对神经元突起的数量和长度、生长锥伪足数量、神经元胞体大小及其内RhoA表达强度进行分析。结果神经元突起的数量、长度，生长锥伪足的数量以及神经元胞体面积均随维生素C的浓度增高而增长，而神经元内RhoA表达强度则相应降低。结论维生素C可促进体外大鼠背根节神经元突起的生长，这可能与RhoA表达下调相关。

Abstract

Objective　To study the effect of Vitamin C on the neurite outgrowth of dorsal root ganglionic neurons.　Methods　Neurons harvested from the dorsal root ganglion of newborn SD rat pups were cultured in a basic medium supplemented with a series of concentration of Vitamin C (0, 100, 200, 400μmol/L). 24 hours later, the cultures were immunostained with antibodies of βIII tubulin and RhoA. The βIII tubulin immunostained cultures were double stained with phalloidin. Then, the number and length of the neurites, number of pseudopodium in the growth cone of the cultured neurons, as well as the immunofluorence intensity of RhoA in the neuronal bodies were quantified.　Results　The number and length of the neurites, number of pseudopodium in the growth cone of the cultured neurons were all increased in Vit. C treated groups compared with the control group. Howerver， the RhoA level was decreased in the Vit. C treated groups.　Conclusion　Vit.C could promote the neurite outgrowth of cultured dorsal root ganglionic neurons which may result in reducing the expression of RhoA.

导出引用

李媛媛,文锦坤,潘梦婕,李莉霞,王祥海,钱长晖,郭家松. 维生素C对大鼠背根神经节神经元突起生长的影响[J]. 中国临床解剖学杂志. 2016, 34(3): 308-311 https://doi.org/10.13418/j.issn.1001-165x.2016.03.015

LI Yuan-yuan, WEN Jin-kun, PAN Meng-jie, LI Li-xia,WANG Xiang-hai, QIAN Chang-hui, GUO Jia-song. The effect of vitamin C on the neurite outgrowth of dorsal root ganglionic neurons[J]. Chinese Journal of Clinical Anatomy. 2016, 34(3): 308-311 https://doi.org/10.13418/j.issn.1001-165x.2016.03.015

参考文献

[1] Alagl AS, Bhat SG. Ascorbic acid: new role of an age-old micronutrient in the management of periodontal disease in older adults[J]. Geriatr Gerontol Int, 2015,15(3):241-254.
[2] Pardo B, Mena MA, Casarejos MJ, et al. Toxic effects of L-DOPA on mesencephalic cell cultures: protection with antioxidants[J]. Brain Res, 1995, 682 (1-2):133-143.
[3] Yan M, Yang M, Shao W, et al. High-dose ascorbic acid administration improves functional recovery in rats with spinal cord contusion injury[J]. Spinal Cord, 2014,52 (11):803-808.
[4] Lu R, Kallenborn-Gerhardt W, Geisslinger G, et al. Additive antinociceptive effects of a combination of vitamin C and vitamin E after peripheral nerve injury[J]. PLoS One, 2011,6 (12):e29240.
[5] Harrison FE, May JM. Vitamin C function in the brain: vital role of the ascorbate transporter SVCT2[J]. Free Radic Biol Med, 2009, 46 (6):719-730.
[6] Sango K, Kawakami E, Yanagisawa H, et al. Myelination in coculture of established neuronal and Schwann cell lines[J]. Histochem Cell Biol, 2012,137 (6):829-839.
[7] 韩泽民, 郭家松. 神经元内抑制神经再生的相关信号通路[J]. 生命科学, 2015, 27(9): 1120-1124.
[8] Chen J, Guo L, Zhang L, et al. Vitamin C modulates TET1 function during somatic cell reprogramming[J]. Nat Genet, 2013, 45 (12):1504-1509.
[9] Passage E, Norreel JC, Noack-Fraissignes P, et al. Ascorbic acid treatment corrects the phenotype of a mouse model of Charcot-Marie-Tooth disease[J]. Nat Med, 2004,10 (4):396-401.
[10] Engelhart MJ, Geerlings MI, Ruitenberg A, et al. Dietary intake of antioxidants and risk of Alzheimer disease[J]. Jama, 2002, 287(24):3223-3229.
[11] Fujita Y, Yamashita T. Axon growth inhibition by RhoA/ROCK in the central nervous system[J]. Front Neurosci, 2014, 8:338-349.
[12] Palazzolo G, Horvath P, Zenobi-Wong M. The flavonoid isoquercitrin promotes neurite elongation by reducing RhoA activity[J]. PloS one, 2012, 7(11):e49979.