茶多酚对慢性酒精中毒肝损伤影响的实验研究

汤银娟; 王建钧; 王祥海; 潘梦婕; 陈卓; 肖玲珑; 刘嘉华; 蔡维君

doi:10.13418/j.issn.1001-165x.2016.02.016

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (2) : 198-201. DOI: 10.13418/j.issn.1001-165x.2016.02.016

实验研究

茶多酚对慢性酒精中毒肝损伤影响的实验研究

汤银娟^1,2，　王建钧³，　王祥海⁴，　潘梦婕⁴，　陈卓³，　肖玲珑³，　刘嘉华³，　蔡维君¹

作者信息 +

The research of tea polyphenols for liver failure due to chronic alcoholic liver damage

TANG Yin-juan ^1,2，WANG Jian-jun ³，WANG Xiang-hai ⁴， PAN Meng-jie ⁴，CHEN Zhuo ³, XIAO Ling-long³, LIU Jia-hua ³, CAI Wei-jun ¹

Author information +

文章历史 +

摘要

目的　探讨茶多酚在预防慢性酒精中毒对肝损伤方面的作用及其可能的机制。方法　将40只雄性昆明小鼠随机分成正常对照组、酒精组、茶多酚低剂量组和茶多酚高剂量组。酒精组、茶多酚组连续90 d喂服30%酒精，8 h后再分别喂服纯水，浓度1%、2%的茶多酚悬液。正常对照组以同样方式和剂量每天2次喂服纯水。末次灌胃24 h检测血清中谷草转氨酶（AST）、谷丙转氨酶（ALT）的含量和肝组织中超氧化物歧化酶SOD、丙二醛（MDA）、组织内活性氧类物质（ROS）、谷胱甘肽过氧化物酶（GSH-px）的水平；同时，取肝组织行免疫组织化学显示Bcl-2和Bax的表达。结果　与正常对照组比较，酒精损伤组肝细胞Bax阳性表达均明显升高（P＜0.01），肝细胞Bcl-2阳性表达明显降低（P＜0.01）。茶多酚喂服的两组有所改善。结论　慢性酒精中毒可以引起肝损伤，而喂服茶多酚能减轻损伤程度。

Abstract

Objective　To investigate the preventive effect of tea polyphenols from liver injury in chronic alcoholism, and explore its possible molecular mechanisms.　Methods　40 male kunming mice were divided into a control group, an alcohol group, a low dose group and a high dose group randomly. The mice model of alcoholic liver injury was duplicated by gavage and was given Alcohol and tea polyphenol respectively, using pure water as the control group. Then the mice′s AST, ALT, SOD, MDA, ROS, GSH-px,Bcl-2 and Bax of blood serum and liver tissue in each group weredetermined.　Result　The treatment medicine indicated that both the high or low doses of tea polyphenols could reduce the model of mice′s liver damage. Conclusion　The tea polyphenols could alleviate liver injury through inhibiting the preoxidation and eliminating free radicals.

导出引用

汤银娟,王建钧,王祥海,潘梦婕,陈卓,肖玲珑,刘嘉华,蔡维君. 茶多酚对慢性酒精中毒肝损伤影响的实验研究[J]. 中国临床解剖学杂志. 2016, 34(2): 198-201 https://doi.org/10.13418/j.issn.1001-165x.2016.02.016

TANG Yin-juan，WANG Jian-jun,WANG Xiang-hai,PAN Meng-jie,CHEN Zhuo,XIAO Ling-long, LIU Jia-hua, CAI Wei-jun. The research of tea polyphenols for liver failure due to chronic alcoholic liver damage[J]. Chinese Journal of Clinical Anatomy. 2016, 34(2): 198-201 https://doi.org/10.13418/j.issn.1001-165x.2016.02.016

