Wistar大鼠口腔颌面部微血管铸型制作
Researching microvascular casting model of oral and maxillofacial regions of Wistar rats
目的 通过Wistar大鼠头面部微血管铸型标本制作与扫描电镜观察，旨在建立毛细血管真实的三维空间显示方法，以便于毛细血管三维数字化重建。 方法 8周龄Wistar大鼠从腹腔以下断除，从主动脉插管冲洗后，灌注树脂材料。恒温水浴箱放置24 h后，解剖大鼠头颈部，形成皮肤标本、舌标本、下颌骨标本、上颌骨标本。将标本腐蚀、冲洗、干燥。用离子镀膜机进行喷镀金，在扫描电镜下观察血管铸型的解剖形态、走形、以及毗邻结构。 结果 成功制备了Wistar大鼠的皮肤标本、舌标本、下颌骨标本、上颌骨标本的毛细血管铸型，清晰显示局部毛细血管的形态与走形结构，扫描电镜下可显示微观毛细血管三维空间结构。 结论 建立了Wistar大鼠头面部的毛细血管铸型方法，为研究微血管的形态学三维重建提供了方法，也为毛细血管的3D打印奠定了基础。
Objectives To build the capillaries casting method of Wistar rats maxillofacial microvascular specimens by perfusion combined with scanning electron microscopy (SEM), so that the three-dimensional digital models of capillaries were established. Methods Maxillofacial microvascular specimens of 8 weeks of Wistar rats were resected from the abdominal cavity. The catheter was inserted from ventricle to the aorta. The resin material was filled, and specimen was placed in constant temperature water bath for 24 hours. The sample was divided into some parts, such as skin, tongue, jaw bones. The samples were corroded, washed, dried, and plated gold. The anatomical morphology of vascular casting was observed under scanning electron microscopy, course and adjacent structures. Results Capillary casting specimens of skin, tongue, mandible and maxilla of Wistar rats were successfully prepared. They clearly showed the morphology and course of the capillaries in the specimens. Under the scanning electron microscopy, the micro capillary structures of the capillaries were showed in three dimensions. Conclusion The facial capillaries casting methods in Wistar rats can serve as the basis for 3D digital model of the microvascular morphology and 3D printing of the capillaries.
Microcirculation / Wistar rat / Microvascular casting / Model
[1] Titterington JS, Hung OY, Wenger NK. Microvascular angina: an update on diagnosis and treatment [J]. Future Cardiol, 2015, 11(2):229-242.
[2] Glancy B, Hsu LY, Dao L, et al. In vivo microscopy reveals extensive embedding of capillaries within the sarcolemma of skeletal muscle fibers [J]. Microcirculation, 2014, 21(2):131-147.
[3] Astapenko D, Jor O, Lehmann C, et al. In situ assessment of the renal microcirculation in mechanically ventilated rats using sidestream dark-field imaging [J]. J Microsc, 2015, 257(2):161-165.
[4]王爱平, 彭田红, 周小兵, 等. 颌面部血管的应用解剖[J]. 中国临床解剖学杂志, 2010, 28(6):606-610.
[5] 李少华, 张业辉, 陈胜华, 等. 以示指桡掌侧筋膜皮支为蒂逆行岛状皮瓣的应用解剖[J]. 中国临床解剖学杂志, 2008, 26(3):278-279.
[6] Jiao HS, Shan T, Cheng GL, et al. A novel blood supply method for free jejunal transfer with the third jejunal artery as vascular pedicle [J]. Surg Radiol Anat, 2012, 34(10):953-957.
[7] Chang CP. Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting [C]. Methods Mol Biol, 2012, 843:21-28.
[8] Kajiwara N, Masaki C, Mukaibo T, et al. Soft tissue biological response to zirconia and metal implant abutments compared with natural tooth: microcirculation monitoring as a novel bioindicator [J]. Implant Dent, 2015, 24(1):37-41.
[9] 廖进民, 王兴海, 李忠华, 等.下颌骨血供及血管构筑的临床解剖学研究[J]. 中国临床解剖学杂志, 2005, 23(1):27-30.
[10]Selliseth NJ, Selvig KA. Microvascular adaptation to transmucosal implants. A scanning electron microscopic study in the rats [J]. Clin Oral Implants Res, 1995, 6(4):205-212.
[11]Kishi Y, Takahashi K, Trowbridge H. Vascular network in papillae of dog oral mucosa using corrosive resin casts with scanning electron microscopy [J]. Anat Rec, 1990, 226 (4):447-459.
[12]Dickie R, Bachoo RM, Rupnick MA, et al. Three-dimensional visualization of microvessel architecture of whole-mount tissue by confocal microscopy [J]. Microvasc Res, 2006, 72(1-2):20-26.
国家自然科学基金（31170903）
京ICP备08002354号-3/
| 〈 |
|
〉 |