参考文献

[1] 齐慧慧，宋佳，陈岳祥. 小鼠急性酒精性肝损伤模型的建立[J]. 世界华人消化杂志, 2012, (9): 759-763.
[2] 任洁. 硫酸锌/枸杞多糖对大鼠慢性酒精性肝损伤的协同保护作用[D]. 西北农林科技大学, 2014.
[3] 何双艳，李昌平，李鹏，等. Fas/FasL、Bcl-2/Bax、Caspase-8在大鼠非酒精性脂肪性肝病中的作用机制[J]. 世界华人消化杂志, 2012, (36): 3761-3766.
[4] 石海燕. BCL-2，NF-kB，血管紧张素I I-I受体在大鼠非酒精性脂肪肝病中的作用及其机制研究[D]. 泸州医学院, 2013.
[5] 庄甲举，王憬，王志玲，等. Bcl-2、Bax基因表达在大鼠免疫性肝损伤中作用的探讨[J]. 卫生研究, 2005, 34 (6): 716-719.
[6] Arend N， Wertheimer C， Laubichler P，et al. Idebenone prevents oxidative stress, cell death and senescence of retinal pigment epithelium cells by stabilizing BAX/Bcl-2 ratio[J]. Ophthalmologica, 2015, 234(2):78-82.
[7] Albuquerque KF， Marinovic MP， Morandi AC，et al. Green tea polyphenol extract in vivo attenuates inflammatory features of neutrophils from obese rats[J]. Eur J Nutr, 2015. [Epub ahead of print]
[8] 罗艳蕊，王建设，崔迪. 茶多酚对营养性肥胖大鼠肝脏自由基代谢的影响[J]. 中国应用生理学杂志, 2012, (3): 196-198.
[9] 汤银娟，王建钧，王祥海，等. 茶多酚预防慢性酒精中毒对精子损伤的作用及机制研究[J]. 中国临床解剖学杂志, 2014, 32(5): 604-608.
[10] 张旭. 益肝颗粒防治大鼠酒精性肝损伤的作用机制研究[D]. 辽宁中医药大学, 2013.
[11] Ji C. Advances and new concepts in alcohol-induced organelle stress, unfolded protein responses and organ damage[J]. Biomolecules, 2015, 5(2): 1099-1121.
[12] Cook PA， Morleo M， Billington D，et al. Evaluation of work-based screening for early signs of alcohol-related liver disease in hazardous and harmful drinkers: the PrevAIL study[J]. BMC Public Health, 2015, 15: 532.
[13] 王媛，荣华，初晓辉，等. 普洱茶提取物及普洱茶多糖对人成纤维细胞抗衰老作用机制研究[J]. 云南农业大学学报(自然科学), 2015, 30(2): 219-227.
[14] 刘丽. 黄芪甲苷对D-GalN/LPS诱导小鼠急性肝衰竭模型Nrf2/HO-1和Bcl-2/Bax表达的影响[D]. 南华大学, 2014.
[15] Karmakar I， Haldar S， Chakraborty M，et al. Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage by Zanthoxylum alatum[J]. Pharm Biol, 2016, 54(3):503-508.
[16] 仲文秀. 大鼠慢性酒精性肝损伤时NF-κB、TNF-α、COX-2的表达[D]. 延边大学, 2012.
[17] Aboutaleb N， Shamsaei N， Khaksari M，et al. Pre-ischemic exercise reduces apoptosis in hippocampal CA3 cells after cerebral ischemia by modulation of the Bax/Bcl-2 proteins ratio and prevention of caspase-3 activation[J]. J Physiol Sci, 2015, 65(5):435-443.

基金

湖南省教育厅课题（15C1276）；湘南学院大学生研究性学习与创新性实验项目（湘南学院校发【2013】155号）；湘南学院“橘井泉香”科技文化节项目（2014KJJ06）；国家级大学生研究性学习和创新性实验项目（教高司函[2015]41号）；湖南省大学生研究性学习和创新性实验项目（湘教通[2015]269号）。